Effect of a Synbiotic Supplement on Fear Response and Memory Assessment of Broiler Chickens Subjected to Heat Stress
The aim of this study was to evaluate the effect of a synbiotic containing a probiotic (<i>Enterococcus faecium</i>, <i>Pediococcus acidilactici</i>, <i>Bifidobacterium animalis</i>, and <i>Lactobacillus reuteri</i>) and a prebiotic (fructooligosacchar...
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MDPI AG
2021-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/11/2/427 |
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| author | Ahmed Mohammed Manal Mahmoud Raj Murugesan Heng-wei Cheng |
| author_facet | Ahmed Mohammed Manal Mahmoud Raj Murugesan Heng-wei Cheng |
| author_sort | Ahmed Mohammed |
| collection | DOAJ |
| description | The aim of this study was to evaluate the effect of a synbiotic containing a probiotic (<i>Enterococcus faecium</i>, <i>Pediococcus acidilactici</i>, <i>Bifidobacterium animalis</i>, and <i>Lactobacillus reuteri</i>) and a prebiotic (fructooligosaccharides) on fear response, memory assessment, and selected stress indicators in broilers subjected to heat stress. A total of 360 1-day-old Ross 708 chicks were evenly divided among three treatments: a basal diet mixed with a synbiotic at 0 (G-C), 0.5 (G-0.5X), and 1.0 (G-1.0X) g/kg. After 15 d, the broilers were exposed to 32 °C for 9 h daily until 42 d. The object memory test was conducted at 15 day; touch, novel object, and isolation tests were conducted at 35 day; tonic immobility (TI) took place at 41 day. At 42 day, plasma corticosterone and tryptophan concentrations and heterophile/lymphocyte (H/L) ratios were measured. Compared to controls, synbiotic-fed broilers, regardless of concentration, had a shorter latency to make the first vocalization, with higher vocalization rates during the isolation test (<i>p</i> = 0.001). the G-1.0 group had the lowest H/L ratio (<i>p</i> = 0.001), but higher plasma tryptophan concentrations and a greater number of birds could reach the observer during the touch test (<i>p</i> = 0.001 and 0.043, respectively). The current results indicate that the synbiotic can be used as a growth promoter to reduce the fear response and stress state of heat-stressed broilers. |
| first_indexed | 2024-03-09T05:18:02Z |
| format | Article |
| id | doaj.art-4bca10523dd547e68b5c97aec325dc0a |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-09T05:18:02Z |
| publishDate | 2021-02-01 |
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| series | Animals |
| spelling | doaj.art-4bca10523dd547e68b5c97aec325dc0a2023-12-03T12:43:46ZengMDPI AGAnimals2076-26152021-02-0111242710.3390/ani11020427Effect of a Synbiotic Supplement on Fear Response and Memory Assessment of Broiler Chickens Subjected to Heat StressAhmed Mohammed0Manal Mahmoud1Raj Murugesan2Heng-wei Cheng3Department of Animal Sciences, Purdue University, 915 West State Street, West Lafayette, IN 47907, USADepartment of Animal Hygiene, Faculty of Veterinary Medicine, Assiut University, Assiut 71526, EgyptBIOMIN America Inc., Overland Park, KS 66210, USAUSDA Agricultural Research Service, 125 South Russell Street, West Lafayette, IN 47907, USAThe aim of this study was to evaluate the effect of a synbiotic containing a probiotic (<i>Enterococcus faecium</i>, <i>Pediococcus acidilactici</i>, <i>Bifidobacterium animalis</i>, and <i>Lactobacillus reuteri</i>) and a prebiotic (fructooligosaccharides) on fear response, memory assessment, and selected stress indicators in broilers subjected to heat stress. A total of 360 1-day-old Ross 708 chicks were evenly divided among three treatments: a basal diet mixed with a synbiotic at 0 (G-C), 0.5 (G-0.5X), and 1.0 (G-1.0X) g/kg. After 15 d, the broilers were exposed to 32 °C for 9 h daily until 42 d. The object memory test was conducted at 15 day; touch, novel object, and isolation tests were conducted at 35 day; tonic immobility (TI) took place at 41 day. At 42 day, plasma corticosterone and tryptophan concentrations and heterophile/lymphocyte (H/L) ratios were measured. Compared to controls, synbiotic-fed broilers, regardless of concentration, had a shorter latency to make the first vocalization, with higher vocalization rates during the isolation test (<i>p</i> = 0.001). the G-1.0 group had the lowest H/L ratio (<i>p</i> = 0.001), but higher plasma tryptophan concentrations and a greater number of birds could reach the observer during the touch test (<i>p</i> = 0.001 and 0.043, respectively). The current results indicate that the synbiotic can be used as a growth promoter to reduce the fear response and stress state of heat-stressed broilers.https://www.mdpi.com/2076-2615/11/2/427broilersheat stressgut microbiotamental statusgut-brain axisstress indicator |
| spellingShingle | Ahmed Mohammed Manal Mahmoud Raj Murugesan Heng-wei Cheng Effect of a Synbiotic Supplement on Fear Response and Memory Assessment of Broiler Chickens Subjected to Heat Stress Animals broilers heat stress gut microbiota mental status gut-brain axis stress indicator |
| title | Effect of a Synbiotic Supplement on Fear Response and Memory Assessment of Broiler Chickens Subjected to Heat Stress |
| title_full | Effect of a Synbiotic Supplement on Fear Response and Memory Assessment of Broiler Chickens Subjected to Heat Stress |
| title_fullStr | Effect of a Synbiotic Supplement on Fear Response and Memory Assessment of Broiler Chickens Subjected to Heat Stress |
| title_full_unstemmed | Effect of a Synbiotic Supplement on Fear Response and Memory Assessment of Broiler Chickens Subjected to Heat Stress |
| title_short | Effect of a Synbiotic Supplement on Fear Response and Memory Assessment of Broiler Chickens Subjected to Heat Stress |
| title_sort | effect of a synbiotic supplement on fear response and memory assessment of broiler chickens subjected to heat stress |
| topic | broilers heat stress gut microbiota mental status gut-brain axis stress indicator |
| url | https://www.mdpi.com/2076-2615/11/2/427 |
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