Dataset of De Novo hybrid berry transcriptome profiling and characterization of Piper species (Piper nigrum and Piper longum) using Illumina and Nanopore sequencing
Piper nigrum and Piper longum are the most popular and economically essential spice crops globally valued for their aromatic alkaloids, especially Piperine. However, Piperine synthesis pathway mechanisms are not yet well known. This work was aimed to generate the full-length comparative berry transc...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-06-01
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| Series: | Data in Brief |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340922004632 |
| _version_ | 1818216968667267072 |
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| author | Johnson K. George M.A. Fayad S. Shelvy T.E. Sheeja Santhosh J. Eapen Sona Charles Anil Rai Dinesh Kumar |
| author_facet | Johnson K. George M.A. Fayad S. Shelvy T.E. Sheeja Santhosh J. Eapen Sona Charles Anil Rai Dinesh Kumar |
| author_sort | Johnson K. George |
| collection | DOAJ |
| description | Piper nigrum and Piper longum are the most popular and economically essential spice crops globally valued for their aromatic alkaloids, especially Piperine. However, Piperine synthesis pathway mechanisms are not yet well known. This work was aimed to generate the full-length comparative berry transcriptome analysis dataset of P. nigrum and P. longum by Illumina and Nanopore sequencing platforms. While short-read sequencing technology is widely using to capture transcriptome profiles, there are still some limitations due to the read length. We used Oxford Nanopore technology for long reads and the Illumina sequencing platform for short reads to generate a hybrid transcriptome assembly from half matured and fully matured berries of P. nigrum and P. longum. From P. nigrum and P. longum 37.3 million and 38.1 million raw reads were generated respectively. A total of 308369 contigs from P. nigrum and 267715 contigs from P. longum were obtained and successfully annotated. The transcriptome data revealed gene families involved in piperine and other secondary metabolite biosynthetic pathways. The raw data were uploaded to NCBI database. This dataset shed light on the further exploration of the piperine biosynthetic pathway, its transcriptomic changes, and evolution. Data generated has been submitted to SRA of NCBI with Bio samples accession: (SAMN13981803, SAMN22826456). |
| first_indexed | 2024-12-12T07:00:24Z |
| format | Article |
| id | doaj.art-4bc1235fd9994ecda9694edf7b562b12 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-12T07:00:24Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-4bc1235fd9994ecda9694edf7b562b122022-12-22T00:33:51ZengElsevierData in Brief2352-34092022-06-0142108261Dataset of De Novo hybrid berry transcriptome profiling and characterization of Piper species (Piper nigrum and Piper longum) using Illumina and Nanopore sequencingJohnson K. George0M.A. Fayad1S. Shelvy2T.E. Sheeja3Santhosh J. Eapen4Sona Charles5Anil Rai6Dinesh Kumar7ICAR- Indian Institute of Spices Research, Kozhikode, Kerala, 673 012, IndiaICAR- Indian Institute of Spices Research, Kozhikode, Kerala, 673 012, IndiaICAR- Indian Institute of Spices Research, Kozhikode, Kerala, 673 012, IndiaICAR- Indian Institute of Spices Research, Kozhikode, Kerala, 673 012, India; Corresponding author.ICAR- Indian Institute of Spices Research, Kozhikode, Kerala, 673 012, IndiaICAR- Indian Institute of Spices Research, Kozhikode, Kerala, 673 012, IndiaICAR – Indian Agricultural Statistical Research Institute, Library Ave, Pusa, New Delhi, Delhi, 110012, IndiaICAR – Indian Agricultural Statistical Research Institute, Library Ave, Pusa, New Delhi, Delhi, 110012, IndiaPiper nigrum and Piper longum are the most popular and economically essential spice crops globally valued for their aromatic alkaloids, especially Piperine. However, Piperine synthesis pathway mechanisms are not yet well known. This work was aimed to generate the full-length comparative berry transcriptome analysis dataset of P. nigrum and P. longum by Illumina and Nanopore sequencing platforms. While short-read sequencing technology is widely using to capture transcriptome profiles, there are still some limitations due to the read length. We used Oxford Nanopore technology for long reads and the Illumina sequencing platform for short reads to generate a hybrid transcriptome assembly from half matured and fully matured berries of P. nigrum and P. longum. From P. nigrum and P. longum 37.3 million and 38.1 million raw reads were generated respectively. A total of 308369 contigs from P. nigrum and 267715 contigs from P. longum were obtained and successfully annotated. The transcriptome data revealed gene families involved in piperine and other secondary metabolite biosynthetic pathways. The raw data were uploaded to NCBI database. This dataset shed light on the further exploration of the piperine biosynthetic pathway, its transcriptomic changes, and evolution. Data generated has been submitted to SRA of NCBI with Bio samples accession: (SAMN13981803, SAMN22826456).http://www.sciencedirect.com/science/article/pii/S2352340922004632Hybrid transcriptomeIlluminaNanoporePiper nigrumPiper longumPiperine |
| spellingShingle | Johnson K. George M.A. Fayad S. Shelvy T.E. Sheeja Santhosh J. Eapen Sona Charles Anil Rai Dinesh Kumar Dataset of De Novo hybrid berry transcriptome profiling and characterization of Piper species (Piper nigrum and Piper longum) using Illumina and Nanopore sequencing Data in Brief Hybrid transcriptome Illumina Nanopore Piper nigrum Piper longum Piperine |
| title | Dataset of De Novo hybrid berry transcriptome profiling and characterization of Piper species (Piper nigrum and Piper longum) using Illumina and Nanopore sequencing |
| title_full | Dataset of De Novo hybrid berry transcriptome profiling and characterization of Piper species (Piper nigrum and Piper longum) using Illumina and Nanopore sequencing |
| title_fullStr | Dataset of De Novo hybrid berry transcriptome profiling and characterization of Piper species (Piper nigrum and Piper longum) using Illumina and Nanopore sequencing |
| title_full_unstemmed | Dataset of De Novo hybrid berry transcriptome profiling and characterization of Piper species (Piper nigrum and Piper longum) using Illumina and Nanopore sequencing |
| title_short | Dataset of De Novo hybrid berry transcriptome profiling and characterization of Piper species (Piper nigrum and Piper longum) using Illumina and Nanopore sequencing |
| title_sort | dataset of de novo hybrid berry transcriptome profiling and characterization of piper species piper nigrum and piper longum using illumina and nanopore sequencing |
| topic | Hybrid transcriptome Illumina Nanopore Piper nigrum Piper longum Piperine |
| url | http://www.sciencedirect.com/science/article/pii/S2352340922004632 |
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