Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress
Drought and heat stress, especially their combination, greatly affect crop production. Many studies have described transcriptome, proteome and phosphoproteome changes in response of plants to drought or heat stress. However, the study about the phosphoproteomic changes in response of crops to the co...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-05-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00298/full |
| _version_ | 1819064802770681856 |
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| author | Xiuli eHu Liuji eWu Feiyun eZhao Dayong eZhang Guohui eZhu Chaohai eLi Wei eWang |
| author_facet | Xiuli eHu Liuji eWu Feiyun eZhao Dayong eZhang Guohui eZhu Chaohai eLi Wei eWang |
| author_sort | Xiuli eHu |
| collection | DOAJ |
| description | Drought and heat stress, especially their combination, greatly affect crop production. Many studies have described transcriptome, proteome and phosphoproteome changes in response of plants to drought or heat stress. However, the study about the phosphoproteomic changes in response of crops to the combination stress is scare. To understand the mechanism of maize responses to the drought and heat combination stress, phosphoproteomic analysis was performed on maize leaves by using multiplex iTRAQ-based quantitative proteomic and LC-MS/MS methods. Five-leaf-stage maize was subjected to drought, heat or their combination, and the leaves were collected. Globally, heat, drought and the combined stress significantly changed the phosphorylation levels of 172, 149 and 144 phosphopeptides, respectively. These phosphopeptides corresponded to 282 proteins. Among them, 23 only responded to the combined stress and could not be predicted from their responses to single stressors; 30 and 75 only responded to drought and heat, respectively. Notably, 19 proteins were phosphorylated on different sites in response to the single and combination stresses. Of the seven significantly enriched phosphorylation motifs identified, two were common for all stresses, two were common for heat and the combined stress, and one was specific to the combined stress. The signaling pathways in which the phosphoproteins were involved clearly differed among the three stresses. Functional characterization of the phosphoproteins and the pathways identified here could lead to new targets for the enhancement of crop stress tolerance, which will be particularly important in the face of climate change and the increasing prevalence of abiotic stressors. |
| first_indexed | 2024-12-21T15:36:22Z |
| format | Article |
| id | doaj.art-4bbd44eb354349b3994134d05228bf66 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-21T15:36:22Z |
| publishDate | 2015-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-4bbd44eb354349b3994134d05228bf662022-12-21T18:58:38ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2015-05-01610.3389/fpls.2015.00298135320Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stressXiuli eHu0Liuji eWu1Feiyun eZhao2Dayong eZhang3Guohui eZhu4Chaohai eLi5Wei eWang6Henan Agricultural UniversityHenan Agricultural UniversityHenan Agricultural UniversityJiangsu Academy of Agricultural Sciences Institute of BiotechnologySouth China Agricultural UniversityHenan Agricultural UniversityHenan Agricultural UniversityDrought and heat stress, especially their combination, greatly affect crop production. Many studies have described transcriptome, proteome and phosphoproteome changes in response of plants to drought or heat stress. However, the study about the phosphoproteomic changes in response of crops to the combination stress is scare. To understand the mechanism of maize responses to the drought and heat combination stress, phosphoproteomic analysis was performed on maize leaves by using multiplex iTRAQ-based quantitative proteomic and LC-MS/MS methods. Five-leaf-stage maize was subjected to drought, heat or their combination, and the leaves were collected. Globally, heat, drought and the combined stress significantly changed the phosphorylation levels of 172, 149 and 144 phosphopeptides, respectively. These phosphopeptides corresponded to 282 proteins. Among them, 23 only responded to the combined stress and could not be predicted from their responses to single stressors; 30 and 75 only responded to drought and heat, respectively. Notably, 19 proteins were phosphorylated on different sites in response to the single and combination stresses. Of the seven significantly enriched phosphorylation motifs identified, two were common for all stresses, two were common for heat and the combined stress, and one was specific to the combined stress. The signaling pathways in which the phosphoproteins were involved clearly differed among the three stresses. Functional characterization of the phosphoproteins and the pathways identified here could lead to new targets for the enhancement of crop stress tolerance, which will be particularly important in the face of climate change and the increasing prevalence of abiotic stressors.http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00298/fullPhosphoproteinsMaizephosphoproteomicsDrought and heatiTRAQ labeling |
| spellingShingle | Xiuli eHu Liuji eWu Feiyun eZhao Dayong eZhang Guohui eZhu Chaohai eLi Wei eWang Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress Frontiers in Plant Science Phosphoproteins Maize phosphoproteomics Drought and heat iTRAQ labeling |
| title | Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress |
| title_full | Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress |
| title_fullStr | Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress |
| title_full_unstemmed | Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress |
| title_short | Phosphoproteomic analysis of the response of maize leaves to drought, heat and their combination stress |
| title_sort | phosphoproteomic analysis of the response of maize leaves to drought heat and their combination stress |
| topic | Phosphoproteins Maize phosphoproteomics Drought and heat iTRAQ labeling |
| url | http://journal.frontiersin.org/Journal/10.3389/fpls.2015.00298/full |
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