Causal role for the subthalamic nucleus in interrupting behavior
Stopping or pausing in response to threats, conflicting information, or surprise is fundamental to behavior. Evidence across species has shown that the subthalamic nucleus (STN) is activated by scenarios involving stopping or pausing, yet evidence that the STN causally implements stops or pauses is...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-07-01
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| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/27689 |
| _version_ | 1811201591802331136 |
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| author | Kathryn H Fife Navarre A Gutierrez-Reed Vivien Zell Julie Bailly Christina M Lewis Adam R Aron Thomas S Hnasko |
| author_facet | Kathryn H Fife Navarre A Gutierrez-Reed Vivien Zell Julie Bailly Christina M Lewis Adam R Aron Thomas S Hnasko |
| author_sort | Kathryn H Fife |
| collection | DOAJ |
| description | Stopping or pausing in response to threats, conflicting information, or surprise is fundamental to behavior. Evidence across species has shown that the subthalamic nucleus (STN) is activated by scenarios involving stopping or pausing, yet evidence that the STN causally implements stops or pauses is lacking. Here we used optogenetics to activate or inhibit mouse STN to test its putative causal role. We first demonstrated that optogenetic stimulation of the STN excited its major projection targets. Next we showed that brief activation of STN projection neurons was sufficient to interrupt or pause a self-initiated bout of licking. Finally, we developed an assay in which surprise was used to interrupt licking, and showed that STN inhibition reduced the disruptive effect of surprise. Thus STN activation interrupts behavior, and blocking the STN blunts the interruptive effect of surprise. These results provide strong evidence that the STN is both necessary and sufficient for such forms of behavioral response suppression. |
| first_indexed | 2024-04-12T02:24:15Z |
| format | Article |
| id | doaj.art-4bdeaedee74442f7bbc007732fc6acc9 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T02:24:15Z |
| publishDate | 2017-07-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-4bdeaedee74442f7bbc007732fc6acc92022-12-22T03:52:02ZengeLife Sciences Publications LtdeLife2050-084X2017-07-01610.7554/eLife.27689Causal role for the subthalamic nucleus in interrupting behaviorKathryn H Fife0Navarre A Gutierrez-Reed1Vivien Zell2Julie Bailly3Christina M Lewis4Adam R Aron5Thomas S Hnasko6https://orcid.org/0000-0001-6176-8513Neurosciences Graduate Program, University of California, San Diego, San Diego, United StatesBiomedical Sciences Graduate Program, University of California, San Diego, San Diego, United StatesDepartment of Neurosciences, University of California, San Diego, San Diego, United StatesDepartment of Neurosciences, University of California, San Diego, San Diego, United StatesDepartment of Psychology, University of California, San Diego, San Diego, United StatesDepartment of Psychology, University of California, San Diego, San Diego, United StatesDepartment of Neurosciences, University of California, San Diego, San Diego, United StatesStopping or pausing in response to threats, conflicting information, or surprise is fundamental to behavior. Evidence across species has shown that the subthalamic nucleus (STN) is activated by scenarios involving stopping or pausing, yet evidence that the STN causally implements stops or pauses is lacking. Here we used optogenetics to activate or inhibit mouse STN to test its putative causal role. We first demonstrated that optogenetic stimulation of the STN excited its major projection targets. Next we showed that brief activation of STN projection neurons was sufficient to interrupt or pause a self-initiated bout of licking. Finally, we developed an assay in which surprise was used to interrupt licking, and showed that STN inhibition reduced the disruptive effect of surprise. Thus STN activation interrupts behavior, and blocking the STN blunts the interruptive effect of surprise. These results provide strong evidence that the STN is both necessary and sufficient for such forms of behavioral response suppression.https://elifesciences.org/articles/27689subthalamic nucleusSTNresponse inhibitionstoppingoptogeneticsvesicular glutamate transporter 2 |
| spellingShingle | Kathryn H Fife Navarre A Gutierrez-Reed Vivien Zell Julie Bailly Christina M Lewis Adam R Aron Thomas S Hnasko Causal role for the subthalamic nucleus in interrupting behavior eLife subthalamic nucleus STN response inhibition stopping optogenetics vesicular glutamate transporter 2 |
| title | Causal role for the subthalamic nucleus in interrupting behavior |
| title_full | Causal role for the subthalamic nucleus in interrupting behavior |
| title_fullStr | Causal role for the subthalamic nucleus in interrupting behavior |
| title_full_unstemmed | Causal role for the subthalamic nucleus in interrupting behavior |
| title_short | Causal role for the subthalamic nucleus in interrupting behavior |
| title_sort | causal role for the subthalamic nucleus in interrupting behavior |
| topic | subthalamic nucleus STN response inhibition stopping optogenetics vesicular glutamate transporter 2 |
| url | https://elifesciences.org/articles/27689 |
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