Inhibition Mechanism of Gosha-jinki-gan on the Micturition Reflex in Rats
We investigated the actions of Gosha-jinki-gan, a traditional Japanese medicine containing processed Aconiti tubers, on urinary bladder function in anesthetized rats. In cystometrical investigations, Gosha-jinki-gan (1.0 g/kg, i.d.) increased bladder capacity as well as micturition threshold pressur...
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| Format: | Article |
| Language: | English |
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Elsevier
2004-01-01
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| Series: | Journal of Pharmacological Sciences |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1347861319323539 |
| _version_ | 1831683376767041536 |
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| author | Akinobu Gotoh Kazuhiro Goto Atsushi Sengoku Toshiro Shirakawa Yoshinobu Akao Masato Fujisawa Hiroshi Okada Soichi Arakawa Sadao Kamidono |
| author_facet | Akinobu Gotoh Kazuhiro Goto Atsushi Sengoku Toshiro Shirakawa Yoshinobu Akao Masato Fujisawa Hiroshi Okada Soichi Arakawa Sadao Kamidono |
| author_sort | Akinobu Gotoh |
| collection | DOAJ |
| description | We investigated the actions of Gosha-jinki-gan, a traditional Japanese medicine containing processed Aconiti tubers, on urinary bladder function in anesthetized rats. In cystometrical investigations, Gosha-jinki-gan (1.0 g/kg, i.d.) increased bladder capacity as well as micturition threshold pressure. In addition, it decreased the frequency of distension-induced rhythmic bladder contractions. However, it did not influence the amplitude of bladder contractions induced by electrical stimulation of the pontine micturition center. The inhibitory effect of Gosha-jinki-gan on bladder motility was abolished by pretreatment with nor-binaltorphimine (10 mg/kg, s.c.), and was diminished by the concomitant use of anti-dynorphin A antiserum (10 μg, i.t.), yohimbine (10 μg, i.t.), or methysergide (20 μg, i.t.). Processed Aconiti tuber extract (27 mg/kg, i.d.) also suppressed bladder motility, and the effect was abolished by nor-binaltorphimine. These results suggest that Gosha-jinki-gan attenuates bladder sensation via the kappa-opioid receptor-stimulating action of processed Aconiti tuber. Gosha-jinki-gan may be a useful anti-pollakiuria agent that does not influence bladder contractility at micturition. Keywords:: Gosha-jinki-gan, anti-pollakiuria effect, micturition reflex, kappa-opioid receptor, processed Aconiti tuber |
| first_indexed | 2024-12-20T06:36:59Z |
| format | Article |
| id | doaj.art-4bd79fa1e0bc4dc4a99d357d2eb448b0 |
| institution | Directory Open Access Journal |
| issn | 1347-8613 |
| language | English |
| last_indexed | 2024-12-20T06:36:59Z |
| publishDate | 2004-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Pharmacological Sciences |
| spelling | doaj.art-4bd79fa1e0bc4dc4a99d357d2eb448b02022-12-21T19:49:59ZengElsevierJournal of Pharmacological Sciences1347-86132004-01-01962115123Inhibition Mechanism of Gosha-jinki-gan on the Micturition Reflex in RatsAkinobu Gotoh0Kazuhiro Goto1Atsushi Sengoku2Toshiro Shirakawa3Yoshinobu Akao4Masato Fujisawa5Hiroshi Okada6Soichi Arakawa7Sadao Kamidono8Division of Urology, Department of Organs Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanKyoto Regional Branch, Sales & Marketing Division, Tsumura & Co., 167 Dojisha-cho, Shimogyo-ku, Kyoto 600-8421, Japan; Corresponding author. FAX: +81-75-344-5018 E-mail: gotou_kazuhiro@mail.tsumura.co.jpDivision of Urology, Department of Organs Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDivision of Urology, Department of Organs Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDivision of Urology, Department of Organs Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDivision of Urology, Department of Organs Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDivision of Urology, Department of Organs Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDivision of Urology, Department of Organs Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanDivision of Urology, Department of Organs Therapeutics, Faculty of Medicine, Kobe University Graduate School of Medicine, 7-5-2 Kusunoki-cho, Chuo-ku, Kobe 650-0017, JapanWe investigated the actions of Gosha-jinki-gan, a traditional Japanese medicine containing processed Aconiti tubers, on urinary bladder function in anesthetized rats. In cystometrical investigations, Gosha-jinki-gan (1.0 g/kg, i.d.) increased bladder capacity as well as micturition threshold pressure. In addition, it decreased the frequency of distension-induced rhythmic bladder contractions. However, it did not influence the amplitude of bladder contractions induced by electrical stimulation of the pontine micturition center. The inhibitory effect of Gosha-jinki-gan on bladder motility was abolished by pretreatment with nor-binaltorphimine (10 mg/kg, s.c.), and was diminished by the concomitant use of anti-dynorphin A antiserum (10 μg, i.t.), yohimbine (10 μg, i.t.), or methysergide (20 μg, i.t.). Processed Aconiti tuber extract (27 mg/kg, i.d.) also suppressed bladder motility, and the effect was abolished by nor-binaltorphimine. These results suggest that Gosha-jinki-gan attenuates bladder sensation via the kappa-opioid receptor-stimulating action of processed Aconiti tuber. Gosha-jinki-gan may be a useful anti-pollakiuria agent that does not influence bladder contractility at micturition. Keywords:: Gosha-jinki-gan, anti-pollakiuria effect, micturition reflex, kappa-opioid receptor, processed Aconiti tuberhttp://www.sciencedirect.com/science/article/pii/S1347861319323539 |
| spellingShingle | Akinobu Gotoh Kazuhiro Goto Atsushi Sengoku Toshiro Shirakawa Yoshinobu Akao Masato Fujisawa Hiroshi Okada Soichi Arakawa Sadao Kamidono Inhibition Mechanism of Gosha-jinki-gan on the Micturition Reflex in Rats Journal of Pharmacological Sciences |
| title | Inhibition Mechanism of Gosha-jinki-gan on the Micturition Reflex in Rats |
| title_full | Inhibition Mechanism of Gosha-jinki-gan on the Micturition Reflex in Rats |
| title_fullStr | Inhibition Mechanism of Gosha-jinki-gan on the Micturition Reflex in Rats |
| title_full_unstemmed | Inhibition Mechanism of Gosha-jinki-gan on the Micturition Reflex in Rats |
| title_short | Inhibition Mechanism of Gosha-jinki-gan on the Micturition Reflex in Rats |
| title_sort | inhibition mechanism of gosha jinki gan on the micturition reflex in rats |
| url | http://www.sciencedirect.com/science/article/pii/S1347861319323539 |
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