Activity and Mechanism of Citri grandis Exocarpium-Cordyceps militaris Compound on Reliving Cough Based on Network Pharmacology
This study aimed to investigate the activity and mechanism of Citri grandis Exocarpium-Cordyceps militaris compound on reliving cough by the use of network pharmacology. Mouse cough inducing models were constructed by ammonia water to evaluate the cough suppressant activity of the compound. Multiple...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | zho |
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The editorial department of Science and Technology of Food Industry
2022-07-01
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| Series: | Shipin gongye ke-ji |
| Subjects: | |
| Online Access: | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100261 |
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| author | Chunwei JIAO Jing ZHONG Haiyong HUANG Huijia LIANG Jiaoji ZHENG Jiaming CHEN Yizhen XIE |
| author_facet | Chunwei JIAO Jing ZHONG Haiyong HUANG Huijia LIANG Jiaoji ZHENG Jiaming CHEN Yizhen XIE |
| author_sort | Chunwei JIAO |
| collection | DOAJ |
| description | This study aimed to investigate the activity and mechanism of Citri grandis Exocarpium-Cordyceps militaris compound on reliving cough by the use of network pharmacology. Mouse cough inducing models were constructed by ammonia water to evaluate the cough suppressant activity of the compound. Multiple online databases and literature searches were then used to collect the active ingredients, targets of action and disease targets of the compound. Construction of compound-target-disease networks and protein interaction networks using Cytoscape and STRING, and GO and KEGG enrichment analysis of target genes using Meatscape. The results showed that the low and high dose groups significantly reduced the number of coughs in mice (P<0.001) and decreased the levels of three inflammatory factors, tumor necrosis factor, interleukin 1α and interleukin 1β in mice serum, with significant differences in the high dose group (P<0.05, P<0.01, P<0.05). The results of the network pharmacological analysis showed that this compound could act on 78 targets including transcription factor AP-1, interleukin 6 and tumor necrosis factor, regulating 575 GO entries and 272 signalling pathways to exert cough suppressant effects, of which cordycepic acid, cordycepin, apigenin, beta-sitosterol and naringenin were the important material basis. The results indicated that the combination of Citri grandis Exocarpium-Cordyceps militaris has cough suppressant activity, which was the result of the combined action of multiple substances in the combination on multiple targets and pathways. |
| first_indexed | 2024-04-12T08:48:17Z |
| format | Article |
| id | doaj.art-4bb189b9d11542fcb3ea40cf38aff63e |
| institution | Directory Open Access Journal |
| issn | 1002-0306 |
| language | zho |
| last_indexed | 2024-04-12T08:48:17Z |
| publishDate | 2022-07-01 |
| publisher | The editorial department of Science and Technology of Food Industry |
| record_format | Article |
| series | Shipin gongye ke-ji |
| spelling | doaj.art-4bb189b9d11542fcb3ea40cf38aff63e2022-12-22T03:39:38ZzhoThe editorial department of Science and Technology of Food IndustryShipin gongye ke-ji1002-03062022-07-014313172410.13386/j.issn1002-0306.20211002612021100261-13Activity and Mechanism of Citri grandis Exocarpium-Cordyceps militaris Compound on Reliving Cough Based on Network PharmacologyChunwei JIAO0Jing ZHONG1Haiyong HUANG2Huijia LIANG3Jiaoji ZHENG4Jiaming CHEN5Yizhen XIE6Guangdong Yuewei Biotechnology Co., Ltd., Zhaoqing 512000, ChinaGuangdong Yuewei Edible Fungi Technology Co., Ltd., Guangzhou 510525, ChinaGuangdong Nan Yue Pharmaceutical Co., Ltd., Guangzhou 510530, ChinaGuangdong Yuewei Edible Fungi Technology Co., Ltd., Guangzhou 510525, ChinaGuangdong Yuewei Edible Fungi Technology Co., Ltd., Guangzhou 510525, ChinaGuangdong Yuewei Edible Fungi Technology Co., Ltd., Guangzhou 510525, ChinaGuangdong Yuewei Edible Fungi Technology Co., Ltd., Guangzhou 510525, ChinaThis study aimed to investigate the activity and mechanism of Citri grandis Exocarpium-Cordyceps militaris compound on reliving cough by the use of network pharmacology. Mouse cough inducing models were constructed by ammonia water to evaluate the cough suppressant activity of the compound. Multiple online databases and literature searches were then used to collect the active ingredients, targets of action and disease targets of the compound. Construction of compound-target-disease networks and protein interaction networks using Cytoscape and STRING, and GO and KEGG enrichment analysis of target genes using Meatscape. The results showed that the low and high dose groups significantly reduced the number of coughs in mice (P<0.001) and decreased the levels of three inflammatory factors, tumor necrosis factor, interleukin 1α and interleukin 1β in mice serum, with significant differences in the high dose group (P<0.05, P<0.01, P<0.05). The results of the network pharmacological analysis showed that this compound could act on 78 targets including transcription factor AP-1, interleukin 6 and tumor necrosis factor, regulating 575 GO entries and 272 signalling pathways to exert cough suppressant effects, of which cordycepic acid, cordycepin, apigenin, beta-sitosterol and naringenin were the important material basis. The results indicated that the combination of Citri grandis Exocarpium-Cordyceps militaris has cough suppressant activity, which was the result of the combined action of multiple substances in the combination on multiple targets and pathways.http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100261citri grandis exocarpiumcordyceps militariscoughnetwork pharmacology |
| spellingShingle | Chunwei JIAO Jing ZHONG Haiyong HUANG Huijia LIANG Jiaoji ZHENG Jiaming CHEN Yizhen XIE Activity and Mechanism of Citri grandis Exocarpium-Cordyceps militaris Compound on Reliving Cough Based on Network Pharmacology Shipin gongye ke-ji citri grandis exocarpium cordyceps militaris cough network pharmacology |
| title | Activity and Mechanism of Citri grandis Exocarpium-Cordyceps militaris Compound on Reliving Cough Based on Network Pharmacology |
| title_full | Activity and Mechanism of Citri grandis Exocarpium-Cordyceps militaris Compound on Reliving Cough Based on Network Pharmacology |
| title_fullStr | Activity and Mechanism of Citri grandis Exocarpium-Cordyceps militaris Compound on Reliving Cough Based on Network Pharmacology |
| title_full_unstemmed | Activity and Mechanism of Citri grandis Exocarpium-Cordyceps militaris Compound on Reliving Cough Based on Network Pharmacology |
| title_short | Activity and Mechanism of Citri grandis Exocarpium-Cordyceps militaris Compound on Reliving Cough Based on Network Pharmacology |
| title_sort | activity and mechanism of citri grandis exocarpium cordyceps militaris compound on reliving cough based on network pharmacology |
| topic | citri grandis exocarpium cordyceps militaris cough network pharmacology |
| url | http://www.spgykj.com/cn/article/doi/10.13386/j.issn1002-0306.2021100261 |
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