Expression of membrane-bound β-Klotho on peripheral blood CD4+ and CD8+ T lymphocytes of healthy subjects
AbstractImmunosenescence is driven by repeated and continuous immune activation. Sensitive markers are warranted to detect and monitor the process of premature immune ageing. We analysed the β-Klotho expression on T-cell subsets from healthy donors: young adults, n = 12 (20–30 y); middle-aged, n = 6...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2023-12-01
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| Series: | Biotechnology & Biotechnological Equipment |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/13102818.2023.2199887 |
| Summary: | AbstractImmunosenescence is driven by repeated and continuous immune activation. Sensitive markers are warranted to detect and monitor the process of premature immune ageing. We analysed the β-Klotho expression on T-cell subsets from healthy donors: young adults, n = 12 (20–30 y); middle-aged, n = 62 (30–50 y) and elderly, n = 18 (>70 y). The absolute counts (AC) and percentages of total, CD4+, CD8+ and CD8 + CD27-CD57+ T-cells, CD4/CD8 ratio, and the share of β-Klotho + T-cell subsets were analysed by multicolour flow cytometry. AC of T-cell subsets showed non-significant differences among the groups. The percentages of β-Klotho(+) total, CD4+ and CD8+ T-cells in young adults exceeded significantly the expression in the elderly and middle-aged subjects: (mean) 2.13 vs. 0.48; 3.23 vs. 0.48; 6.87 vs. 2.28, and 0.75 vs. 2.21; 0.93 vs. 3.32; 6.62 vs. 6.62, respectively (p < 0.05 all). The β-Klotho expression on total, CD4+ and CD8+ T-cells among middle-aged participants was heterogeneous and significantly higher from the elderly only in CD8 + T-cells (mean): 0.75 vs. 0.47 (p = 0.1); 0.93 vs. 0.72 (p = 1.0) and 3.62 vs 1.75 (p = 0.01), respectively. The proportion of β-Klotho(+) CD8+ cells did not correlate with age, but correlated inversely and significantly with the share of CD27-CD57 + CD8+ T-cells in middle-aged subjects (R = −0.4, p < 0.01). We hypothesise that CD8 + T-cells showed higher sensitivity to the effects of immune-senescence, at least in part, due to the higher expression and dependence on the effects of KLOTHO gene products. We propose that a reduced share of β-Klotho + CD8+ T-cells indicates exhaustion due to immune activation, and may serve as an early marker of premature immunosenescence. |
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| ISSN: | 1310-2818 1314-3530 |