Identification of termites from Gabon using MALDI-TOF MS
Termites are one of the most common pests that damage wood and other cellulosic materials. Although Africa has more varieties of termite species than any other continent, few entomological studies have been conducted in Gabon. Identifying termites poses significant difficulties for entomologists. Th...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2024-03-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024041124 |
| _version_ | 1827293918497079296 |
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| author | Bouthaina Hasnaoui Adama Zan Diarra Patrice Makouloutou-Nzassi Jean-Michel Bérenger Afaf Hamame Barthelemy Ngoubangoye Mapenda Gaye Bernard Davoust Oleg Mediannikov Jean Bernard Lekana-Douki Philippe Parola |
| author_facet | Bouthaina Hasnaoui Adama Zan Diarra Patrice Makouloutou-Nzassi Jean-Michel Bérenger Afaf Hamame Barthelemy Ngoubangoye Mapenda Gaye Bernard Davoust Oleg Mediannikov Jean Bernard Lekana-Douki Philippe Parola |
| author_sort | Bouthaina Hasnaoui |
| collection | DOAJ |
| description | Termites are one of the most common pests that damage wood and other cellulosic materials. Although Africa has more varieties of termite species than any other continent, few entomological studies have been conducted in Gabon. Identifying termites poses significant difficulties for entomologists. The aim of this study was to evaluate the reliability and confirm the significance of MALDI-TOF MS in identifying fresh termites collected in equatorial Africa.A total of 108 termites were collected from 13 termite nests during a field mission in 2021 in Lekedi and Bongoville, Gabon. Termites were morphologically identified and subjected to MALDI-TOF MS, then molecular analyses using the COI and 12S rRNA genes.Four termite species were morphologically identified in this study: Pseudacanthotermes militaris, Macrotermes muelleri, Macrotermes nobilis, and Noditermes indoensis. However, when using molecular biology, only three species were identified, namely Macrotermes bellicosus, P. militaris, and N. indoensis, because the specimens initially identified as M. muelleri and M. nobilis were found to be M. bellicosus.The MALDI-TOF MS spectral profiles of the termites were all of good quality, with intra-species reproducibility and inter-species specificity. The spectra of 98 termites were blind tested against our upgraded database, which included the spectra of ten termite specimens. All tested spectra were correctly matched to their respective species, with log score values (LSVs) ranging from 1.649 to 2.592. The mean LSV was 2.215 ± 0.203, and the median was 2.241. However, 95.91% (94/98) of our spectra had LSVs above 1.8. This study demonstrates how a proteomic approach can overcome termites' molecular and morphological identification limitations and serve as a useful taxonomic tool. |
| first_indexed | 2024-04-24T13:48:07Z |
| format | Article |
| id | doaj.art-4bd2759717064210b9e68822a6a6fa04 |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-04-24T13:48:07Z |
| publishDate | 2024-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-4bd2759717064210b9e68822a6a6fa042024-04-04T05:06:47ZengElsevierHeliyon2405-84402024-03-01106e28081Identification of termites from Gabon using MALDI-TOF MSBouthaina Hasnaoui0Adama Zan Diarra1Patrice Makouloutou-Nzassi2Jean-Michel Bérenger3Afaf Hamame4Barthelemy Ngoubangoye5Mapenda Gaye6Bernard Davoust7Oleg Mediannikov8Jean Bernard Lekana-Douki9Philippe Parola10Aix-Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France; IHU Méditerranée Infection, Marseille, FranceAix-Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France; IHU Méditerranée Infection, Marseille, FranceUnité de Recherches en Ecologie de La Santé (URES), Centre Interdisciplinaire de Recherches Médicales de Franceville, B.P. 769, Franceville, Gabon; Institut de Recherches en Ecologie Tropicale (IRET-CENAREST), B.P. 13354, Libreville, GabonAix-Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France; IHU Méditerranée Infection, Marseille, FranceIHU Méditerranée Infection, Marseille, France; Aix-Marseille Univ, IRD, AP-HM, MEPHI, Marseille, FranceCentre de Primatologie, Centre Interdisciplinaire de Recherches Médicales de Franceville, B.P. 769, Franceville, GabonIHU Méditerranée Infection, Marseille, France; Aix-Marseille Univ, IRD, AP-HM, MEPHI, Marseille, FranceIHU Méditerranée Infection, Marseille, France; Aix-Marseille Univ, IRD, AP-HM, MEPHI, Marseille, FranceIHU Méditerranée Infection, Marseille, France; Aix-Marseille Univ, IRD, AP-HM, MEPHI, Marseille, FranceUnité D’Evolution, Epidémiologie et Résistances Parasitaires, Centre Interdisciplinaire de Recherches Médicales de Franceville, B.P. 769, Franceville, Gabon; Département de Parasitologie- Mycologie, Université des Sciences de La Santé, B.P. 4009, Libreville, GabonAix-Marseille Univ, IRD, SSA, AP-HM, VITROME, Marseille, France; IHU Méditerranée Infection, Marseille, France; Corresponding author. VITROME, Institut Hospitalo-Universitaire Méditerranée Infection, 19–21 Boulevard Jean Moulin, 13005, Marseille, France.Termites are one of the most common pests that damage wood and other cellulosic materials. Although Africa has more varieties of termite species than any other continent, few entomological studies have been conducted in Gabon. Identifying termites poses significant difficulties for entomologists. The aim of this study was to evaluate the reliability and confirm the significance of MALDI-TOF MS in identifying fresh termites collected in equatorial Africa.A total of 108 termites were collected from 13 termite nests during a field mission in 2021 in Lekedi and Bongoville, Gabon. Termites were morphologically identified and subjected to MALDI-TOF MS, then molecular analyses using the COI and 12S rRNA genes.Four termite species were morphologically identified in this study: Pseudacanthotermes militaris, Macrotermes muelleri, Macrotermes nobilis, and Noditermes indoensis. However, when using molecular biology, only three species were identified, namely Macrotermes bellicosus, P. militaris, and N. indoensis, because the specimens initially identified as M. muelleri and M. nobilis were found to be M. bellicosus.The MALDI-TOF MS spectral profiles of the termites were all of good quality, with intra-species reproducibility and inter-species specificity. The spectra of 98 termites were blind tested against our upgraded database, which included the spectra of ten termite specimens. All tested spectra were correctly matched to their respective species, with log score values (LSVs) ranging from 1.649 to 2.592. The mean LSV was 2.215 ± 0.203, and the median was 2.241. However, 95.91% (94/98) of our spectra had LSVs above 1.8. This study demonstrates how a proteomic approach can overcome termites' molecular and morphological identification limitations and serve as a useful taxonomic tool.http://www.sciencedirect.com/science/article/pii/S2405844024041124MALDI-TOF MSTermitesIsopteraGabonPseudacanthotermes militarisMacrotermes bellicosus |
| spellingShingle | Bouthaina Hasnaoui Adama Zan Diarra Patrice Makouloutou-Nzassi Jean-Michel Bérenger Afaf Hamame Barthelemy Ngoubangoye Mapenda Gaye Bernard Davoust Oleg Mediannikov Jean Bernard Lekana-Douki Philippe Parola Identification of termites from Gabon using MALDI-TOF MS Heliyon MALDI-TOF MS Termites Isoptera Gabon Pseudacanthotermes militaris Macrotermes bellicosus |
| title | Identification of termites from Gabon using MALDI-TOF MS |
| title_full | Identification of termites from Gabon using MALDI-TOF MS |
| title_fullStr | Identification of termites from Gabon using MALDI-TOF MS |
| title_full_unstemmed | Identification of termites from Gabon using MALDI-TOF MS |
| title_short | Identification of termites from Gabon using MALDI-TOF MS |
| title_sort | identification of termites from gabon using maldi tof ms |
| topic | MALDI-TOF MS Termites Isoptera Gabon Pseudacanthotermes militaris Macrotermes bellicosus |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024041124 |
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