Bacteriocinogenic Lactic Acid Bacteria Isolated from Mangrove Sediment in Indonesia: Growth Optimization, Bacteriocin Production, and its Application in Food Preservation
The mangrove ecosystem is unique because it is located between marine and land. Little research is exploring lactic acid bacteria (LAB) from mangrove ecosystems. The LAB LG71 isolate is successfully isolated from the sediment of mangrove ecosystems on the coast of Logending, Jawa Tengah (Indonesia)...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2023-08-01
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| Series: | Hayati Journal of Biosciences |
| Online Access: | https://journal.ipb.ac.id/index.php/hayati/article/view/46146 |
| _version_ | 1797689919603212288 |
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| author | Dyah Fitri Kusharyati Oedjijono Taruna Dwi Satwika Dwiana Muflihah Yulianti Afifah Mariana Anwar Rovik |
| author_facet | Dyah Fitri Kusharyati Oedjijono Taruna Dwi Satwika Dwiana Muflihah Yulianti Afifah Mariana Anwar Rovik |
| author_sort | Dyah Fitri Kusharyati |
| collection | DOAJ |
| description |
The mangrove ecosystem is unique because it is located between marine and land. Little research is exploring lactic acid bacteria (LAB) from mangrove ecosystems. The LAB LG71 isolate is successfully isolated from the sediment of mangrove ecosystems on the coast of Logending, Jawa Tengah (Indonesia). In this study, we aimed to know the effect of glucose supplementation on the growth of LG71 isolate, bacteriocin production, and its application in food preservation. The characterization results showed that the LG71 isolate is Lactobacillus sp. Interestingly, the LG71 isolate is catalase-positive since this character is rarely found in the LAB group. Supplementing 0.25% glucose to MRSB medium and an incubation time of 15 hours is the best treatment for producing Lactobacillus LG71 isolate cell biomass. A 2% concentration of crude extract of Lactobacillus LG71 bacteriocins is the best concentration against Salmonella typhi both during in vitro and in vivo tests in fish balls. The addition of glucose affects the production of Lactobacillus LG71 cell biomass, and the bacteriocin derived from Lactobacillus LG71 gives increased protection against S. typhi and offers an alternative for food preservation.
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| first_indexed | 2024-03-12T01:53:10Z |
| format | Article |
| id | doaj.art-4bcd53f2f4d04f489a8b6d0b8c294629 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-03-12T01:53:10Z |
| publishDate | 2023-08-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-4bcd53f2f4d04f489a8b6d0b8c2946292023-09-08T08:39:37ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942023-08-0130610.4308/hjb.30.6.1121-1131Bacteriocinogenic Lactic Acid Bacteria Isolated from Mangrove Sediment in Indonesia: Growth Optimization, Bacteriocin Production, and its Application in Food PreservationDyah Fitri Kusharyati0Oedjijono1Taruna Dwi Satwika2Dwiana Muflihah Yulianti3Afifah Mariana4Anwar Rovik5Department of Microbiology, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto, Banyumas 53122, IndonesiaDepartment of Microbiology, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto, Banyumas 53122, IndonesiaDepartment of Microbiology, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto, Banyumas 53122, IndonesiaDepartment of Microbiology, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto, Banyumas 53122, IndonesiaDepartment of Microbiology, Faculty of Biology, Universitas Jenderal Soedirman, Purwokerto, Banyumas 53122, IndonesiaCenter for Tropical Medicine, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada, Sleman, Yogyakarta 53288, Indonesia The mangrove ecosystem is unique because it is located between marine and land. Little research is exploring lactic acid bacteria (LAB) from mangrove ecosystems. The LAB LG71 isolate is successfully isolated from the sediment of mangrove ecosystems on the coast of Logending, Jawa Tengah (Indonesia). In this study, we aimed to know the effect of glucose supplementation on the growth of LG71 isolate, bacteriocin production, and its application in food preservation. The characterization results showed that the LG71 isolate is Lactobacillus sp. Interestingly, the LG71 isolate is catalase-positive since this character is rarely found in the LAB group. Supplementing 0.25% glucose to MRSB medium and an incubation time of 15 hours is the best treatment for producing Lactobacillus LG71 isolate cell biomass. A 2% concentration of crude extract of Lactobacillus LG71 bacteriocins is the best concentration against Salmonella typhi both during in vitro and in vivo tests in fish balls. The addition of glucose affects the production of Lactobacillus LG71 cell biomass, and the bacteriocin derived from Lactobacillus LG71 gives increased protection against S. typhi and offers an alternative for food preservation. https://journal.ipb.ac.id/index.php/hayati/article/view/46146 |
| spellingShingle | Dyah Fitri Kusharyati Oedjijono Taruna Dwi Satwika Dwiana Muflihah Yulianti Afifah Mariana Anwar Rovik Bacteriocinogenic Lactic Acid Bacteria Isolated from Mangrove Sediment in Indonesia: Growth Optimization, Bacteriocin Production, and its Application in Food Preservation Hayati Journal of Biosciences |
| title | Bacteriocinogenic Lactic Acid Bacteria Isolated from Mangrove Sediment in Indonesia: Growth Optimization, Bacteriocin Production, and its Application in Food Preservation |
| title_full | Bacteriocinogenic Lactic Acid Bacteria Isolated from Mangrove Sediment in Indonesia: Growth Optimization, Bacteriocin Production, and its Application in Food Preservation |
| title_fullStr | Bacteriocinogenic Lactic Acid Bacteria Isolated from Mangrove Sediment in Indonesia: Growth Optimization, Bacteriocin Production, and its Application in Food Preservation |
| title_full_unstemmed | Bacteriocinogenic Lactic Acid Bacteria Isolated from Mangrove Sediment in Indonesia: Growth Optimization, Bacteriocin Production, and its Application in Food Preservation |
| title_short | Bacteriocinogenic Lactic Acid Bacteria Isolated from Mangrove Sediment in Indonesia: Growth Optimization, Bacteriocin Production, and its Application in Food Preservation |
| title_sort | bacteriocinogenic lactic acid bacteria isolated from mangrove sediment in indonesia growth optimization bacteriocin production and its application in food preservation |
| url | https://journal.ipb.ac.id/index.php/hayati/article/view/46146 |
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