Microsatellite Primers for the Pacific Northwest Conifer <i>Callitropsis nootkatensis</i> (Cupressaceae)
Premise of the study: Microsatellite primers were developed for Nootka cypress (<i>Callitropsis nootkatensis</i>) to provide quantitative measures for gene conservation that can assist in guiding management decisions for a species experiencing climate-induced decline. Methods and Result...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-09-01
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| Series: | Applications in Plant Sciences |
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| Online Access: | http://www.bioone.org/doi/full/10.3732/apps.1300025 |
| _version_ | 1818042697380790272 |
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| author | Tara N. Jennings Brian J. Knaus Katherine Alderman Paul E. Hennon David V. D'Amore Richard Cronn |
| author_facet | Tara N. Jennings Brian J. Knaus Katherine Alderman Paul E. Hennon David V. D'Amore Richard Cronn |
| author_sort | Tara N. Jennings |
| collection | DOAJ |
| description | Premise of the study: Microsatellite primers were developed for Nootka cypress (<i>Callitropsis nootkatensis</i>) to provide quantitative measures for gene conservation that can assist in guiding management decisions for a species experiencing climate-induced decline.
Methods and Results: Using multiplexed massively parallel sequencing, we identified 136,785 microsatellite-containing sequences from 489,625 Illumina paired-end 80-bp reads. After stringent filtering, we selected 144 primer pairs and screened variation at these loci in five populations of <i>C. nootkatensis</i>. Loci show between three and 36 dinucleotide repeats per locus, with an average of 13. Screening of these markers in the Pacific Northwest relative <i>Chamaecyparis lawsoniana</i> demonstrated no marker transferability. This finding highlights the narrow taxonomic utility of microsatellite markers in <i>Callitropsis</i>.
Conclusions: These microsatellites show high polymorphism and can be used for routine screening of natural variation in <i>Callitropsis nootkatensis</i>, and will be particularly helpful in identifying clones and inbred relatives at the stand-level. |
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| institution | Directory Open Access Journal |
| issn | 2168-0450 |
| language | English |
| last_indexed | 2024-12-10T08:50:26Z |
| publishDate | 2013-09-01 |
| publisher | Wiley |
| record_format | Article |
| series | Applications in Plant Sciences |
| spelling | doaj.art-4bbbf5b0f9b343c48b22d851465c17a02022-12-22T01:55:36ZengWileyApplications in Plant Sciences2168-04502013-09-0119130002510.3732/apps.1300025Microsatellite Primers for the Pacific Northwest Conifer <i>Callitropsis nootkatensis</i> (Cupressaceae)Tara N. Jennings0Brian J. Knaus1Katherine Alderman2Paul E. Hennon3David V. D'Amore4Richard Cronn5Pacific Northwest Research Station, USDA Forest Service, 3200 SW Jefferson Way, Corvallis, Oregon 97331 USAPacific Northwest Research Station, USDA Forest Service, 3200 SW Jefferson Way, Corvallis, Oregon 97331 USA; Horticultural Crops Research Unit, USDA Agricultural Research Service, 3240 NW Orchard Avenue, Oregon 97331 USAPacific Northwest Research Station, USDA Forest Service, 3200 SW Jefferson Way, Corvallis, Oregon 97331 USAPacific Northwest Research Station, USDA Forest Service, 11305 Glacier Highway, Juneau, Alaska 99801 USAPacific Northwest Research Station, USDA Forest Service, 11305 Glacier Highway, Juneau, Alaska 99801 USAPacific Northwest Research Station, USDA Forest Service, 3200 SW Jefferson Way, Corvallis, Oregon 97331 USAPremise of the study: Microsatellite primers were developed for Nootka cypress (<i>Callitropsis nootkatensis</i>) to provide quantitative measures for gene conservation that can assist in guiding management decisions for a species experiencing climate-induced decline. Methods and Results: Using multiplexed massively parallel sequencing, we identified 136,785 microsatellite-containing sequences from 489,625 Illumina paired-end 80-bp reads. After stringent filtering, we selected 144 primer pairs and screened variation at these loci in five populations of <i>C. nootkatensis</i>. Loci show between three and 36 dinucleotide repeats per locus, with an average of 13. Screening of these markers in the Pacific Northwest relative <i>Chamaecyparis lawsoniana</i> demonstrated no marker transferability. This finding highlights the narrow taxonomic utility of microsatellite markers in <i>Callitropsis</i>. Conclusions: These microsatellites show high polymorphism and can be used for routine screening of natural variation in <i>Callitropsis nootkatensis</i>, and will be particularly helpful in identifying clones and inbred relatives at the stand-level.http://www.bioone.org/doi/full/10.3732/apps.1300025Callitropsis nootkatensisChamaecyparis nootkatensisclimate changegermplasmPacific NorthwestNootka cypress |
| spellingShingle | Tara N. Jennings Brian J. Knaus Katherine Alderman Paul E. Hennon David V. D'Amore Richard Cronn Microsatellite Primers for the Pacific Northwest Conifer <i>Callitropsis nootkatensis</i> (Cupressaceae) Applications in Plant Sciences Callitropsis nootkatensis Chamaecyparis nootkatensis climate change germplasm Pacific Northwest Nootka cypress |
| title | Microsatellite Primers for the Pacific Northwest Conifer <i>Callitropsis nootkatensis</i> (Cupressaceae) |
| title_full | Microsatellite Primers for the Pacific Northwest Conifer <i>Callitropsis nootkatensis</i> (Cupressaceae) |
| title_fullStr | Microsatellite Primers for the Pacific Northwest Conifer <i>Callitropsis nootkatensis</i> (Cupressaceae) |
| title_full_unstemmed | Microsatellite Primers for the Pacific Northwest Conifer <i>Callitropsis nootkatensis</i> (Cupressaceae) |
| title_short | Microsatellite Primers for the Pacific Northwest Conifer <i>Callitropsis nootkatensis</i> (Cupressaceae) |
| title_sort | microsatellite primers for the pacific northwest conifer i callitropsis nootkatensis i cupressaceae |
| topic | Callitropsis nootkatensis Chamaecyparis nootkatensis climate change germplasm Pacific Northwest Nootka cypress |
| url | http://www.bioone.org/doi/full/10.3732/apps.1300025 |
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