Identification of Plant Viruses Infecting Pear Using RNA Sequencing
Asian pear (Pyrus pyrifolia) is a widely cultivated and commercially important fruit crop, which is occasionally subject to severe economic losses due to latent viral infections. Thus, the aim of the present study was to examine and provide a comprehensive overview of virus populations infecting a m...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Hanrimwon Publishing Company
2021-06-01
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| Series: | The Plant Pathology Journal |
| Subjects: | |
| Online Access: | http://www.ppjonline.org/upload/pdf/PPJ-OA-01-2021-0009.pdf |
| _version_ | 1811308493875970048 |
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| author | Nam-Yeon Kim Hyo-Jeong Lee Hong-Sup Kim Su-Heon Lee Jae-Sun Moon Rae-Dong Jeong |
| author_facet | Nam-Yeon Kim Hyo-Jeong Lee Hong-Sup Kim Su-Heon Lee Jae-Sun Moon Rae-Dong Jeong |
| author_sort | Nam-Yeon Kim |
| collection | DOAJ |
| description | Asian pear (Pyrus pyrifolia) is a widely cultivated and commercially important fruit crop, which is occasionally subject to severe economic losses due to latent viral infections. Thus, the aim of the present study was to examine and provide a comprehensive overview of virus populations infecting a major pear cultivar (‘Singo’) in Korea. From June 2017 to October 2019, leaf samples (n = 110) of pear trees from 35 orchards in five major pear-producing regions were collected and subjected to RNA sequencing. Most virus-associated contigs matched the sequences of known viruses, including apple stem grooving virus (ASGV) and apple stem pitting virus (ASPV). However, some contigs matched the sequences of apple green crinkle-associated virus and cucumber mosaic virus. In addition, three complete or nearly complete genomes were constructed based on transcriptome data and subjected to phylogenetic analyses. Based on the number of virus-associated reads, ASGV and ASPV were identified as the dominant viruses of ‘Singo.’ The present study describes the virome of a major pear cultivar in Korea, and looks into the diversity of viral communities in this cultivar. This study can provide valuable information on the complexity of genetic variability of viruses infecting pear trees. |
| first_indexed | 2024-04-13T09:24:15Z |
| format | Article |
| id | doaj.art-4bb85ab326da480a90e228b99134367b |
| institution | Directory Open Access Journal |
| issn | 1598-2254 2093-9280 |
| language | English |
| last_indexed | 2024-04-13T09:24:15Z |
| publishDate | 2021-06-01 |
| publisher | Hanrimwon Publishing Company |
| record_format | Article |
| series | The Plant Pathology Journal |
| spelling | doaj.art-4bb85ab326da480a90e228b99134367b2022-12-22T02:52:30ZengHanrimwon Publishing CompanyThe Plant Pathology Journal1598-22542093-92802021-06-0137325826710.5423/PPJ.OA.01.2021.00092250Identification of Plant Viruses Infecting Pear Using RNA SequencingNam-Yeon Kim0Hyo-Jeong Lee1Hong-Sup Kim2Su-Heon Lee3Jae-Sun Moon4Rae-Dong Jeong5 Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University, Gwangju 61185, Korea Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University, Gwangju 61185, Korea Seed Testing & Research Center, Korea Seed & Variety Service, Gimcheon 39660, Korea School of Applied Biosciences, Kyungpook National University, Daegu 98411, Korea Plant Genome Research Center, Korea Research Institute of Biosciences & Biotechnology, Daejeon 34141, Korea Department of Applied Biology, Institute of Environmentally Friendly Agriculture, Chonnam National University, Gwangju 61185, KoreaAsian pear (Pyrus pyrifolia) is a widely cultivated and commercially important fruit crop, which is occasionally subject to severe economic losses due to latent viral infections. Thus, the aim of the present study was to examine and provide a comprehensive overview of virus populations infecting a major pear cultivar (‘Singo’) in Korea. From June 2017 to October 2019, leaf samples (n = 110) of pear trees from 35 orchards in five major pear-producing regions were collected and subjected to RNA sequencing. Most virus-associated contigs matched the sequences of known viruses, including apple stem grooving virus (ASGV) and apple stem pitting virus (ASPV). However, some contigs matched the sequences of apple green crinkle-associated virus and cucumber mosaic virus. In addition, three complete or nearly complete genomes were constructed based on transcriptome data and subjected to phylogenetic analyses. Based on the number of virus-associated reads, ASGV and ASPV were identified as the dominant viruses of ‘Singo.’ The present study describes the virome of a major pear cultivar in Korea, and looks into the diversity of viral communities in this cultivar. This study can provide valuable information on the complexity of genetic variability of viruses infecting pear trees.http://www.ppjonline.org/upload/pdf/PPJ-OA-01-2021-0009.pdfpearplant virusesrna sequencingviral population |
| spellingShingle | Nam-Yeon Kim Hyo-Jeong Lee Hong-Sup Kim Su-Heon Lee Jae-Sun Moon Rae-Dong Jeong Identification of Plant Viruses Infecting Pear Using RNA Sequencing The Plant Pathology Journal pear plant viruses rna sequencing viral population |
| title | Identification of Plant Viruses Infecting Pear Using RNA Sequencing |
| title_full | Identification of Plant Viruses Infecting Pear Using RNA Sequencing |
| title_fullStr | Identification of Plant Viruses Infecting Pear Using RNA Sequencing |
| title_full_unstemmed | Identification of Plant Viruses Infecting Pear Using RNA Sequencing |
| title_short | Identification of Plant Viruses Infecting Pear Using RNA Sequencing |
| title_sort | identification of plant viruses infecting pear using rna sequencing |
| topic | pear plant viruses rna sequencing viral population |
| url | http://www.ppjonline.org/upload/pdf/PPJ-OA-01-2021-0009.pdf |
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