Dietary Supplementation with 20-Hydroxyecdysone Ameliorates Hepatic Steatosis and Reduces White Adipose Tissue Mass in Ovariectomized Rats Fed a High-Fat, High-Fructose Diet
Metabolic dysfunction-associated fatty liver disease (MAFLD) is defined as hepatic steatosis in combination with overweight, diabetes, or other metabolic risk factors. MAFLD affects a significant number of the global population and imposes substantial clinical and economic burdens. With no approved...
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MDPI AG
2023-07-01
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| Series: | Biomedicines |
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| Online Access: | https://www.mdpi.com/2227-9059/11/7/2071 |
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| author | Jariya Buniam Piyachat Chansela Jittima Weerachayaphorn Vitoon Saengsirisuwan |
| author_facet | Jariya Buniam Piyachat Chansela Jittima Weerachayaphorn Vitoon Saengsirisuwan |
| author_sort | Jariya Buniam |
| collection | DOAJ |
| description | Metabolic dysfunction-associated fatty liver disease (MAFLD) is defined as hepatic steatosis in combination with overweight, diabetes, or other metabolic risk factors. MAFLD affects a significant number of the global population and imposes substantial clinical and economic burdens. With no approved pharmacotherapy, current treatment options are limited to diet and exercise. Therefore, the development of medicines for MAFLD treatment or prevention is necessary. 20-Hydroxyecdysone (20E) is a natural steroid found in edible plants and has been shown to improve metabolism and dyslipidemia. Therefore, it may be useful for MAFLD treatment. Here, we aimed to determine how dietary supplementation with 20E affects fat accumulation and lipogenesis in the liver and adipose tissue of ovariectomized rats fed a high-fat, high-fructose diet (OHFFD). We found that 20E reduced hepatic triglyceride content and visceral fat deposition. 20E increased the phosphorylation of AMP-activated protein kinase and acetyl CoA carboxylase while reducing the expression of fatty acid synthase in the liver and adipose tissue. Additionally, 20E increased hepatic expression of carnitine palmitoyltransferase-1 and reduced adipose expression of sterol regulatory element-binding protein-1. In conclusion, 20E demonstrated beneficial effects in rats with OHFFD-induced MAFLD. These findings suggest that 20E may represent a promising option for MAFLD prevention or treatment. |
| first_indexed | 2024-03-11T01:15:26Z |
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| id | doaj.art-4bc56ec6828843fabb50699cb4634e70 |
| institution | Directory Open Access Journal |
| issn | 2227-9059 |
| language | English |
| last_indexed | 2024-03-11T01:15:26Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomedicines |
| spelling | doaj.art-4bc56ec6828843fabb50699cb4634e702023-11-18T18:29:04ZengMDPI AGBiomedicines2227-90592023-07-01117207110.3390/biomedicines11072071Dietary Supplementation with 20-Hydroxyecdysone Ameliorates Hepatic Steatosis and Reduces White Adipose Tissue Mass in Ovariectomized Rats Fed a High-Fat, High-Fructose DietJariya Buniam0Piyachat Chansela1Jittima Weerachayaphorn2Vitoon Saengsirisuwan3Princess Srisavangavadhana College of Medicine, Chulabhorn Royal Academy, Bangkok 10210, ThailandDepartment of Anatomy, Phramongkutklao College of Medicine, Bangkok 10400, ThailandDepartment of Physiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandDepartment of Physiology, Faculty of Science, Mahidol University, Bangkok 10400, ThailandMetabolic dysfunction-associated fatty liver disease (MAFLD) is defined as hepatic steatosis in combination with overweight, diabetes, or other metabolic risk factors. MAFLD affects a significant number of the global population and imposes substantial clinical and economic burdens. With no approved pharmacotherapy, current treatment options are limited to diet and exercise. Therefore, the development of medicines for MAFLD treatment or prevention is necessary. 20-Hydroxyecdysone (20E) is a natural steroid found in edible plants and has been shown to improve metabolism and dyslipidemia. Therefore, it may be useful for MAFLD treatment. Here, we aimed to determine how dietary supplementation with 20E affects fat accumulation and lipogenesis in the liver and adipose tissue of ovariectomized rats fed a high-fat, high-fructose diet (OHFFD). We found that 20E reduced hepatic triglyceride content and visceral fat deposition. 20E increased the phosphorylation of AMP-activated protein kinase and acetyl CoA carboxylase while reducing the expression of fatty acid synthase in the liver and adipose tissue. Additionally, 20E increased hepatic expression of carnitine palmitoyltransferase-1 and reduced adipose expression of sterol regulatory element-binding protein-1. In conclusion, 20E demonstrated beneficial effects in rats with OHFFD-induced MAFLD. These findings suggest that 20E may represent a promising option for MAFLD prevention or treatment.https://www.mdpi.com/2227-9059/11/7/207120-hydroxyecdysonemetabolic dysfunction-associated fatty liver disease (MAFLD)ovariectomyhigh-fat high-fructose dietobesity |
| spellingShingle | Jariya Buniam Piyachat Chansela Jittima Weerachayaphorn Vitoon Saengsirisuwan Dietary Supplementation with 20-Hydroxyecdysone Ameliorates Hepatic Steatosis and Reduces White Adipose Tissue Mass in Ovariectomized Rats Fed a High-Fat, High-Fructose Diet Biomedicines 20-hydroxyecdysone metabolic dysfunction-associated fatty liver disease (MAFLD) ovariectomy high-fat high-fructose diet obesity |
| title | Dietary Supplementation with 20-Hydroxyecdysone Ameliorates Hepatic Steatosis and Reduces White Adipose Tissue Mass in Ovariectomized Rats Fed a High-Fat, High-Fructose Diet |
| title_full | Dietary Supplementation with 20-Hydroxyecdysone Ameliorates Hepatic Steatosis and Reduces White Adipose Tissue Mass in Ovariectomized Rats Fed a High-Fat, High-Fructose Diet |
| title_fullStr | Dietary Supplementation with 20-Hydroxyecdysone Ameliorates Hepatic Steatosis and Reduces White Adipose Tissue Mass in Ovariectomized Rats Fed a High-Fat, High-Fructose Diet |
| title_full_unstemmed | Dietary Supplementation with 20-Hydroxyecdysone Ameliorates Hepatic Steatosis and Reduces White Adipose Tissue Mass in Ovariectomized Rats Fed a High-Fat, High-Fructose Diet |
| title_short | Dietary Supplementation with 20-Hydroxyecdysone Ameliorates Hepatic Steatosis and Reduces White Adipose Tissue Mass in Ovariectomized Rats Fed a High-Fat, High-Fructose Diet |
| title_sort | dietary supplementation with 20 hydroxyecdysone ameliorates hepatic steatosis and reduces white adipose tissue mass in ovariectomized rats fed a high fat high fructose diet |
| topic | 20-hydroxyecdysone metabolic dysfunction-associated fatty liver disease (MAFLD) ovariectomy high-fat high-fructose diet obesity |
| url | https://www.mdpi.com/2227-9059/11/7/2071 |
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