A flexible and economical barcoding approach for highly multiplexed amplicon sequencing of diverse target genes

A flexible and economical barcoding approach for highly multiplexed amplicon sequencing of diverse target genes

High throughput sequencing of phylogenetic and functional gene amplicons provides tremendous insight into the structure and functional potential of complex microbial communities. Here, we introduce a highly adaptable and economical PCR approach to barcoding and pooling libraries of numerous target g...

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Bibliographic Details
Main Authors: Craig W. Herbold, Claus ePelikan, Orest eKuzyk, Bela eHausmann, Roey eAngel, David eBerry, Alexander eLoy
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-07-01
Series:Frontiers in Microbiology
Subjects:
16S rRNA
amoA
dsrA
nifH
functional gene
dsrB
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00731/full

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