Molecular Characterization of <i>Fusarium oxysporum</i> G30 Isolated from Vegetable Wastes in Imphal, Northeast India
A new strain of Fusarium oxysporum (Schl.) emend. Synder & Hansen, viz. Fusarium oxysporum G30, is reported from seven different decomposing vegetable wastes and a mixture of the same in equal proportions, in Imphal, Northeast India. This strain was isolated by surface sterilization technique, f...
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| Format: | Article |
| Language: | English |
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Society of Land Measurements and Cadastre from Transylvania (SMTCT)
2015-06-01
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| Series: | Notulae Scientia Biologicae |
| Online Access: | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9514 |
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| author | Gehamanjuri Laimayum SHARMA Pinokiyo ATHOKPAM Bijaya Devi THONGATABAM |
| author_facet | Gehamanjuri Laimayum SHARMA Pinokiyo ATHOKPAM Bijaya Devi THONGATABAM |
| author_sort | Gehamanjuri Laimayum SHARMA |
| collection | DOAJ |
| description | A new strain of Fusarium oxysporum (Schl.) emend. Synder & Hansen, viz. Fusarium oxysporum G30, is reported from seven different decomposing vegetable wastes and a mixture of the same in equal proportions, in Imphal, Northeast India. This strain was isolated by surface sterilization technique, followed by re-culturing till pure culture was obtained. The pure culture was characterised morphologically based on the colony features: colour, texture, colony diameter; conidial features: macro and microconidial shape, size, septation, presence of chlamydospores and foot-shaped basal cells. The strain was also identified at molecular level by partial sequencing using universal primers, ITS4 and ITS5. The ITS data reveals that isolated culture (F. oxysporum G30) match the existing isolates in Gen Bank (Gen Bank accession No. GQ497156.1) by 99% and it has got a code segment particular to this strain. This strain showed ubiquitous nature with mean percent frequency of occurrence ranging from 39.5 to 68% at various stages of decomposition of the selected vegetable wastes. |
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| institution | Directory Open Access Journal |
| issn | 2067-3205 2067-3264 |
| language | English |
| last_indexed | 2024-04-13T19:51:46Z |
| publishDate | 2015-06-01 |
| publisher | Society of Land Measurements and Cadastre from Transylvania (SMTCT) |
| record_format | Article |
| series | Notulae Scientia Biologicae |
| spelling | doaj.art-4bc2889a205d4e4a8d05105ab767c1312022-12-22T02:32:30ZengSociety of Land Measurements and Cadastre from Transylvania (SMTCT)Notulae Scientia Biologicae2067-32052067-32642015-06-017218418710.15835/nsb7295148136Molecular Characterization of <i>Fusarium oxysporum</i> G30 Isolated from Vegetable Wastes in Imphal, Northeast IndiaGehamanjuri Laimayum SHARMA0Pinokiyo ATHOKPAM1Bijaya Devi THONGATABAM2Manipur University, Department of Life Sciences, Canchipur, Imphal-03Dhanamanjuri College of Science, Department of Botany, Imphal-01University of Mysore, Department of Studies in Zoology, KarnatakaA new strain of Fusarium oxysporum (Schl.) emend. Synder & Hansen, viz. Fusarium oxysporum G30, is reported from seven different decomposing vegetable wastes and a mixture of the same in equal proportions, in Imphal, Northeast India. This strain was isolated by surface sterilization technique, followed by re-culturing till pure culture was obtained. The pure culture was characterised morphologically based on the colony features: colour, texture, colony diameter; conidial features: macro and microconidial shape, size, septation, presence of chlamydospores and foot-shaped basal cells. The strain was also identified at molecular level by partial sequencing using universal primers, ITS4 and ITS5. The ITS data reveals that isolated culture (F. oxysporum G30) match the existing isolates in Gen Bank (Gen Bank accession No. GQ497156.1) by 99% and it has got a code segment particular to this strain. This strain showed ubiquitous nature with mean percent frequency of occurrence ranging from 39.5 to 68% at various stages of decomposition of the selected vegetable wastes.http://www.notulaebiologicae.ro/index.php/nsb/article/view/9514 |
| spellingShingle | Gehamanjuri Laimayum SHARMA Pinokiyo ATHOKPAM Bijaya Devi THONGATABAM Molecular Characterization of <i>Fusarium oxysporum</i> G30 Isolated from Vegetable Wastes in Imphal, Northeast India Notulae Scientia Biologicae |
| title | Molecular Characterization of <i>Fusarium oxysporum</i> G30 Isolated from Vegetable Wastes in Imphal, Northeast India |
| title_full | Molecular Characterization of <i>Fusarium oxysporum</i> G30 Isolated from Vegetable Wastes in Imphal, Northeast India |
| title_fullStr | Molecular Characterization of <i>Fusarium oxysporum</i> G30 Isolated from Vegetable Wastes in Imphal, Northeast India |
| title_full_unstemmed | Molecular Characterization of <i>Fusarium oxysporum</i> G30 Isolated from Vegetable Wastes in Imphal, Northeast India |
| title_short | Molecular Characterization of <i>Fusarium oxysporum</i> G30 Isolated from Vegetable Wastes in Imphal, Northeast India |
| title_sort | molecular characterization of lt i gt fusarium oxysporum lt i gt g30 isolated from vegetable wastes in imphal northeast india |
| url | http://www.notulaebiologicae.ro/index.php/nsb/article/view/9514 |
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