Panduratin A Inhibits the Growth of A549 Cells through Induction of Apoptosis and Inhibition of NF-KappaB Translocation
In the present study we investigated the effects of panduratin A, isolated from Boesenbergia rotunda, on proliferation and apoptosis in A549 human non-small cell lung cancer cells. Cell proliferation and induction of apoptosis was determined by the real-time cellular analyzer (RTCA), MTT assay and H...
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| Format: | Article |
| Language: | English |
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MDPI AG
2011-03-01
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| Series: | Molecules |
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| Online Access: | http://www.mdpi.com/1420-3049/16/3/2583/ |
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| author | May-Lynn Lam Mohd. Rais Mustafa A. Hamid A. Hadi David R. Appleton Sui-Ting Lee Shiau-Chuen Cheah |
| author_facet | May-Lynn Lam Mohd. Rais Mustafa A. Hamid A. Hadi David R. Appleton Sui-Ting Lee Shiau-Chuen Cheah |
| author_sort | May-Lynn Lam |
| collection | DOAJ |
| description | In the present study we investigated the effects of panduratin A, isolated from Boesenbergia rotunda, on proliferation and apoptosis in A549 human non-small cell lung cancer cells. Cell proliferation and induction of apoptosis was determined by the real-time cellular analyzer (RTCA), MTT assay and High Content Screening (HCS). The RTCA assay indicated that panduratin A exhibited cytotoxicity, with an IC50 value of 4.4 µg/mL (10.8 µM). Panduratin A arrested cancer cells labeled with bromodeoxyuridine (BrdU) and phospho-Histone H3 in the mitotic phase. The cytotoxic effects of panduratin A were found to be accompanied by a dose-dependent induction of apoptosis, as assessed by DNA condensation, nuclear morphology and intensity, cell permeability, mitochondrial mass/ potential, F-actin and cytochrome c. In addition, treatment with an apoptosis-inducing concentration of panduratin A resulted in significant inhibition of Nuclear Factor-kappa Beta (NF-κB) translocation from cytoplasm to nuclei activated by tumor necrosis factor-alpha (TNF-α), as illustrated by the HCS assay. Our study provides evidence for cell growth inhibition and induction of apoptosis by panduratin A in the A549 cell line, suggesting its therapeutic potential as an NF-κB inhibitor. |
| first_indexed | 2024-12-19T02:49:04Z |
| format | Article |
| id | doaj.art-4bb71dc13ebf4c7a8b3a2755b5a8a45f |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-12-19T02:49:04Z |
| publishDate | 2011-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4bb71dc13ebf4c7a8b3a2755b5a8a45f2022-12-21T20:38:45ZengMDPI AGMolecules1420-30492011-03-011632583259810.3390/molecules16032583Panduratin A Inhibits the Growth of A549 Cells through Induction of Apoptosis and Inhibition of NF-KappaB TranslocationMay-Lynn LamMohd. Rais MustafaA. Hamid A. HadiDavid R. AppletonSui-Ting LeeShiau-Chuen CheahIn the present study we investigated the effects of panduratin A, isolated from Boesenbergia rotunda, on proliferation and apoptosis in A549 human non-small cell lung cancer cells. Cell proliferation and induction of apoptosis was determined by the real-time cellular analyzer (RTCA), MTT assay and High Content Screening (HCS). The RTCA assay indicated that panduratin A exhibited cytotoxicity, with an IC50 value of 4.4 µg/mL (10.8 µM). Panduratin A arrested cancer cells labeled with bromodeoxyuridine (BrdU) and phospho-Histone H3 in the mitotic phase. The cytotoxic effects of panduratin A were found to be accompanied by a dose-dependent induction of apoptosis, as assessed by DNA condensation, nuclear morphology and intensity, cell permeability, mitochondrial mass/ potential, F-actin and cytochrome c. In addition, treatment with an apoptosis-inducing concentration of panduratin A resulted in significant inhibition of Nuclear Factor-kappa Beta (NF-κB) translocation from cytoplasm to nuclei activated by tumor necrosis factor-alpha (TNF-α), as illustrated by the HCS assay. Our study provides evidence for cell growth inhibition and induction of apoptosis by panduratin A in the A549 cell line, suggesting its therapeutic potential as an NF-κB inhibitor.http://www.mdpi.com/1420-3049/16/3/2583/Panduratin AapoptosisHigh Content ScreeningReal-time Cellular AnalyzerNF-κB |
| spellingShingle | May-Lynn Lam Mohd. Rais Mustafa A. Hamid A. Hadi David R. Appleton Sui-Ting Lee Shiau-Chuen Cheah Panduratin A Inhibits the Growth of A549 Cells through Induction of Apoptosis and Inhibition of NF-KappaB Translocation Molecules Panduratin A apoptosis High Content Screening Real-time Cellular Analyzer NF-κB |
| title | Panduratin A Inhibits the Growth of A549 Cells through Induction of Apoptosis and Inhibition of NF-KappaB Translocation |
| title_full | Panduratin A Inhibits the Growth of A549 Cells through Induction of Apoptosis and Inhibition of NF-KappaB Translocation |
| title_fullStr | Panduratin A Inhibits the Growth of A549 Cells through Induction of Apoptosis and Inhibition of NF-KappaB Translocation |
| title_full_unstemmed | Panduratin A Inhibits the Growth of A549 Cells through Induction of Apoptosis and Inhibition of NF-KappaB Translocation |
| title_short | Panduratin A Inhibits the Growth of A549 Cells through Induction of Apoptosis and Inhibition of NF-KappaB Translocation |
| title_sort | panduratin a inhibits the growth of a549 cells through induction of apoptosis and inhibition of nf kappab translocation |
| topic | Panduratin A apoptosis High Content Screening Real-time Cellular Analyzer NF-κB |
| url | http://www.mdpi.com/1420-3049/16/3/2583/ |
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