Immunogenicity of enterotoxigenic Escherichia coli outer membrane vesicles encapsulated in chitosan nanoparticles
Objective(s): Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrheal disease in humans, particularly in children under 5 years and travelers in developing countries. To our knowledge, no vaccine is licensed yet to protect against ETEC infection. Like many Gram-negative pathogens,...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2018-03-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_10145_f5c77cfc2ec1b5202615bf10b05ef63f.pdf |
| _version_ | 1818235111337885696 |
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| author | Nafiseh Noroozi Seyed Latif Mousavi Gargari Shahram Nazarian Samaneh Sarvary Razieh Rezaei |
| author_facet | Nafiseh Noroozi Seyed Latif Mousavi Gargari Shahram Nazarian Samaneh Sarvary Razieh Rezaei |
| author_sort | Nafiseh Noroozi |
| collection | DOAJ |
| description | Objective(s): Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrheal disease in humans, particularly in children under 5 years and travelers in developing countries. To our knowledge, no vaccine is licensed yet to protect against ETEC infection. Like many Gram-negative pathogens, ETEC can secrete outer membrane vesicles (OMVs). These structures contain various immunogenic virulence proteins such as LT and therefore can be used as vaccine candidates. In this study we attempted to isolate the OMVs of ETEC cultivated at different temperatures and evaluate their immunogenicity and protective efficacy in a murine model of infection. Materials and Methods: OMVs was purified from bacterial supernatant by ultracentrifugation. OMVs were encapsulated in chitosan nanoparticles prepared by ionic gelation method within a layer of Eudragit L100 for oral delivery. Female BALB/c mice of 9 weeks’ old were immunized by parenteral injection and oral administration with free and encapsulated OMVs obtained from bacteria cultivated at 37°C and 42°C. The serum samples were collected and the antibody titers were measured by an enzyme-linked immunosorbent assay (ELISA). Results: The protein concentrations of OMVs were 3.47 mg/ml and 2.46 mg/ml for bacteria grown at 37°C and 42°C respectively. OMVs loaded into nanoparticles (NP-OMVs) were homogeneous and spherical in shape, with a size of 532 nm. The encapsulation efficiency of NP was 90%. Mice immunized with OMVs, inhibited the ETEC colonization in their small intestine and induced production of antibodies against LT toxin. Conclusion: The results obtained in this research place OMVs among promising candidates to be used for vaccination. |
| first_indexed | 2024-12-12T11:48:46Z |
| format | Article |
| id | doaj.art-4bbce0e48ddc490b82dce34b94867b8b |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-12T11:48:46Z |
| publishDate | 2018-03-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-4bbce0e48ddc490b82dce34b94867b8b2022-12-22T00:25:23ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742018-03-0121328429110.22038/ijbms.2018.25886.637110145Immunogenicity of enterotoxigenic Escherichia coli outer membrane vesicles encapsulated in chitosan nanoparticlesNafiseh Noroozi0Seyed Latif Mousavi Gargari1Shahram Nazarian2Samaneh Sarvary3Razieh Rezaei4Department of Biology, Faculty of Science, Shahed University, Tehran, IranDepartment of Biology, Faculty of Science, Shahed University, Tehran, IranDepartment of Biology, Faculty of Science, Imam Hossein University, Tehran, IranDepartment of Biology, Faculty of Science, Shahed University, Tehran, IranDepartment of Biology, Faculty of Science, Shahed University, Tehran, IranObjective(s): Enterotoxigenic Escherichia coli (ETEC) is an important cause of diarrheal disease in humans, particularly in children under 5 years and travelers in developing countries. To our knowledge, no vaccine is licensed yet to protect against ETEC infection. Like many Gram-negative pathogens, ETEC can secrete outer membrane vesicles (OMVs). These structures contain various immunogenic virulence proteins such as LT and therefore can be used as vaccine candidates. In this study we attempted to isolate the OMVs of ETEC cultivated at different temperatures and evaluate their immunogenicity and protective efficacy in a murine model of infection. Materials and Methods: OMVs was purified from bacterial supernatant by ultracentrifugation. OMVs were encapsulated in chitosan nanoparticles prepared by ionic gelation method within a layer of Eudragit L100 for oral delivery. Female BALB/c mice of 9 weeks’ old were immunized by parenteral injection and oral administration with free and encapsulated OMVs obtained from bacteria cultivated at 37°C and 42°C. The serum samples were collected and the antibody titers were measured by an enzyme-linked immunosorbent assay (ELISA). Results: The protein concentrations of OMVs were 3.47 mg/ml and 2.46 mg/ml for bacteria grown at 37°C and 42°C respectively. OMVs loaded into nanoparticles (NP-OMVs) were homogeneous and spherical in shape, with a size of 532 nm. The encapsulation efficiency of NP was 90%. Mice immunized with OMVs, inhibited the ETEC colonization in their small intestine and induced production of antibodies against LT toxin. Conclusion: The results obtained in this research place OMVs among promising candidates to be used for vaccination.http://ijbms.mums.ac.ir/article_10145_f5c77cfc2ec1b5202615bf10b05ef63f.pdfEnterotoxigenic Escherichia coliVirulence factorsOuter membrane vesicle Chitosan nanoparticle |
| spellingShingle | Nafiseh Noroozi Seyed Latif Mousavi Gargari Shahram Nazarian Samaneh Sarvary Razieh Rezaei Immunogenicity of enterotoxigenic Escherichia coli outer membrane vesicles encapsulated in chitosan nanoparticles Iranian Journal of Basic Medical Sciences Enterotoxigenic Escherichia coli Virulence factors Outer membrane vesicle Chitosan nanoparticle |
| title | Immunogenicity of enterotoxigenic Escherichia coli outer membrane vesicles encapsulated in chitosan nanoparticles |
| title_full | Immunogenicity of enterotoxigenic Escherichia coli outer membrane vesicles encapsulated in chitosan nanoparticles |
| title_fullStr | Immunogenicity of enterotoxigenic Escherichia coli outer membrane vesicles encapsulated in chitosan nanoparticles |
| title_full_unstemmed | Immunogenicity of enterotoxigenic Escherichia coli outer membrane vesicles encapsulated in chitosan nanoparticles |
| title_short | Immunogenicity of enterotoxigenic Escherichia coli outer membrane vesicles encapsulated in chitosan nanoparticles |
| title_sort | immunogenicity of enterotoxigenic escherichia coli outer membrane vesicles encapsulated in chitosan nanoparticles |
| topic | Enterotoxigenic Escherichia coli Virulence factors Outer membrane vesicle Chitosan nanoparticle |
| url | http://ijbms.mums.ac.ir/article_10145_f5c77cfc2ec1b5202615bf10b05ef63f.pdf |
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