Swim Bladder of Farmed <i>Totoaba macdonaldi</i>: A Source of Value-Added Collagen
Finding strategies to use the swim bladder of farmed totoaba (<i>Totoaba macdonaldi</i>) is of the utmost need to reduce waste. Fish swim bladders are rich in collagen; hence, extracting collagen is a promising alternative with benefits for aquaculture of totoaba and the environment. The...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/3/173 |
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| author | Honorio Cruz-López Sergio Rodríguez-Morales Luis M. Enríquez-Paredes Luis Jesús Villarreal-Gómez Conal True Leticia Olivera-Castillo D. Alejandro Fernández-Velasco Lus M. López |
| author_facet | Honorio Cruz-López Sergio Rodríguez-Morales Luis M. Enríquez-Paredes Luis Jesús Villarreal-Gómez Conal True Leticia Olivera-Castillo D. Alejandro Fernández-Velasco Lus M. López |
| author_sort | Honorio Cruz-López |
| collection | DOAJ |
| description | Finding strategies to use the swim bladder of farmed totoaba (<i>Totoaba macdonaldi</i>) is of the utmost need to reduce waste. Fish swim bladders are rich in collagen; hence, extracting collagen is a promising alternative with benefits for aquaculture of totoaba and the environment. The elemental biochemical composition of totoaba swim bladders, including their proximate and amino acid compositions, was determined. Pepsin-soluble collagen (PSC) was used to extract collagen from swim bladders, and its characteristics were analyzed. Alcalase and papain were used for the preparation of collagen hydrolysates. Swim bladders contained 95% protein, 2.4% fat, and 0.8% ash (on a dry basis). The essential amino acid content was low, but the functional amino acid content was high. The PSC yield was high, at 68% (dry weight). The amino acid composition profile, electrophoretic pattern, and structural integrity analyses of the isolated collagen suggested it is a typical type-I collagen with high purity. The denaturalization temperature was 32.5 °C, probably attributable to the imino acid content (205 residues/1000 residues). Papain-hydrolysates (≤3 kDa) of this collagen exhibited higher radical scavenging activity than Alcalase-hydrolysates. The swim bladder from the farmed totoaba could be an ideal source to produce high-quality type I collagen and may be considered an alternative to conventional collagen sources or bioactive peptides. |
| first_indexed | 2024-03-11T06:16:08Z |
| format | Article |
| id | doaj.art-4be89b4b2d334bacbd6557e6dadb9a6a |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-11T06:16:08Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-4be89b4b2d334bacbd6557e6dadb9a6a2023-11-17T12:17:34ZengMDPI AGMarine Drugs1660-33972023-03-0121317310.3390/md21030173Swim Bladder of Farmed <i>Totoaba macdonaldi</i>: A Source of Value-Added CollagenHonorio Cruz-López0Sergio Rodríguez-Morales1Luis M. Enríquez-Paredes2Luis Jesús Villarreal-Gómez3Conal True4Leticia Olivera-Castillo5D. Alejandro Fernández-Velasco6Lus M. López7Facultad de Ciencias Marinas, Universidad Autónoma de Baja California (UABC), Carretera Transpeninsular Ensenada—Tijuana No. 3917, Col. Playitas, Ensenada 22860, MexicoUnidad de Química en Sisal, Facultad de Química, Universidad Nacional Autónoma de México, Puerto de Abrigo S/N, Sisal, Hunucma 97356, MexicoFacultad de Ciencias Marinas, Universidad Autónoma de Baja California (UABC), Carretera Transpeninsular Ensenada—Tijuana No. 3917, Col. Playitas, Ensenada 22860, MexicoFacultad de Ciencias de la Ingeniería y Tecnología, Universidad Autónoma de Baja California (UABC), Blvd. Universitario 1000, Unidad Valle de las Palmas, Tijuana 22260, MexicoFacultad de Ciencias Marinas, Universidad Autónoma de Baja California (UABC), Carretera Transpeninsular Ensenada—Tijuana No. 3917, Col. Playitas, Ensenada 22860, MexicoCentro de Investigación y de Estudio Avanzados del Instituto Politécnico Nacional—Unidad Mérida, Antigua Carretera a Progreso Km. 6, Merida 97310, MexicoLaboratorio de Fisicoquímica e Ingeniería de Proteínas, Departamento de Bioquímica, Facultad de Medicina, Universidad Nacional Autónoma de México, Ciudad de Mexico 04510, MexicoFacultad de Ciencias Marinas, Universidad Autónoma de Baja California (UABC), Carretera Transpeninsular Ensenada—Tijuana No. 3917, Col. Playitas, Ensenada 22860, MexicoFinding strategies to use the swim bladder of farmed totoaba (<i>Totoaba macdonaldi</i>) is of the utmost need to reduce waste. Fish swim bladders are rich in collagen; hence, extracting collagen is a promising alternative with benefits for aquaculture of totoaba and the environment. The elemental biochemical composition of totoaba swim bladders, including their proximate and amino acid compositions, was determined. Pepsin-soluble collagen (PSC) was used to extract collagen from swim bladders, and its characteristics were analyzed. Alcalase and papain were used for the preparation of collagen hydrolysates. Swim bladders contained 95% protein, 2.4% fat, and 0.8% ash (on a dry basis). The essential amino acid content was low, but the functional amino acid content was high. The PSC yield was high, at 68% (dry weight). The amino acid composition profile, electrophoretic pattern, and structural integrity analyses of the isolated collagen suggested it is a typical type-I collagen with high purity. The denaturalization temperature was 32.5 °C, probably attributable to the imino acid content (205 residues/1000 residues). Papain-hydrolysates (≤3 kDa) of this collagen exhibited higher radical scavenging activity than Alcalase-hydrolysates. The swim bladder from the farmed totoaba could be an ideal source to produce high-quality type I collagen and may be considered an alternative to conventional collagen sources or bioactive peptides.https://www.mdpi.com/1660-3397/21/3/173<i>Totoaba macdonaldi</i>swim bladdercollagen recovery |
| spellingShingle | Honorio Cruz-López Sergio Rodríguez-Morales Luis M. Enríquez-Paredes Luis Jesús Villarreal-Gómez Conal True Leticia Olivera-Castillo D. Alejandro Fernández-Velasco Lus M. López Swim Bladder of Farmed <i>Totoaba macdonaldi</i>: A Source of Value-Added Collagen Marine Drugs <i>Totoaba macdonaldi</i> swim bladder collagen recovery |
| title | Swim Bladder of Farmed <i>Totoaba macdonaldi</i>: A Source of Value-Added Collagen |
| title_full | Swim Bladder of Farmed <i>Totoaba macdonaldi</i>: A Source of Value-Added Collagen |
| title_fullStr | Swim Bladder of Farmed <i>Totoaba macdonaldi</i>: A Source of Value-Added Collagen |
| title_full_unstemmed | Swim Bladder of Farmed <i>Totoaba macdonaldi</i>: A Source of Value-Added Collagen |
| title_short | Swim Bladder of Farmed <i>Totoaba macdonaldi</i>: A Source of Value-Added Collagen |
| title_sort | swim bladder of farmed i totoaba macdonaldi i a source of value added collagen |
| topic | <i>Totoaba macdonaldi</i> swim bladder collagen recovery |
| url | https://www.mdpi.com/1660-3397/21/3/173 |
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