Grooved poly(lactide-co-trimethylene carbonate) substrates in tenogenic media maintain human tendon derived cell phenotype in culture – A preliminary report✰
Tissue engineering strategies for tendon repair and regeneration rely heavily on the use of tendon derived cells. However, these cells frequently undergo phenotypic drift in vitro, which compromises their therapeutic potential. In order to maintain the phenotype of tendon derived cells in vitro, mic...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Biomedical Engineering Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667099223000282 |
| _version_ | 1797398410195632128 |
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| author | Sofia Ribeiro Eugenia Pugliese Stefanie H. Korntner Emanuel M. Fernandes Manuela E. Gomes Rui L. Reis Alan O'Riordan Stephen Kearns Jack L. Kelly Manus Biggs Yves Bayon Dimitrios I. Zeugolis |
| author_facet | Sofia Ribeiro Eugenia Pugliese Stefanie H. Korntner Emanuel M. Fernandes Manuela E. Gomes Rui L. Reis Alan O'Riordan Stephen Kearns Jack L. Kelly Manus Biggs Yves Bayon Dimitrios I. Zeugolis |
| author_sort | Sofia Ribeiro |
| collection | DOAJ |
| description | Tissue engineering strategies for tendon repair and regeneration rely heavily on the use of tendon derived cells. However, these cells frequently undergo phenotypic drift in vitro, which compromises their therapeutic potential. In order to maintain the phenotype of tendon derived cells in vitro, microenvironmental cues (biophysical, biochemical and/or biological in origin) have been used to better imitate the complex tendon microenvironment. Herein, the influence of planar and grooved (groove width of ∼1.0 µm, groove depth of ∼1.4 µm and distance between groves of ∼1.7 µm) poly(glycolide-co-ε-caprolactone) substrates with elastic modulus of 7 kPa and poly(lactide-co-trimethylene carbonate) substrates with elastic modulus of 12 kPa on human tendon derived cell response was assessed, using planar tissue culture plastic substrates of 3 GPa elastic modulus as control, in both basal and tenogenic media. At day 17, the grooved 12 kPa poly(lactide-co-trimethylene carbonate) substrate induced the highest deposition and alignment of collagen type I in tenogenic media. At day 17, the grooved 12 kPa poly(lactide-co-trimethylene carbonate) substrate and the tissue culture plastic induced the highest deposition and the tissue culture plastic and the planar 7 kPa poly(glycolide-co-ε-caprolactone) induced the lowest alignment of tenascin C in tenogenic media. Also at day 17 in tenogenic media, the grooved 12 kPa poly(lactide-co-trimethylene carbonate) substrate induced the upregulation of most tenogenic genes (COL1A1, COL3A1, MKX, TNMD). Our data further support the notion of multifactorial tissue engineering for effective control over cell fate in vitro setting. |
| first_indexed | 2024-03-09T01:25:15Z |
| format | Article |
| id | doaj.art-4bbc8c49c86e4384b796d34f1db7d478 |
| institution | Directory Open Access Journal |
| issn | 2667-0992 |
| language | English |
| last_indexed | 2024-03-09T01:25:15Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biomedical Engineering Advances |
| spelling | doaj.art-4bbc8c49c86e4384b796d34f1db7d4782023-12-10T06:18:48ZengElsevierBiomedical Engineering Advances2667-09922023-11-016100098Grooved poly(lactide-co-trimethylene carbonate) substrates in tenogenic media maintain human tendon derived cell phenotype in culture – A preliminary report✰Sofia Ribeiro0Eugenia Pugliese1Stefanie H. Korntner2Emanuel M. Fernandes3Manuela E. Gomes4Rui L. Reis5Alan O'Riordan6Stephen Kearns7Jack L. Kelly8Manus Biggs9Yves Bayon10Dimitrios I. Zeugolis11Medtronic, Sofradim Production, Trevoux, France; Regenerative, Modular & Developmental Engineering Laboratory (REMODEL) University of Galway, Galway, Ireland; Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM) University of Galway, IrelandRegenerative, Modular & Developmental Engineering Laboratory (REMODEL) University of Galway, Galway, Ireland; Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM) University of Galway, IrelandRegenerative, Modular & Developmental Engineering Laboratory (REMODEL) University of Galway, Galway, Ireland; Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM) University of Galway, Ireland3B's Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's – PT Government Associate Laboratory, Braga, Guimarães, Portugal3B's Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's – PT Government Associate Laboratory, Braga, Guimarães, Portugal3B's Research Group, I3Bs – Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Guimarães, Portugal; ICVS/3B's – PT Government Associate Laboratory, Braga, Guimarães, PortugalTyndall National Institute, Cork, IrelandOrthopaedic Surgery, University Hospital Galway and Department of Medicine, University of Galway, Galway, IrelandPlastic and Reconstructive Surgery, University Hospital Galway and Department of Medicine, University of Galway, Galway, IrelandScience Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM) University of Galway, IrelandMedtronic, Sofradim Production, Trevoux, FranceRegenerative, Modular & Developmental Engineering Laboratory (REMODEL) University of Galway, Galway, Ireland; Science Foundation Ireland (SFI) Centre for Research in Medical Devices (CÚRAM) University of Galway, Ireland; Regenerative, Modular & Developmental Engineering Laboratory (REMODEL), Charles Institute of Dermatology, Conway Institute of Biomolecular and Biomedical Research and School of Mechanical and Materials Engineering, University College Dublin (UCD), Dublin, Ireland; Corresponding author.Tissue engineering strategies for tendon repair and regeneration rely heavily on the use of tendon derived cells. However, these cells frequently undergo phenotypic drift in vitro, which compromises their therapeutic potential. In order to maintain the phenotype of tendon derived cells in vitro, microenvironmental cues (biophysical, biochemical and/or biological in origin) have been used to better imitate the complex tendon microenvironment. Herein, the influence of planar and grooved (groove width of ∼1.0 µm, groove depth of ∼1.4 µm and distance between groves of ∼1.7 µm) poly(glycolide-co-ε-caprolactone) substrates with elastic modulus of 7 kPa and poly(lactide-co-trimethylene carbonate) substrates with elastic modulus of 12 kPa on human tendon derived cell response was assessed, using planar tissue culture plastic substrates of 3 GPa elastic modulus as control, in both basal and tenogenic media. At day 17, the grooved 12 kPa poly(lactide-co-trimethylene carbonate) substrate induced the highest deposition and alignment of collagen type I in tenogenic media. At day 17, the grooved 12 kPa poly(lactide-co-trimethylene carbonate) substrate and the tissue culture plastic induced the highest deposition and the tissue culture plastic and the planar 7 kPa poly(glycolide-co-ε-caprolactone) induced the lowest alignment of tenascin C in tenogenic media. Also at day 17 in tenogenic media, the grooved 12 kPa poly(lactide-co-trimethylene carbonate) substrate induced the upregulation of most tenogenic genes (COL1A1, COL3A1, MKX, TNMD). Our data further support the notion of multifactorial tissue engineering for effective control over cell fate in vitro setting.http://www.sciencedirect.com/science/article/pii/S2667099223000282Biodegradable polyestersSurface topographySubstrate rigidityTenogenic phenotype |
| spellingShingle | Sofia Ribeiro Eugenia Pugliese Stefanie H. Korntner Emanuel M. Fernandes Manuela E. Gomes Rui L. Reis Alan O'Riordan Stephen Kearns Jack L. Kelly Manus Biggs Yves Bayon Dimitrios I. Zeugolis Grooved poly(lactide-co-trimethylene carbonate) substrates in tenogenic media maintain human tendon derived cell phenotype in culture – A preliminary report✰ Biomedical Engineering Advances Biodegradable polyesters Surface topography Substrate rigidity Tenogenic phenotype |
| title | Grooved poly(lactide-co-trimethylene carbonate) substrates in tenogenic media maintain human tendon derived cell phenotype in culture – A preliminary report✰ |
| title_full | Grooved poly(lactide-co-trimethylene carbonate) substrates in tenogenic media maintain human tendon derived cell phenotype in culture – A preliminary report✰ |
| title_fullStr | Grooved poly(lactide-co-trimethylene carbonate) substrates in tenogenic media maintain human tendon derived cell phenotype in culture – A preliminary report✰ |
| title_full_unstemmed | Grooved poly(lactide-co-trimethylene carbonate) substrates in tenogenic media maintain human tendon derived cell phenotype in culture – A preliminary report✰ |
| title_short | Grooved poly(lactide-co-trimethylene carbonate) substrates in tenogenic media maintain human tendon derived cell phenotype in culture – A preliminary report✰ |
| title_sort | grooved poly lactide co trimethylene carbonate substrates in tenogenic media maintain human tendon derived cell phenotype in culture a preliminary report✰ |
| topic | Biodegradable polyesters Surface topography Substrate rigidity Tenogenic phenotype |
| url | http://www.sciencedirect.com/science/article/pii/S2667099223000282 |
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