Utility of Modified Ultrafast Papanicolaou Stain in Cytological Diagnosis
Introduction: Need for minimal turnaround time for assessing Fine Needle Aspiration Cytology (FNAC) has encouraged innovations in staining techniques that require lesser staining time with unequivocal cell morphology. The standard protocol for conventional Papanicolaou (PAP) stain requires about...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2017-03-01
|
| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/9532/20882_CE[Ra]_F(Sh)_PF1(PI_VJ)_PFA(NC_AK)_PF2(NE_DK).pdf |
| _version_ | 1818265069634453504 |
|---|---|
| author | Prachi Sinkar Surekha Ulhas Arakeri |
| author_facet | Prachi Sinkar Surekha Ulhas Arakeri |
| author_sort | Prachi Sinkar |
| collection | DOAJ |
| description | Introduction: Need for minimal turnaround time for assessing
Fine Needle Aspiration Cytology (FNAC) has encouraged
innovations in staining techniques that require lesser staining
time with unequivocal cell morphology. The standard protocol
for conventional Papanicolaou (PAP) stain requires about 40
minutes. To overcome this, Ultrafast Papanicolaou (UFP) stain
was introduced which reduces staining time to 90 seconds and
also enhances the quality. However, reagents required for this
were not easily available hence, Modified Ultrafast Papanicolaou
(MUFP) stain was introduced subsequently.
Aim: To assess the efficacy of MUFP staining by comparing the
quality of MUFP stain with conventional PAP stain.
Materials and Methods: FNAC procedure was performed by
using 10 ml disposable syringe and 22-23 G needle. Total 131
FNAC cases were studied which were lymph node (30), thyroid
(38), breast (22), skin and soft tissue (24), salivary gland (11) and
visceral organs (6). Two smears were prepared and stained by
MUFP and conventional PAP stain. Scores were given on four
parameters: background of smears, overall staining pattern,
cell morphology and nuclear staining. Quality Index (QI) was
calculated from ratio of total score achieved to maximum score
possible. Statistical analysis using chi square test was applied
to each of the four parameters before obtaining the QI in both
stains. Students t-test was applied to evaluate the efficacy of
MUFP in comparison with conventional PAP stain.
Results: The QI of MUFP for thyroid, breast, lymph node, skin
and soft tissue, salivary gland and visceral organs was 0.89,
0.85, 0.89, 0.83, 0.92, and 0.78 respectively. Compared to
conventional PAP stain QI of MUFP smears was better in all
except visceral organ cases and was statistically significant.
MUFP showed clear red blood cell background, transparent
cytoplasm and crisp nuclear features.
Conclusion: MUFP is fast, reliable and can be done with locally
available reagents with unequivocal morphology which is the
need of the hour for a cytopathology set-up. |
| first_indexed | 2024-12-12T19:44:57Z |
| format | Article |
| id | doaj.art-4bd6c343f1b54d079f83b333342df090 |
| institution | Directory Open Access Journal |
| issn | 2249-782X 0973-709X |
| language | English |
| last_indexed | 2024-12-12T19:44:57Z |
| publishDate | 2017-03-01 |
| publisher | JCDR Research and Publications Private Limited |
| record_format | Article |
| series | Journal of Clinical and Diagnostic Research |
| spelling | doaj.art-4bd6c343f1b54d079f83b333342df0902022-12-22T00:14:07ZengJCDR Research and Publications Private LimitedJournal of Clinical and Diagnostic Research2249-782X0973-709X2017-03-01113EC28EC3110.7860/JCDR/2017/20882.9532Utility of Modified Ultrafast Papanicolaou Stain in Cytological DiagnosisPrachi Sinkar0Surekha Ulhas Arakeri1Postgraduate Student, Department of Pathology, BLDE University’s Shri BM Patil Medical College, Hospital and Research Centre, Vijayapur, Karnataka, India.Professor, Department of Pathology, BLDE University’s Shri BM Patil Medical College, Hospital and Research Centre, Vijayapur, Karnataka, India.Introduction: Need for minimal turnaround time for assessing Fine Needle Aspiration Cytology (FNAC) has encouraged innovations in staining techniques that require lesser staining time with unequivocal cell morphology. The standard protocol for conventional Papanicolaou (PAP) stain requires about 40 minutes. To overcome this, Ultrafast Papanicolaou (UFP) stain was introduced which reduces staining time to 90 seconds and also enhances the quality. However, reagents required for this were not easily available hence, Modified Ultrafast Papanicolaou (MUFP) stain was introduced subsequently. Aim: To assess the efficacy of MUFP staining by comparing the quality of MUFP stain with conventional PAP stain. Materials and Methods: FNAC procedure was performed by using 10 ml disposable syringe and 22-23 G needle. Total 131 FNAC cases were studied which were lymph node (30), thyroid (38), breast (22), skin and soft tissue (24), salivary gland (11) and visceral organs (6). Two smears were prepared and stained by MUFP and conventional PAP stain. Scores were given on four parameters: background of smears, overall staining pattern, cell morphology and nuclear staining. Quality Index (QI) was calculated from ratio of total score achieved to maximum score possible. Statistical analysis using chi square test was applied to each of the four parameters before obtaining the QI in both stains. Students t-test was applied to evaluate the efficacy of MUFP in comparison with conventional PAP stain. Results: The QI of MUFP for thyroid, breast, lymph node, skin and soft tissue, salivary gland and visceral organs was 0.89, 0.85, 0.89, 0.83, 0.92, and 0.78 respectively. Compared to conventional PAP stain QI of MUFP smears was better in all except visceral organ cases and was statistically significant. MUFP showed clear red blood cell background, transparent cytoplasm and crisp nuclear features. Conclusion: MUFP is fast, reliable and can be done with locally available reagents with unequivocal morphology which is the need of the hour for a cytopathology set-up.https://jcdr.net/articles/PDF/9532/20882_CE[Ra]_F(Sh)_PF1(PI_VJ)_PFA(NC_AK)_PF2(NE_DK).pdfconventional papanicolaou stainfine needle aspiration cytologyrapid stain |
| spellingShingle | Prachi Sinkar Surekha Ulhas Arakeri Utility of Modified Ultrafast Papanicolaou Stain in Cytological Diagnosis Journal of Clinical and Diagnostic Research conventional papanicolaou stain fine needle aspiration cytology rapid stain |
| title | Utility of Modified Ultrafast Papanicolaou Stain in Cytological Diagnosis |
| title_full | Utility of Modified Ultrafast Papanicolaou Stain in Cytological Diagnosis |
| title_fullStr | Utility of Modified Ultrafast Papanicolaou Stain in Cytological Diagnosis |
| title_full_unstemmed | Utility of Modified Ultrafast Papanicolaou Stain in Cytological Diagnosis |
| title_short | Utility of Modified Ultrafast Papanicolaou Stain in Cytological Diagnosis |
| title_sort | utility of modified ultrafast papanicolaou stain in cytological diagnosis |
| topic | conventional papanicolaou stain fine needle aspiration cytology rapid stain |
| url | https://jcdr.net/articles/PDF/9532/20882_CE[Ra]_F(Sh)_PF1(PI_VJ)_PFA(NC_AK)_PF2(NE_DK).pdf |
| work_keys_str_mv | AT prachisinkar utilityofmodifiedultrafastpapanicolaoustainincytologicaldiagnosis AT surekhaulhasarakeri utilityofmodifiedultrafastpapanicolaoustainincytologicaldiagnosis |