CRISPR-based biosensing is prospective for rapid and sensitive diagnosis of pediatric tuberculosis
Pediatric tuberculosis (TB) is an important part of global TB prevention and control. Diagnosis of childhood TB still remains challenging when using conventional tests, due to the non-specific clinical manifestations and paucibacillary nature of the specimens. Thus, a sensitive, rapid and low-cost d...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | International Journal of Infectious Diseases |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1201971220321445 |
| _version_ | 1818922303403065344 |
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| author | Chunyang Lyu Hua Shi Yali Cui Mingyuan Li Ziyi Yan Lingyi Yan Yongmei Jiang |
| author_facet | Chunyang Lyu Hua Shi Yali Cui Mingyuan Li Ziyi Yan Lingyi Yan Yongmei Jiang |
| author_sort | Chunyang Lyu |
| collection | DOAJ |
| description | Pediatric tuberculosis (TB) is an important part of global TB prevention and control. Diagnosis of childhood TB still remains challenging when using conventional tests, due to the non-specific clinical manifestations and paucibacillary nature of the specimens. Thus, a sensitive, rapid and low-cost diagnostic test is of great demand. Benefiting from specific and rapid Cas-protein-based catalytic activities, CRISPR-based biosensing platforms (CRISPR platforms) are showing superiority in detecting pathogen nucleic acid traces in clinical samples. Based on their excellent sensitivity, and time and cost saved in existing research, this study aimed to highlight the potential of CRISPR platforms as a tool for diagnosing pediatric TB, and advocate for studies to evaluate its performance in specimens collected from children, especially noninvasive specimens. These platforms are also promising in identifying drug resistance and genotyping. All of the above will help early diagnosis of pediatric TB, thus guide reasonable treatment, and be significant in achieving the World Health Organization End-TB strategy. |
| first_indexed | 2024-12-20T01:51:24Z |
| format | Article |
| id | doaj.art-4bb856112eb34d73a2e1751223f1f2e8 |
| institution | Directory Open Access Journal |
| issn | 1201-9712 |
| language | English |
| last_indexed | 2024-12-20T01:51:24Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | International Journal of Infectious Diseases |
| spelling | doaj.art-4bb856112eb34d73a2e1751223f1f2e82022-12-21T19:57:38ZengElsevierInternational Journal of Infectious Diseases1201-97122020-12-01101183187CRISPR-based biosensing is prospective for rapid and sensitive diagnosis of pediatric tuberculosisChunyang Lyu0Hua Shi1Yali Cui2Mingyuan Li3Ziyi Yan4Lingyi Yan5Yongmei Jiang6Department of Laboratory Medicine, West China Second Hospital, Sichuan University, Chengdu, Sichuan, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, ChinaDepartment of Laboratory Medicine, West China Second Hospital, Sichuan University, Chengdu, Sichuan, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, ChinaDepartment of Laboratory Medicine, West China Second Hospital, Sichuan University, Chengdu, Sichuan, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, ChinaDepartment of Laboratory Medicine, West China Second Hospital, Sichuan University, Chengdu, Sichuan, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, ChinaDepartment of Laboratory Medicine, West China Second Hospital, Sichuan University, Chengdu, Sichuan, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, ChinaDepartment of Laboratory Medicine, West China Second Hospital, Sichuan University, Chengdu, Sichuan, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, ChinaDepartment of Laboratory Medicine, West China Second Hospital, Sichuan University, Chengdu, Sichuan, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, Chengdu, Sichuan, China; Corresponding author at: Department of Laboratory Medicine, West China Second Hospital, Sichuan University, Chengdu, Sichuan, China.Pediatric tuberculosis (TB) is an important part of global TB prevention and control. Diagnosis of childhood TB still remains challenging when using conventional tests, due to the non-specific clinical manifestations and paucibacillary nature of the specimens. Thus, a sensitive, rapid and low-cost diagnostic test is of great demand. Benefiting from specific and rapid Cas-protein-based catalytic activities, CRISPR-based biosensing platforms (CRISPR platforms) are showing superiority in detecting pathogen nucleic acid traces in clinical samples. Based on their excellent sensitivity, and time and cost saved in existing research, this study aimed to highlight the potential of CRISPR platforms as a tool for diagnosing pediatric TB, and advocate for studies to evaluate its performance in specimens collected from children, especially noninvasive specimens. These platforms are also promising in identifying drug resistance and genotyping. All of the above will help early diagnosis of pediatric TB, thus guide reasonable treatment, and be significant in achieving the World Health Organization End-TB strategy.http://www.sciencedirect.com/science/article/pii/S1201971220321445Childhood tuberculosisDiagnosisCRISPR-CasNucleic acid detectionBiosensing platforms |
| spellingShingle | Chunyang Lyu Hua Shi Yali Cui Mingyuan Li Ziyi Yan Lingyi Yan Yongmei Jiang CRISPR-based biosensing is prospective for rapid and sensitive diagnosis of pediatric tuberculosis International Journal of Infectious Diseases Childhood tuberculosis Diagnosis CRISPR-Cas Nucleic acid detection Biosensing platforms |
| title | CRISPR-based biosensing is prospective for rapid and sensitive diagnosis of pediatric tuberculosis |
| title_full | CRISPR-based biosensing is prospective for rapid and sensitive diagnosis of pediatric tuberculosis |
| title_fullStr | CRISPR-based biosensing is prospective for rapid and sensitive diagnosis of pediatric tuberculosis |
| title_full_unstemmed | CRISPR-based biosensing is prospective for rapid and sensitive diagnosis of pediatric tuberculosis |
| title_short | CRISPR-based biosensing is prospective for rapid and sensitive diagnosis of pediatric tuberculosis |
| title_sort | crispr based biosensing is prospective for rapid and sensitive diagnosis of pediatric tuberculosis |
| topic | Childhood tuberculosis Diagnosis CRISPR-Cas Nucleic acid detection Biosensing platforms |
| url | http://www.sciencedirect.com/science/article/pii/S1201971220321445 |
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