Characterization of superoxide dismutase in the rumen bacteriumStreptococcus bovis
Superoxide dismutase (SOD) isoenzymes of the rumen bacterium Streptococcus bovis 4/1 were studied. Native PAGE showed a single band of Mn-SOD, unaffected by 10 mM cyanide or 5 mM hydrogen peroxide under both aerobic and anaerobic growth conditions. When the metals were removed from the growth medium...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2002-02-01
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| Series: | Veterinární Medicína |
| Subjects: | |
| Online Access: | https://vetmed.agriculturejournals.cz/artkey/vet-200202-0002_characterization-of-superoxide-dismutase-in-the-rumen-bacteriumstreptococcus-bovis.php |
| _version_ | 1828006916836556800 |
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| author | K. Holovská V. Lenártová K. Holovská P. Javorský |
| author_facet | K. Holovská V. Lenártová K. Holovská P. Javorský |
| author_sort | K. Holovská |
| collection | DOAJ |
| description | Superoxide dismutase (SOD) isoenzymes of the rumen bacterium Streptococcus bovis 4/1 were studied. Native PAGE showed a single band of Mn-SOD, unaffected by 10 mM cyanide or 5 mM hydrogen peroxide under both aerobic and anaerobic growth conditions. When the metals were removed from the growth medium by Chelex 100, the addition of manganese increased enzymatic activity, while addition of iron inhibited SOD activity. Changes in Mn-SOD and glutathione peroxidase (GSHPx) activities evoked by paraquat and increased values of TBARS indicated that these enzymes were not able to sufficiently prevent oxidative stress at given paraquat concentrations. |
| first_indexed | 2024-04-10T08:02:50Z |
| format | Article |
| id | doaj.art-4bb7e7eccc164883a7ba039d40a791f6 |
| institution | Directory Open Access Journal |
| issn | 0375-8427 1805-9392 |
| language | English |
| last_indexed | 2024-04-10T08:02:50Z |
| publishDate | 2002-02-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Veterinární Medicína |
| spelling | doaj.art-4bb7e7eccc164883a7ba039d40a791f62023-02-23T03:48:59ZengCzech Academy of Agricultural SciencesVeterinární Medicína0375-84271805-93922002-02-01472-3384410.17221/5801-VETMEDvet-200202-0002Characterization of superoxide dismutase in the rumen bacteriumStreptococcus bovisK. Holovská0V. Lenártová1K. Holovská2P. Javorský3Institute of Animal Physiology, Slovak Academy of Science, Košice, Slovak RepublicInstitute of Animal Physiology, Slovak Academy of Science, Košice, Slovak RepublicInstitute of Animal Physiology, Slovak Academy of Science, Košice, Slovak RepublicInstitute of Animal Physiology, Slovak Academy of Science, Košice, Slovak RepublicSuperoxide dismutase (SOD) isoenzymes of the rumen bacterium Streptococcus bovis 4/1 were studied. Native PAGE showed a single band of Mn-SOD, unaffected by 10 mM cyanide or 5 mM hydrogen peroxide under both aerobic and anaerobic growth conditions. When the metals were removed from the growth medium by Chelex 100, the addition of manganese increased enzymatic activity, while addition of iron inhibited SOD activity. Changes in Mn-SOD and glutathione peroxidase (GSHPx) activities evoked by paraquat and increased values of TBARS indicated that these enzymes were not able to sufficiently prevent oxidative stress at given paraquat concentrations.https://vetmed.agriculturejournals.cz/artkey/vet-200202-0002_characterization-of-superoxide-dismutase-in-the-rumen-bacteriumstreptococcus-bovis.phpstreptococcus bovisparaquatsuperoxide dismutaseglutathione peroxidase |
| spellingShingle | K. Holovská V. Lenártová K. Holovská P. Javorský Characterization of superoxide dismutase in the rumen bacteriumStreptococcus bovis Veterinární Medicína streptococcus bovis paraquat superoxide dismutase glutathione peroxidase |
| title | Characterization of superoxide dismutase in the rumen bacteriumStreptococcus bovis |
| title_full | Characterization of superoxide dismutase in the rumen bacteriumStreptococcus bovis |
| title_fullStr | Characterization of superoxide dismutase in the rumen bacteriumStreptococcus bovis |
| title_full_unstemmed | Characterization of superoxide dismutase in the rumen bacteriumStreptococcus bovis |
| title_short | Characterization of superoxide dismutase in the rumen bacteriumStreptococcus bovis |
| title_sort | characterization of superoxide dismutase in the rumen bacteriumstreptococcus bovis |
| topic | streptococcus bovis paraquat superoxide dismutase glutathione peroxidase |
| url | https://vetmed.agriculturejournals.cz/artkey/vet-200202-0002_characterization-of-superoxide-dismutase-in-the-rumen-bacteriumstreptococcus-bovis.php |
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