Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres.
The uncapping of telomeres induces a DNA damage response. In Schizosaccharomyces pombe, deletion of pot1+ causes telomere uncapping and rapid telomere resection, resulting in chromosome fusion. Using the nmt-pot1-aid strain, we previously reported that Pot1 shut-off causes telomere loss and chromoso...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4605587?pdf=render |
| _version_ | 1819048717120962560 |
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| author | Tomoko Nanbu Luân C Nguyễn Ahmed G K Habib Naoya Hirata Shinobu Ukimori Daiki Tanaka Kenta Masuda Katsunori Takahashi Masashi Yukawa Eiko Tsuchiya Masaru Ueno |
| author_facet | Tomoko Nanbu Luân C Nguyễn Ahmed G K Habib Naoya Hirata Shinobu Ukimori Daiki Tanaka Kenta Masuda Katsunori Takahashi Masashi Yukawa Eiko Tsuchiya Masaru Ueno |
| author_sort | Tomoko Nanbu |
| collection | DOAJ |
| description | The uncapping of telomeres induces a DNA damage response. In Schizosaccharomyces pombe, deletion of pot1+ causes telomere uncapping and rapid telomere resection, resulting in chromosome fusion. Using the nmt-pot1-aid strain, we previously reported that Pot1 shut-off causes telomere loss and chromosome fusion in S. pombe. However, the factors responsible for the resection of uncapped telomeres remain unknown. In this study, we investigated these factors and found that concomitant deletion of rqh1+ and exo1+ alleviated the loss of telomeres following Pot1 shut-off, suggesting that Rqh1 and Exo1 are redundantly involved in the resection of uncapped telomeres. We also investigated the role of Rqh1 helicase activity and found it to be essential for the resection of uncapped telomeres. Moreover, we found that Dna2 and Exo1 function redundantly in the resection of uncapped telomeres. Taken together, these results suggest that Exo1 and Rqh1-Dna2 redundantly contribute to the resection of uncapped telomeres. Therefore, our results demonstrate that nmt-pot1-aid is an important model strain to study the role of helicases and nucleases in the resection of uncapped telomeres and to improve our understanding of DNA double-strand break repair. |
| first_indexed | 2024-12-21T11:20:41Z |
| format | Article |
| id | doaj.art-4bcc363bd672429c834725231787d1f1 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-21T11:20:41Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4bcc363bd672429c834725231787d1f12022-12-21T19:05:48ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-011010e014045610.1371/journal.pone.0140456Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres.Tomoko NanbuLuân C NguyễnAhmed G K HabibNaoya HirataShinobu UkimoriDaiki TanakaKenta MasudaKatsunori TakahashiMasashi YukawaEiko TsuchiyaMasaru UenoThe uncapping of telomeres induces a DNA damage response. In Schizosaccharomyces pombe, deletion of pot1+ causes telomere uncapping and rapid telomere resection, resulting in chromosome fusion. Using the nmt-pot1-aid strain, we previously reported that Pot1 shut-off causes telomere loss and chromosome fusion in S. pombe. However, the factors responsible for the resection of uncapped telomeres remain unknown. In this study, we investigated these factors and found that concomitant deletion of rqh1+ and exo1+ alleviated the loss of telomeres following Pot1 shut-off, suggesting that Rqh1 and Exo1 are redundantly involved in the resection of uncapped telomeres. We also investigated the role of Rqh1 helicase activity and found it to be essential for the resection of uncapped telomeres. Moreover, we found that Dna2 and Exo1 function redundantly in the resection of uncapped telomeres. Taken together, these results suggest that Exo1 and Rqh1-Dna2 redundantly contribute to the resection of uncapped telomeres. Therefore, our results demonstrate that nmt-pot1-aid is an important model strain to study the role of helicases and nucleases in the resection of uncapped telomeres and to improve our understanding of DNA double-strand break repair.http://europepmc.org/articles/PMC4605587?pdf=render |
| spellingShingle | Tomoko Nanbu Luân C Nguyễn Ahmed G K Habib Naoya Hirata Shinobu Ukimori Daiki Tanaka Kenta Masuda Katsunori Takahashi Masashi Yukawa Eiko Tsuchiya Masaru Ueno Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres. PLoS ONE |
| title | Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres. |
| title_full | Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres. |
| title_fullStr | Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres. |
| title_full_unstemmed | Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres. |
| title_short | Fission Yeast Exo1 and Rqh1-Dna2 Redundantly Contribute to Resection of Uncapped Telomeres. |
| title_sort | fission yeast exo1 and rqh1 dna2 redundantly contribute to resection of uncapped telomeres |
| url | http://europepmc.org/articles/PMC4605587?pdf=render |
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