Expression of extracellular matrix components in the meibomian gland
PurposeExtracellular matrix (ECM) is a key component of the stem cell local microenvironment. Our study aims to explore the periglandular distribution of major components of ECM in the Meibomian gland (MG).MethodsHuman eyelids and mouse eyelids were collected and processed for immunofluorescence sta...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Medicine |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmed.2022.981610/full |
| _version_ | 1811210888549498880 |
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| author | Di Chen Xiaomin Chen Hua-Tao Xie Mark P. Hatton Xiaowei Liu Yang Liu |
| author_facet | Di Chen Xiaomin Chen Hua-Tao Xie Mark P. Hatton Xiaowei Liu Yang Liu |
| author_sort | Di Chen |
| collection | DOAJ |
| description | PurposeExtracellular matrix (ECM) is a key component of the stem cell local microenvironment. Our study aims to explore the periglandular distribution of major components of ECM in the Meibomian gland (MG).MethodsHuman eyelids and mouse eyelids were collected and processed for immunofluorescence staining.ResultsHuman MG tissues stained positive for collagen IV α1, collagen IV α2, collagen IV α5, and collagen IV α6 around the acini and duct, but negative for collagen IV α3 and collagen IV α4. The mouse MG were stained positive for the same collagen IV subunits as early as postnatal day 15. Laminin α2, laminin β1 and perlecan stained the regions surrounding the acini and the acinar/ductal junction in the human MG, but not the region around the duct. Tenascin-C was found specifically located at the junctions between the acini and the central ducts. Neither agrin nor endostatin was found in the human MG tissues.ConclusionThe ECM expresses specific components in different regions around the MG, which may play a role in MG stem cell regulation, renewal, and regeneration. |
| first_indexed | 2024-04-12T05:03:25Z |
| format | Article |
| id | doaj.art-4bd1484a0b7b48308abe657c14c6b48c |
| institution | Directory Open Access Journal |
| issn | 2296-858X |
| language | English |
| last_indexed | 2024-04-12T05:03:25Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Medicine |
| spelling | doaj.art-4bd1484a0b7b48308abe657c14c6b48c2022-12-22T03:46:56ZengFrontiers Media S.A.Frontiers in Medicine2296-858X2022-09-01910.3389/fmed.2022.981610981610Expression of extracellular matrix components in the meibomian glandDi Chen0Xiaomin Chen1Hua-Tao Xie2Mark P. Hatton3Xiaowei Liu4Yang Liu5Department of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaDepartment of Ophthalmology, Zhongnan Hospital, Wuhan University, Wuhan, ChinaDepartment of Ophthalmology, Union Hospital, Huazhong University of Science and Technology, Wuhan, ChinaOphthalmic Consultants of Boston, Boston, MA, United StatesDepartment of Ophthalmology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, ChinaDepartment of Ophthalmology, Zhongnan Hospital, Wuhan University, Wuhan, ChinaPurposeExtracellular matrix (ECM) is a key component of the stem cell local microenvironment. Our study aims to explore the periglandular distribution of major components of ECM in the Meibomian gland (MG).MethodsHuman eyelids and mouse eyelids were collected and processed for immunofluorescence staining.ResultsHuman MG tissues stained positive for collagen IV α1, collagen IV α2, collagen IV α5, and collagen IV α6 around the acini and duct, but negative for collagen IV α3 and collagen IV α4. The mouse MG were stained positive for the same collagen IV subunits as early as postnatal day 15. Laminin α2, laminin β1 and perlecan stained the regions surrounding the acini and the acinar/ductal junction in the human MG, but not the region around the duct. Tenascin-C was found specifically located at the junctions between the acini and the central ducts. Neither agrin nor endostatin was found in the human MG tissues.ConclusionThe ECM expresses specific components in different regions around the MG, which may play a role in MG stem cell regulation, renewal, and regeneration.https://www.frontiersin.org/articles/10.3389/fmed.2022.981610/fullmeibomian glandextracellar matrixstem cell nichebiomarkerorgan regeneration |
| spellingShingle | Di Chen Xiaomin Chen Hua-Tao Xie Mark P. Hatton Xiaowei Liu Yang Liu Expression of extracellular matrix components in the meibomian gland Frontiers in Medicine meibomian gland extracellar matrix stem cell niche biomarker organ regeneration |
| title | Expression of extracellular matrix components in the meibomian gland |
| title_full | Expression of extracellular matrix components in the meibomian gland |
| title_fullStr | Expression of extracellular matrix components in the meibomian gland |
| title_full_unstemmed | Expression of extracellular matrix components in the meibomian gland |
| title_short | Expression of extracellular matrix components in the meibomian gland |
| title_sort | expression of extracellular matrix components in the meibomian gland |
| topic | meibomian gland extracellar matrix stem cell niche biomarker organ regeneration |
| url | https://www.frontiersin.org/articles/10.3389/fmed.2022.981610/full |
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