Neutral glycolipids in leukemic and nonleukemic leukocytes

Neutral lipids, free and total cholesterol, glycolipids, and phospholipids were determined in 20 preparations of leukocytes distributed in four groups. Group I consisted of leukocytes from nonleukemic patients; group II, from patients with chronic myelogenous leukemia; group III, from patients with...

Full description

Bibliographic Details
Main Authors: J. HILDEBRAND, P. STRYCKMANS, P. STOFFYN
Format: Article
Language:English
Published: Elsevier 1971-05-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227520395171
_version_ 1818590523371290624
author J. HILDEBRAND
P. STRYCKMANS
P. STOFFYN
author_facet J. HILDEBRAND
P. STRYCKMANS
P. STOFFYN
author_sort J. HILDEBRAND
collection DOAJ
description Neutral lipids, free and total cholesterol, glycolipids, and phospholipids were determined in 20 preparations of leukocytes distributed in four groups. Group I consisted of leukocytes from nonleukemic patients; group II, from patients with chronic myelogenous leukemia; group III, from patients with chronic lymphocytic leukemia; and group IV, from patients with acute leukemia.Two neutral glycolipids were found in nonleukemic mixed leukocyte populations. They were identified as glucosylceramide and lactosylceramide. The same glycolipids were also present in leukemic cells, but striking differences in glycolipid composition were found in various types of leukocytes.Glycolipids accounted for 8.9–12.6% of the total lipids in leukocytes from group I, 11.4–20.4% in group II, 1.2–1.6% in group III, and 0.5–4.9% in group IV.Glucosylceramide was the only glycolipid found in seven out of eight analyzed samples of lymphocytes, both normal and leukemic. Lactosylceramide was the major glycolipid in preparations consisting mainly of polymorphonuclear, myeloid, and blastic cells. Only lactosylceramide was found in platelets, where its concentration was about 100 times lower than in mixed leukocyte populations.
first_indexed 2024-12-16T09:57:54Z
format Article
id doaj.art-4bdb6f0f6b75417bbd5b60cfe0c3044c
institution Directory Open Access Journal
issn 0022-2275
language English
last_indexed 2024-12-16T09:57:54Z
publishDate 1971-05-01
publisher Elsevier
record_format Article
series Journal of Lipid Research
spelling doaj.art-4bdb6f0f6b75417bbd5b60cfe0c3044c2022-12-21T22:35:52ZengElsevierJournal of Lipid Research0022-22751971-05-01123361366Neutral glycolipids in leukemic and nonleukemic leukocytesJ. HILDEBRAND0P. STRYCKMANS1P. STOFFYN2Department of Internal Medicine and Clinical Investigation, Institut Jules Bordet, Brussels University, Brussels, BelgiumDepartment of Internal Medicine and Clinical Investigation, Institut Jules Bordet, Brussels University, Brussels, BelgiumDepartment of Internal Medicine and Clinical Investigation, Institut Jules Bordet, Brussels University, Brussels, BelgiumNeutral lipids, free and total cholesterol, glycolipids, and phospholipids were determined in 20 preparations of leukocytes distributed in four groups. Group I consisted of leukocytes from nonleukemic patients; group II, from patients with chronic myelogenous leukemia; group III, from patients with chronic lymphocytic leukemia; and group IV, from patients with acute leukemia.Two neutral glycolipids were found in nonleukemic mixed leukocyte populations. They were identified as glucosylceramide and lactosylceramide. The same glycolipids were also present in leukemic cells, but striking differences in glycolipid composition were found in various types of leukocytes.Glycolipids accounted for 8.9–12.6% of the total lipids in leukocytes from group I, 11.4–20.4% in group II, 1.2–1.6% in group III, and 0.5–4.9% in group IV.Glucosylceramide was the only glycolipid found in seven out of eight analyzed samples of lymphocytes, both normal and leukemic. Lactosylceramide was the major glycolipid in preparations consisting mainly of polymorphonuclear, myeloid, and blastic cells. Only lactosylceramide was found in platelets, where its concentration was about 100 times lower than in mixed leukocyte populations.http://www.sciencedirect.com/science/article/pii/S0022227520395171glucosylceramidelactosylceramide
spellingShingle J. HILDEBRAND
P. STRYCKMANS
P. STOFFYN
Neutral glycolipids in leukemic and nonleukemic leukocytes
Journal of Lipid Research
glucosylceramide
lactosylceramide
title Neutral glycolipids in leukemic and nonleukemic leukocytes
title_full Neutral glycolipids in leukemic and nonleukemic leukocytes
title_fullStr Neutral glycolipids in leukemic and nonleukemic leukocytes
title_full_unstemmed Neutral glycolipids in leukemic and nonleukemic leukocytes
title_short Neutral glycolipids in leukemic and nonleukemic leukocytes
title_sort neutral glycolipids in leukemic and nonleukemic leukocytes
topic glucosylceramide
lactosylceramide
url http://www.sciencedirect.com/science/article/pii/S0022227520395171
work_keys_str_mv AT jhildebrand neutralglycolipidsinleukemicandnonleukemicleukocytes
AT pstryckmans neutralglycolipidsinleukemicandnonleukemicleukocytes
AT pstoffyn neutralglycolipidsinleukemicandnonleukemicleukocytes