Utility of Modified Ultrafast Papanicolaou Stain in Cytological Diagnosis
Introduction: Need for minimal turnaround time for assessing Fine Needle Aspiration Cytology (FNAC) has encouraged innovations in staining techniques that require lesser staining time with unequivocal cell morphology. The standard protocol for conventional Papanicolaou (PAP) stain requires about...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2017-03-01
|
| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/9532/20882_CE[Ra]_F(Sh)_PF1(PI_VJ)_PFA(NC_AK)_PF2(NE_DK).pdf |
| Summary: | Introduction: Need for minimal turnaround time for assessing
Fine Needle Aspiration Cytology (FNAC) has encouraged
innovations in staining techniques that require lesser staining
time with unequivocal cell morphology. The standard protocol
for conventional Papanicolaou (PAP) stain requires about 40
minutes. To overcome this, Ultrafast Papanicolaou (UFP) stain
was introduced which reduces staining time to 90 seconds and
also enhances the quality. However, reagents required for this
were not easily available hence, Modified Ultrafast Papanicolaou
(MUFP) stain was introduced subsequently.
Aim: To assess the efficacy of MUFP staining by comparing the
quality of MUFP stain with conventional PAP stain.
Materials and Methods: FNAC procedure was performed by
using 10 ml disposable syringe and 22-23 G needle. Total 131
FNAC cases were studied which were lymph node (30), thyroid
(38), breast (22), skin and soft tissue (24), salivary gland (11) and
visceral organs (6). Two smears were prepared and stained by
MUFP and conventional PAP stain. Scores were given on four
parameters: background of smears, overall staining pattern,
cell morphology and nuclear staining. Quality Index (QI) was
calculated from ratio of total score achieved to maximum score
possible. Statistical analysis using chi square test was applied
to each of the four parameters before obtaining the QI in both
stains. Students t-test was applied to evaluate the efficacy of
MUFP in comparison with conventional PAP stain.
Results: The QI of MUFP for thyroid, breast, lymph node, skin
and soft tissue, salivary gland and visceral organs was 0.89,
0.85, 0.89, 0.83, 0.92, and 0.78 respectively. Compared to
conventional PAP stain QI of MUFP smears was better in all
except visceral organ cases and was statistically significant.
MUFP showed clear red blood cell background, transparent
cytoplasm and crisp nuclear features.
Conclusion: MUFP is fast, reliable and can be done with locally
available reagents with unequivocal morphology which is the
need of the hour for a cytopathology set-up. |
|---|---|
| ISSN: | 2249-782X 0973-709X |