<i>In vitro</i> activity of human recombinant alpha-2b interferon against SARS-CoV-2 virus
Introduction. The pandemic spread of a new coronavirus infection, COVID-19, has caused a global emergency and attracted the attention of public health professionals and the population of all countries. A significant increase in the number of new cases of SARS-CoV-2 infection demonstrates the urgency...
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| Format: | Article |
| Language: | English |
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Central Research Institute for Epidemiology
2021-05-01
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| Series: | Вопросы вирусологии |
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| Online Access: | https://virusjour.crie.ru/jour/article/viewFile/496/311 |
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| author | S. Ya. Loginova V. N. Shсhukina S. V. Savenko S. V. Borisevich |
| author_facet | S. Ya. Loginova V. N. Shсhukina S. V. Savenko S. V. Borisevich |
| author_sort | S. Ya. Loginova |
| collection | DOAJ |
| description | Introduction. The pandemic spread of a new coronavirus infection, COVID-19, has caused a global emergency and attracted the attention of public health professionals and the population of all countries. A significant increase in the number of new cases of SARS-CoV-2 infection demonstrates the urgency of finding drugs effective against this pathogen.
The aim of this work was to evaluate the in vitro antiviral efficacy of human recombinant alpha-2b interferon (IFN-α2b) against SARS-CoV-2 virus.
Material and methods. The experiments had been carried out on Vero Cl008, the continuous line of African green monkey (Chlorocebus sabaeus) kidney cells. The effectiveness of the drugs was assessed by the suppression of viral reproduction in vitro. The biological activity was determined using titration of a virus-containing suspension in a Vero Cl008 cell culture by the formation of negative colonies.
Results. The antiviral efficacy of the IFN-α2b-based medications, which have a high safety profile and proven efficacy in the prevention and treatment of influenza and acute respiratory viral infections (ARVI), has been studied against the new pandemic SARS-CoV-2 virus in vitro experiments in Vero C1008 cell culture. IFN-α2b effectively inhibits the reproduction of the virus when applied both 24 hrs before and 2 hrs after infection. In the IFN-α2b concentration range 102–106 IU/ml a complete suppression of the reproduction of the SARS-CoV-2 virus had been demonstrated.
Discussion. IFN-α2b demonstrated in vitro high antiviral activity against SARS-CoV-2. In addition, the substance has a high chemotherapeutic index (1000).
Conclusion. Medications for intranasal use based on IFN-α2b have high antiviral activity and are promising drugs for in vivo study in terms of prevention and treatment of COVID-19. |
| first_indexed | 2024-03-13T00:08:34Z |
| format | Article |
| id | doaj.art-4bdf811751c3433e95a24dc90399e2de |
| institution | Directory Open Access Journal |
| issn | 0507-4088 2411-2097 |
| language | English |
| last_indexed | 2024-03-13T00:08:34Z |
| publishDate | 2021-05-01 |
| publisher | Central Research Institute for Epidemiology |
| record_format | Article |
| series | Вопросы вирусологии |
| spelling | doaj.art-4bdf811751c3433e95a24dc90399e2de2023-07-12T19:56:47ZengCentral Research Institute for EpidemiologyВопросы вирусологии0507-40882411-20972021-05-0166212312810.36233/0507-4088-13379<i>In vitro</i> activity of human recombinant alpha-2b interferon against SARS-CoV-2 virusS. Ya. Loginova0https://orcid.org/0000-0001-6732-8404V. N. Shсhukina1https://orcid.org/0000-0002-5461-3641S. V. Savenko2https://orcid.org/0000-0002-5175-916XS. V. Borisevich3https://orcid.org/0000-0002-6742-3919FSBI «Central Scientific Research Institute No. 48» of the Ministry of Defense of RussiaFSBI «Central Scientific Research Institute No. 48» of the Ministry of Defense of RussiaFSBI «Central Scientific Research Institute No. 48» of the Ministry of Defense of RussiaFSBI «Central Scientific Research Institute No. 48» of the Ministry of Defense of RussiaIntroduction. The pandemic spread of a new coronavirus infection, COVID-19, has caused a global emergency and attracted the attention of public health professionals and the population of all countries. A significant increase in the number of new cases of SARS-CoV-2 infection demonstrates the urgency of finding drugs effective against this pathogen. The aim of this work was to evaluate the in vitro antiviral efficacy of human recombinant alpha-2b interferon (IFN-α2b) against SARS-CoV-2 virus. Material and methods. The experiments had been carried out on Vero Cl008, the continuous line of African green monkey (Chlorocebus sabaeus) kidney cells. The effectiveness of the drugs was assessed by the suppression of viral reproduction in vitro. The biological activity was determined using titration of a virus-containing suspension in a Vero Cl008 cell culture by the formation of negative colonies. Results. The antiviral efficacy of the IFN-α2b-based medications, which have a high safety profile and proven efficacy in the prevention and treatment of influenza and acute respiratory viral infections (ARVI), has been studied against the new pandemic SARS-CoV-2 virus in vitro experiments in Vero C1008 cell culture. IFN-α2b effectively inhibits the reproduction of the virus when applied both 24 hrs before and 2 hrs after infection. In the IFN-α2b concentration range 102–106 IU/ml a complete suppression of the reproduction of the SARS-CoV-2 virus had been demonstrated. Discussion. IFN-α2b demonstrated in vitro high antiviral activity against SARS-CoV-2. In addition, the substance has a high chemotherapeutic index (1000). Conclusion. Medications for intranasal use based on IFN-α2b have high antiviral activity and are promising drugs for in vivo study in terms of prevention and treatment of COVID-19.https://virusjour.crie.ru/jour/article/viewFile/496/311interferon α2bcovid-19sars-cov-2veroin vitroantiviral activity |
| spellingShingle | S. Ya. Loginova V. N. Shсhukina S. V. Savenko S. V. Borisevich <i>In vitro</i> activity of human recombinant alpha-2b interferon against SARS-CoV-2 virus Вопросы вирусологии interferon α2b covid-19 sars-cov-2 vero in vitro antiviral activity |
| title | <i>In vitro</i> activity of human recombinant alpha-2b interferon against SARS-CoV-2 virus |
| title_full | <i>In vitro</i> activity of human recombinant alpha-2b interferon against SARS-CoV-2 virus |
| title_fullStr | <i>In vitro</i> activity of human recombinant alpha-2b interferon against SARS-CoV-2 virus |
| title_full_unstemmed | <i>In vitro</i> activity of human recombinant alpha-2b interferon against SARS-CoV-2 virus |
| title_short | <i>In vitro</i> activity of human recombinant alpha-2b interferon against SARS-CoV-2 virus |
| title_sort | i in vitro i activity of human recombinant alpha 2b interferon against sars cov 2 virus |
| topic | interferon α2b covid-19 sars-cov-2 vero in vitro antiviral activity |
| url | https://virusjour.crie.ru/jour/article/viewFile/496/311 |
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