Neutral glycolipids in leukemic and nonleukemic leukocytes
Neutral lipids, free and total cholesterol, glycolipids, and phospholipids were determined in 20 preparations of leukocytes distributed in four groups. Group I consisted of leukocytes from nonleukemic patients; group II, from patients with chronic myelogenous leukemia; group III, from patients with...
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Format: | Article |
Language: | English |
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Elsevier
1971-05-01
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Series: | Journal of Lipid Research |
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Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520395171 |
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author | J. HILDEBRAND P. STRYCKMANS P. STOFFYN |
author_facet | J. HILDEBRAND P. STRYCKMANS P. STOFFYN |
author_sort | J. HILDEBRAND |
collection | DOAJ |
description | Neutral lipids, free and total cholesterol, glycolipids, and phospholipids were determined in 20 preparations of leukocytes distributed in four groups. Group I consisted of leukocytes from nonleukemic patients; group II, from patients with chronic myelogenous leukemia; group III, from patients with chronic lymphocytic leukemia; and group IV, from patients with acute leukemia.Two neutral glycolipids were found in nonleukemic mixed leukocyte populations. They were identified as glucosylceramide and lactosylceramide. The same glycolipids were also present in leukemic cells, but striking differences in glycolipid composition were found in various types of leukocytes.Glycolipids accounted for 8.9–12.6% of the total lipids in leukocytes from group I, 11.4–20.4% in group II, 1.2–1.6% in group III, and 0.5–4.9% in group IV.Glucosylceramide was the only glycolipid found in seven out of eight analyzed samples of lymphocytes, both normal and leukemic. Lactosylceramide was the major glycolipid in preparations consisting mainly of polymorphonuclear, myeloid, and blastic cells. Only lactosylceramide was found in platelets, where its concentration was about 100 times lower than in mixed leukocyte populations. |
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issn | 0022-2275 |
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spelling | doaj.art-4bdb6f0f6b75417bbd5b60cfe0c3044c2022-12-21T22:35:52ZengElsevierJournal of Lipid Research0022-22751971-05-01123361366Neutral glycolipids in leukemic and nonleukemic leukocytesJ. HILDEBRAND0P. STRYCKMANS1P. STOFFYN2Department of Internal Medicine and Clinical Investigation, Institut Jules Bordet, Brussels University, Brussels, BelgiumDepartment of Internal Medicine and Clinical Investigation, Institut Jules Bordet, Brussels University, Brussels, BelgiumDepartment of Internal Medicine and Clinical Investigation, Institut Jules Bordet, Brussels University, Brussels, BelgiumNeutral lipids, free and total cholesterol, glycolipids, and phospholipids were determined in 20 preparations of leukocytes distributed in four groups. Group I consisted of leukocytes from nonleukemic patients; group II, from patients with chronic myelogenous leukemia; group III, from patients with chronic lymphocytic leukemia; and group IV, from patients with acute leukemia.Two neutral glycolipids were found in nonleukemic mixed leukocyte populations. They were identified as glucosylceramide and lactosylceramide. The same glycolipids were also present in leukemic cells, but striking differences in glycolipid composition were found in various types of leukocytes.Glycolipids accounted for 8.9–12.6% of the total lipids in leukocytes from group I, 11.4–20.4% in group II, 1.2–1.6% in group III, and 0.5–4.9% in group IV.Glucosylceramide was the only glycolipid found in seven out of eight analyzed samples of lymphocytes, both normal and leukemic. Lactosylceramide was the major glycolipid in preparations consisting mainly of polymorphonuclear, myeloid, and blastic cells. Only lactosylceramide was found in platelets, where its concentration was about 100 times lower than in mixed leukocyte populations.http://www.sciencedirect.com/science/article/pii/S0022227520395171glucosylceramidelactosylceramide |
spellingShingle | J. HILDEBRAND P. STRYCKMANS P. STOFFYN Neutral glycolipids in leukemic and nonleukemic leukocytes Journal of Lipid Research glucosylceramide lactosylceramide |
title | Neutral glycolipids in leukemic and nonleukemic leukocytes |
title_full | Neutral glycolipids in leukemic and nonleukemic leukocytes |
title_fullStr | Neutral glycolipids in leukemic and nonleukemic leukocytes |
title_full_unstemmed | Neutral glycolipids in leukemic and nonleukemic leukocytes |
title_short | Neutral glycolipids in leukemic and nonleukemic leukocytes |
title_sort | neutral glycolipids in leukemic and nonleukemic leukocytes |
topic | glucosylceramide lactosylceramide |
url | http://www.sciencedirect.com/science/article/pii/S0022227520395171 |
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