Homologous and heterologous protection of nonhuman primates by Ebola and Sudan virus-like particles.
Filoviruses cause hemorrhagic fever resulting in significant morbidity and mortality in humans. Several vaccine platforms that include multiple virus-vectored approaches and virus-like particles (VLPs) have shown efficacy in nonhuman primates. Previous studies have shown protection of cynomolgus mac...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0118881 |
| _version_ | 1818835334883966976 |
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| author | Kelly L Warfield John M Dye Jay B Wells Robert C Unfer Frederick W Holtsberg Sergey Shulenin Hong Vu Dana L Swenson Sina Bavari M Javad Aman |
| author_facet | Kelly L Warfield John M Dye Jay B Wells Robert C Unfer Frederick W Holtsberg Sergey Shulenin Hong Vu Dana L Swenson Sina Bavari M Javad Aman |
| author_sort | Kelly L Warfield |
| collection | DOAJ |
| description | Filoviruses cause hemorrhagic fever resulting in significant morbidity and mortality in humans. Several vaccine platforms that include multiple virus-vectored approaches and virus-like particles (VLPs) have shown efficacy in nonhuman primates. Previous studies have shown protection of cynomolgus macaques against homologous infection for Ebola virus (EBOV) and Marburg virus (MARV) following a three-dose vaccine regimen of EBOV or MARV VLPs, as well as heterologous protection against Ravn Virus (RAVV) following vaccination with MARV VLPs. The objectives of the current studies were to determine the minimum number of vaccine doses required for protection (using EBOV as the test system) and then demonstrate protection against Sudan virus (SUDV) and Taï Forest virus (TAFV). Using the EBOV nonhuman primate model, we show that one or two doses of VLP vaccine can confer protection from lethal infection. VLPs containing the SUDV glycoprotein, nucleoprotein and VP40 matrix protein provide complete protection against lethal SUDV infection in macaques. Finally, we demonstrate protective efficacy mediated by EBOV, but not SUDV, VLPs against TAFV; this is the first demonstration of complete cross-filovirus protection using a single component heterologous vaccine within the Ebolavirus genus. Along with our previous results, this observation provides strong evidence that it will be possible to develop and administer a broad-spectrum VLP-based vaccine that will protect against multiple filoviruses by combining only three EBOV, SUDV and MARV components. |
| first_indexed | 2024-12-19T02:49:04Z |
| format | Article |
| id | doaj.art-4be6a58712434a6db06a646cdaa6bcab |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-19T02:49:04Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4be6a58712434a6db06a646cdaa6bcab2022-12-21T20:38:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01103e011888110.1371/journal.pone.0118881Homologous and heterologous protection of nonhuman primates by Ebola and Sudan virus-like particles.Kelly L WarfieldJohn M DyeJay B WellsRobert C UnferFrederick W HoltsbergSergey ShuleninHong VuDana L SwensonSina BavariM Javad AmanFiloviruses cause hemorrhagic fever resulting in significant morbidity and mortality in humans. Several vaccine platforms that include multiple virus-vectored approaches and virus-like particles (VLPs) have shown efficacy in nonhuman primates. Previous studies have shown protection of cynomolgus macaques against homologous infection for Ebola virus (EBOV) and Marburg virus (MARV) following a three-dose vaccine regimen of EBOV or MARV VLPs, as well as heterologous protection against Ravn Virus (RAVV) following vaccination with MARV VLPs. The objectives of the current studies were to determine the minimum number of vaccine doses required for protection (using EBOV as the test system) and then demonstrate protection against Sudan virus (SUDV) and Taï Forest virus (TAFV). Using the EBOV nonhuman primate model, we show that one or two doses of VLP vaccine can confer protection from lethal infection. VLPs containing the SUDV glycoprotein, nucleoprotein and VP40 matrix protein provide complete protection against lethal SUDV infection in macaques. Finally, we demonstrate protective efficacy mediated by EBOV, but not SUDV, VLPs against TAFV; this is the first demonstration of complete cross-filovirus protection using a single component heterologous vaccine within the Ebolavirus genus. Along with our previous results, this observation provides strong evidence that it will be possible to develop and administer a broad-spectrum VLP-based vaccine that will protect against multiple filoviruses by combining only three EBOV, SUDV and MARV components.https://doi.org/10.1371/journal.pone.0118881 |
| spellingShingle | Kelly L Warfield John M Dye Jay B Wells Robert C Unfer Frederick W Holtsberg Sergey Shulenin Hong Vu Dana L Swenson Sina Bavari M Javad Aman Homologous and heterologous protection of nonhuman primates by Ebola and Sudan virus-like particles. PLoS ONE |
| title | Homologous and heterologous protection of nonhuman primates by Ebola and Sudan virus-like particles. |
| title_full | Homologous and heterologous protection of nonhuman primates by Ebola and Sudan virus-like particles. |
| title_fullStr | Homologous and heterologous protection of nonhuman primates by Ebola and Sudan virus-like particles. |
| title_full_unstemmed | Homologous and heterologous protection of nonhuman primates by Ebola and Sudan virus-like particles. |
| title_short | Homologous and heterologous protection of nonhuman primates by Ebola and Sudan virus-like particles. |
| title_sort | homologous and heterologous protection of nonhuman primates by ebola and sudan virus like particles |
| url | https://doi.org/10.1371/journal.pone.0118881 |
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