ANALYSIS OF SPERMATOZOA QUALITY USING PERCOLL DENSITY GRADIENT CENTRIFUGATION THROUGH THE ADMINISTRATION OF PHOSPHOLIPID + EGTA
The Percoll density gradient centrifugation (PDGC) method is frequently used in the sexing of spermatozoa. However, this method causes damage to the spermatozoa membranes, resulting in a decreased quality of spermatozoa. We analysed the impacts of phospholipid PC (phosphatidylcholine) and EGTA (ethy...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Iasi University of Life Sciences
2022-02-01
|
| Series: | Journal of Applied Life Sciences and Environment |
| Subjects: | |
| Online Access: | https://jurnalalse.com/wp-content/uploads/JALSE3-21-06.pdf |
| _version_ | 1797667168493502464 |
|---|---|
| author | ROOIJE R.H. RUMENDE EVA L. BAIDENG FREDINE E.S. RARES LAYA M. RARES |
| author_facet | ROOIJE R.H. RUMENDE EVA L. BAIDENG FREDINE E.S. RARES LAYA M. RARES |
| author_sort | ROOIJE R.H. RUMENDE |
| collection | DOAJ |
| description | The Percoll density gradient centrifugation (PDGC) method is frequently used in the sexing of spermatozoa. However, this method causes damage to the spermatozoa membranes, resulting in a decreased quality of spermatozoa. We analysed the impacts of phospholipid PC (phosphatidylcholine) and EGTA (ethylene glycol bis (β-aminoethyl ether) N,N,N’,N’-tetraacetic acid) Ca2+ free buffer on the quality of bovine spermatozoa after the PDGC process, using semen from Friesian Holstein (FH) bulls aged 5 – 8 years. The following variables were observed: spermatozoa motility, spermatozoa viability, spermatozoa membrane integrity, spermatozoa that have not experienced capacitation, spermatozoa that have experienced capacitation and spermatozoa that have undergone acrosomal reaction. The results showed that the administration of phospholipid PC + EGTA Ca2+ free buffer to spermatozoa, followed by the PDGC process, could maintain or further improve the values of all variables. In the PDGC process, phospholipid PC 10% + EGTA Ca2+ free buffer at 1 mM was most suitable. |
| first_indexed | 2024-03-11T20:10:18Z |
| format | Article |
| id | doaj.art-4bde096cfb624ec6b4929af565de41c2 |
| institution | Directory Open Access Journal |
| issn | 2784-0379 2784-0360 |
| language | English |
| last_indexed | 2024-03-11T20:10:18Z |
| publishDate | 2022-02-01 |
| publisher | Iasi University of Life Sciences |
| record_format | Article |
| series | Journal of Applied Life Sciences and Environment |
| spelling | doaj.art-4bde096cfb624ec6b4929af565de41c22023-10-03T11:59:22ZengIasi University of Life SciencesJournal of Applied Life Sciences and Environment2784-03792784-03602022-02-01543(187)298309https://doi.org/10.46909//journalalse-2021-026ANALYSIS OF SPERMATOZOA QUALITY USING PERCOLL DENSITY GRADIENT CENTRIFUGATION THROUGH THE ADMINISTRATION OF PHOSPHOLIPID + EGTAROOIJE R.H. RUMENDE0EVA L. BAIDENG1FREDINE E.S. RARES2LAYA M. RARES3Department of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado, IndonesiaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Sam Ratulangi University, Manado, IndonesiaSection of Microbiology, Faculty of Medicine, Sam Ratulangi University, Manado, IndonesiaSection of Ophthalmology, Faculty of Medicine, Sam Ratulangi University, Manado, IndonesiaThe Percoll density gradient centrifugation (PDGC) method is frequently used in the sexing of spermatozoa. However, this method causes damage to the spermatozoa membranes, resulting in a decreased quality of spermatozoa. We analysed the impacts of phospholipid PC (phosphatidylcholine) and EGTA (ethylene glycol bis (β-aminoethyl ether) N,N,N’,N’-tetraacetic acid) Ca2+ free buffer on the quality of bovine spermatozoa after the PDGC process, using semen from Friesian Holstein (FH) bulls aged 5 – 8 years. The following variables were observed: spermatozoa motility, spermatozoa viability, spermatozoa membrane integrity, spermatozoa that have not experienced capacitation, spermatozoa that have experienced capacitation and spermatozoa that have undergone acrosomal reaction. The results showed that the administration of phospholipid PC + EGTA Ca2+ free buffer to spermatozoa, followed by the PDGC process, could maintain or further improve the values of all variables. In the PDGC process, phospholipid PC 10% + EGTA Ca2+ free buffer at 1 mM was most suitable.https://jurnalalse.com/wp-content/uploads/JALSE3-21-06.pdfpdgcpc phospholipidsegtaseparation spermatozoa |
| spellingShingle | ROOIJE R.H. RUMENDE EVA L. BAIDENG FREDINE E.S. RARES LAYA M. RARES ANALYSIS OF SPERMATOZOA QUALITY USING PERCOLL DENSITY GRADIENT CENTRIFUGATION THROUGH THE ADMINISTRATION OF PHOSPHOLIPID + EGTA Journal of Applied Life Sciences and Environment pdgc pc phospholipids egta separation spermatozoa |
| title | ANALYSIS OF SPERMATOZOA QUALITY USING PERCOLL DENSITY GRADIENT CENTRIFUGATION THROUGH THE ADMINISTRATION OF PHOSPHOLIPID + EGTA |
| title_full | ANALYSIS OF SPERMATOZOA QUALITY USING PERCOLL DENSITY GRADIENT CENTRIFUGATION THROUGH THE ADMINISTRATION OF PHOSPHOLIPID + EGTA |
| title_fullStr | ANALYSIS OF SPERMATOZOA QUALITY USING PERCOLL DENSITY GRADIENT CENTRIFUGATION THROUGH THE ADMINISTRATION OF PHOSPHOLIPID + EGTA |
| title_full_unstemmed | ANALYSIS OF SPERMATOZOA QUALITY USING PERCOLL DENSITY GRADIENT CENTRIFUGATION THROUGH THE ADMINISTRATION OF PHOSPHOLIPID + EGTA |
| title_short | ANALYSIS OF SPERMATOZOA QUALITY USING PERCOLL DENSITY GRADIENT CENTRIFUGATION THROUGH THE ADMINISTRATION OF PHOSPHOLIPID + EGTA |
| title_sort | analysis of spermatozoa quality using percoll density gradient centrifugation through the administration of phospholipid egta |
| topic | pdgc pc phospholipids egta separation spermatozoa |
| url | https://jurnalalse.com/wp-content/uploads/JALSE3-21-06.pdf |
| work_keys_str_mv | AT rooijerhrumende analysisofspermatozoaqualityusingpercolldensitygradientcentrifugationthroughtheadministrationofphospholipidegta AT evalbaideng analysisofspermatozoaqualityusingpercolldensitygradientcentrifugationthroughtheadministrationofphospholipidegta AT fredineesrares analysisofspermatozoaqualityusingpercolldensitygradientcentrifugationthroughtheadministrationofphospholipidegta AT layamrares analysisofspermatozoaqualityusingpercolldensitygradientcentrifugationthroughtheadministrationofphospholipidegta |