The Higher Inherent Therapeutic Potential of Biomaterial-Based hDPSCs and hEnSCs for Pancreas Diseases
Human endometrial stem cells (hEnSCs), dental pulp stem cells (hDPSCs) and adipose tissue-derived stem cells (hADSCs) are considered to be the promising candidates for the treatment of pancreas diseases. The prognosis is better with in situ injection of mesenchymal stem cells (MSCs) to the damaged p...
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| Format: | Article |
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Frontiers Media S.A.
2020-06-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fbioe.2020.00636/full |
| _version_ | 1819229760140607488 |
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| author | Bingbing Xu Fu-Zhen Yuan Lin Lin Jing Ye Bao-Shi Fan Ji-Ying Zhang Meng Yang Dong Jiang Wen-Bo Jiang Xing Wang Xing Wang Jia-Kuo Yu |
| author_facet | Bingbing Xu Fu-Zhen Yuan Lin Lin Jing Ye Bao-Shi Fan Ji-Ying Zhang Meng Yang Dong Jiang Wen-Bo Jiang Xing Wang Xing Wang Jia-Kuo Yu |
| author_sort | Bingbing Xu |
| collection | DOAJ |
| description | Human endometrial stem cells (hEnSCs), dental pulp stem cells (hDPSCs) and adipose tissue-derived stem cells (hADSCs) are considered to be the promising candidates for the treatment of pancreas diseases. The prognosis is better with in situ injection of mesenchymal stem cells (MSCs) to the damaged pancreas compared with intravenous injection. However, the clinical application of these cells are limited, due to poor engraftment of transplanted cells after delivery. On the other hand, understanding the role of the biomaterials in cell therapy is essential to promote the therapeutic effects of MSCs. Matrigel, a basement membrane matrix biomaterial, is rich in laminin and collagen IV. The aim of this study is to investigate the difference of biological characteristics of hEnSCs, hDPSCs and hADSCs in vitro and their survival situation with Matrigel post intrapancreatic transplantation in vivo. Our findings showed, firstly, there was no significant difference in morphology and immunophenotype of these MSCs. Secondly, the biological properties, including cell proliferation, the ability of adipogenic and osteogenic differentiation and the mRNA expression levels of pancreas development-related genes, have been showed distinct difference among these MSCs. Thirdly, Matrigel can improve the survival of MSCs in vivo, especially for Matrigel-based hDPSCs and Matrigel-based hEnSCs in pancreas parenchyma of SD rats. These results suggest that hDPSCs and hEnSCs are with the greater inherent therapeutic potential for pancreas diseases compared with hADSCs. |
| first_indexed | 2024-12-23T11:18:17Z |
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| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-12-23T11:18:17Z |
| publishDate | 2020-06-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-4bd998ab9b634db990ff31b933f05e0a2022-12-21T17:49:08ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-06-01810.3389/fbioe.2020.00636550512The Higher Inherent Therapeutic Potential of Biomaterial-Based hDPSCs and hEnSCs for Pancreas DiseasesBingbing Xu0Fu-Zhen Yuan1Lin Lin2Jing Ye3Bao-Shi Fan4Ji-Ying Zhang5Meng Yang6Dong Jiang7Wen-Bo Jiang8Xing Wang9Xing Wang10Jia-Kuo Yu11Knee Surgery Department of the Institute of Sports Medicine, Peking University Third Hospital, Beijing, ChinaKnee Surgery Department of the Institute of Sports Medicine, Peking University Third Hospital, Beijing, ChinaKnee Surgery Department of the Institute of Sports Medicine, Peking University Third Hospital, Beijing, ChinaKnee Surgery Department of the Institute of Sports Medicine, Peking University Third Hospital, Beijing, ChinaSchool of Clinical Medicine, Weifang Medical University, Weifang, ChinaKnee Surgery Department of the Institute of Sports Medicine, Peking University Third Hospital, Beijing, ChinaSchool of Clinical Medicine, Weifang Medical University, Weifang, ChinaKnee Surgery Department of the Institute of Sports Medicine, Peking University Third Hospital, Beijing, ChinaClinical Translational R&D Center of 3D Printing Technology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaBeijing National Laboratory for Molecular Sciences, State Key Laboratory of Polymer Physics & Chemistry, Institute of Chemistry, Chinese Academy of Sciences, Beijing, ChinaUniversity of Chinese Academy of Sciences, Beijing, ChinaKnee Surgery Department of the Institute of Sports Medicine, Peking University Third Hospital, Beijing, ChinaHuman endometrial stem cells (hEnSCs), dental pulp stem cells (hDPSCs) and adipose tissue-derived stem cells (hADSCs) are considered to be the promising candidates for the treatment of pancreas diseases. The prognosis is better with in situ injection of mesenchymal stem cells (MSCs) to the damaged pancreas compared with intravenous injection. However, the clinical application of these cells are limited, due to poor engraftment of transplanted cells after delivery. On the other hand, understanding the role of the biomaterials in cell therapy is essential to promote the therapeutic effects of MSCs. Matrigel, a basement membrane matrix biomaterial, is rich in laminin and collagen IV. The aim of this study is to investigate the difference of biological characteristics of hEnSCs, hDPSCs and hADSCs in vitro and their survival situation with Matrigel post intrapancreatic transplantation in vivo. Our findings showed, firstly, there was no significant difference in morphology and immunophenotype of these MSCs. Secondly, the biological properties, including cell proliferation, the ability of adipogenic and osteogenic differentiation and the mRNA expression levels of pancreas development-related genes, have been showed distinct difference among these MSCs. Thirdly, Matrigel can improve the survival of MSCs in vivo, especially for Matrigel-based hDPSCs and Matrigel-based hEnSCs in pancreas parenchyma of SD rats. These results suggest that hDPSCs and hEnSCs are with the greater inherent therapeutic potential for pancreas diseases compared with hADSCs.https://www.frontiersin.org/article/10.3389/fbioe.2020.00636/fullhEnSCshDPSCshADSCsbiomaterialorthotopic transplantation |
| spellingShingle | Bingbing Xu Fu-Zhen Yuan Lin Lin Jing Ye Bao-Shi Fan Ji-Ying Zhang Meng Yang Dong Jiang Wen-Bo Jiang Xing Wang Xing Wang Jia-Kuo Yu The Higher Inherent Therapeutic Potential of Biomaterial-Based hDPSCs and hEnSCs for Pancreas Diseases Frontiers in Bioengineering and Biotechnology hEnSCs hDPSCs hADSCs biomaterial orthotopic transplantation |
| title | The Higher Inherent Therapeutic Potential of Biomaterial-Based hDPSCs and hEnSCs for Pancreas Diseases |
| title_full | The Higher Inherent Therapeutic Potential of Biomaterial-Based hDPSCs and hEnSCs for Pancreas Diseases |
| title_fullStr | The Higher Inherent Therapeutic Potential of Biomaterial-Based hDPSCs and hEnSCs for Pancreas Diseases |
| title_full_unstemmed | The Higher Inherent Therapeutic Potential of Biomaterial-Based hDPSCs and hEnSCs for Pancreas Diseases |
| title_short | The Higher Inherent Therapeutic Potential of Biomaterial-Based hDPSCs and hEnSCs for Pancreas Diseases |
| title_sort | higher inherent therapeutic potential of biomaterial based hdpscs and henscs for pancreas diseases |
| topic | hEnSCs hDPSCs hADSCs biomaterial orthotopic transplantation |
| url | https://www.frontiersin.org/article/10.3389/fbioe.2020.00636/full |
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