Bartonella spp. Infections Identified by Molecular Methods, United States
Molecular methods can enable rapid identification of Bartonella spp. infections, which are difficult to diagnose by using culture or serology. We analyzed clinical test results of PCR that targeted bacterial 16S rRNA hypervariable V1–V2 regions only or in parallel with PCR of Bartonella-specific ri...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2023-03-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/29/3/22-1223_article |
| _version_ | 1797895681403256832 |
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| author | David W. McCormick Sara L. Rassoulian-Barrett Daniel R. Hoogestraat Stephen J. Salipante Dhruba SenGupta Elizabeth A. Dietrich Brad T. Cookson Grace E. Marx Joshua A. Lieberman |
| author_facet | David W. McCormick Sara L. Rassoulian-Barrett Daniel R. Hoogestraat Stephen J. Salipante Dhruba SenGupta Elizabeth A. Dietrich Brad T. Cookson Grace E. Marx Joshua A. Lieberman |
| author_sort | David W. McCormick |
| collection | DOAJ |
| description |
Molecular methods can enable rapid identification of Bartonella spp. infections, which are difficult to diagnose by using culture or serology. We analyzed clinical test results of PCR that targeted bacterial 16S rRNA hypervariable V1–V2 regions only or in parallel with PCR of Bartonella-specific ribC gene. We identified 430 clinical specimens infected with Bartonella spp. from 420 patients in the United States. Median patient age was 37 (range 1–79) years; 62% were male. We identified B. henselae in 77%, B. quintana in 13%, B. clarridgeiae in 1%, B. vinsonii in 1%, and B. washoensis in 1% of specimens. B. quintana was detected in 83% of cardiac specimens; B. henselae was detected in 34% of lymph node specimens. We detected novel or uncommon Bartonella spp. in 9 patients. Molecular diagnostic testing can identify Bartonella spp. infections, including uncommon and undescribed species, and might be particularly useful for patients who have culture-negative endocarditis or lymphadenitis.
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| first_indexed | 2024-04-10T07:29:41Z |
| format | Article |
| id | doaj.art-4bc11362389b40f793bbefd1e23d5a1f |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-04-10T07:29:41Z |
| publishDate | 2023-03-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-4bc11362389b40f793bbefd1e23d5a1f2023-02-23T18:06:30ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592023-03-0129346747610.3201/eid2903.221223Bartonella spp. Infections Identified by Molecular Methods, United StatesDavid W. McCormickSara L. Rassoulian-BarrettDaniel R. HoogestraatStephen J. SalipanteDhruba SenGuptaElizabeth A. DietrichBrad T. CooksonGrace E. MarxJoshua A. Lieberman Molecular methods can enable rapid identification of Bartonella spp. infections, which are difficult to diagnose by using culture or serology. We analyzed clinical test results of PCR that targeted bacterial 16S rRNA hypervariable V1–V2 regions only or in parallel with PCR of Bartonella-specific ribC gene. We identified 430 clinical specimens infected with Bartonella spp. from 420 patients in the United States. Median patient age was 37 (range 1–79) years; 62% were male. We identified B. henselae in 77%, B. quintana in 13%, B. clarridgeiae in 1%, B. vinsonii in 1%, and B. washoensis in 1% of specimens. B. quintana was detected in 83% of cardiac specimens; B. henselae was detected in 34% of lymph node specimens. We detected novel or uncommon Bartonella spp. in 9 patients. Molecular diagnostic testing can identify Bartonella spp. infections, including uncommon and undescribed species, and might be particularly useful for patients who have culture-negative endocarditis or lymphadenitis. https://wwwnc.cdc.gov/eid/article/29/3/22-1223_articleBartonellabacteriaparasitesvector-borne infectionszoonosesendocarditis |
| spellingShingle | David W. McCormick Sara L. Rassoulian-Barrett Daniel R. Hoogestraat Stephen J. Salipante Dhruba SenGupta Elizabeth A. Dietrich Brad T. Cookson Grace E. Marx Joshua A. Lieberman Bartonella spp. Infections Identified by Molecular Methods, United States Emerging Infectious Diseases Bartonella bacteria parasites vector-borne infections zoonoses endocarditis |
| title | Bartonella spp. Infections Identified by Molecular Methods, United States |
| title_full | Bartonella spp. Infections Identified by Molecular Methods, United States |
| title_fullStr | Bartonella spp. Infections Identified by Molecular Methods, United States |
| title_full_unstemmed | Bartonella spp. Infections Identified by Molecular Methods, United States |
| title_short | Bartonella spp. Infections Identified by Molecular Methods, United States |
| title_sort | bartonella spp infections identified by molecular methods united states |
| topic | Bartonella bacteria parasites vector-borne infections zoonoses endocarditis |
| url | https://wwwnc.cdc.gov/eid/article/29/3/22-1223_article |
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