Comparative Proteomics Analysis of Exosomes Identifies Key Pathways and Protein Markers Related to Breast Cancer Metastasis
Proteomics analysis of circulating exosomes derived from cancer cells represents a promising approach to the elucidation of cell–cell communication and the discovery of putative biomarker candidates for cancer diagnosis and treatment. Nonetheless, the proteome of exosomes derived from cell lines wit...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-02-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/4/4033 |
| _version_ | 1797620396522995712 |
|---|---|
| author | Shichen Shen Chengjian Tu He Shen Jun Li Costa Frangou Jianmin Zhang Jun Qu |
| author_facet | Shichen Shen Chengjian Tu He Shen Jun Li Costa Frangou Jianmin Zhang Jun Qu |
| author_sort | Shichen Shen |
| collection | DOAJ |
| description | Proteomics analysis of circulating exosomes derived from cancer cells represents a promising approach to the elucidation of cell–cell communication and the discovery of putative biomarker candidates for cancer diagnosis and treatment. Nonetheless, the proteome of exosomes derived from cell lines with different metastatic capabilities still warrants further investigation. Here, we present a comprehensive quantitative proteomics investigation of exosomes isolated from immortalized mammary epithelial cells and matched tumor lines with different metastatic potentials in an attempt to discover exosome markers specific to breast cancer (BC) metastasis. A total of 2135 unique proteins were quantified with a high confidence level from 20 isolated exosome samples, including 94 of the TOP 100 exosome markers archived by ExoCarta. Moreover, 348 altered proteins were observed, among which several metastasis-specific markers, including cathepsin W (CATW), magnesium transporter MRS2 (MRS2), syntenin-2 (SDCB2), reticulon-4 (RTN), and UV excision repair protein RAD23 homolog (RAD23B), were also identified. Notably, the abundance of these metastasis-specific markers corresponds well with the overall survival of BC patients in clinical settings. Together, these data provide a valuable dataset for BC exosome proteomics investigation and prominently facilitate the elucidation of the molecular mechanisms underlying primary tumor development and progression. |
| first_indexed | 2024-03-11T08:40:45Z |
| format | Article |
| id | doaj.art-4beaffe190be4d1eb1ebe3e0d7e60879 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T08:40:45Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4beaffe190be4d1eb1ebe3e0d7e608792023-11-16T21:08:31ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-02-01244403310.3390/ijms24044033Comparative Proteomics Analysis of Exosomes Identifies Key Pathways and Protein Markers Related to Breast Cancer MetastasisShichen Shen0Chengjian Tu1He Shen2Jun Li3Costa Frangou4Jianmin Zhang5Jun Qu6Department of Pharmaceutical Sciences, University at Buffalo, Buffalo, NY 14214, USADepartment of Pharmaceutical Sciences, University at Buffalo, Buffalo, NY 14214, USADepartment of Cancer Genetics and Genomics, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USADepartment of Pharmaceutical Sciences, University at Buffalo, Buffalo, NY 14214, USADepartment of Molecular and Cellular Biology, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USADepartment of Cancer Genetics and Genomics, Roswell Park Comprehensive Cancer Center, Buffalo, NY 14263, USADepartment of Pharmaceutical Sciences, University at Buffalo, Buffalo, NY 14214, USAProteomics analysis of circulating exosomes derived from cancer cells represents a promising approach to the elucidation of cell–cell communication and the discovery of putative biomarker candidates for cancer diagnosis and treatment. Nonetheless, the proteome of exosomes derived from cell lines with different metastatic capabilities still warrants further investigation. Here, we present a comprehensive quantitative proteomics investigation of exosomes isolated from immortalized mammary epithelial cells and matched tumor lines with different metastatic potentials in an attempt to discover exosome markers specific to breast cancer (BC) metastasis. A total of 2135 unique proteins were quantified with a high confidence level from 20 isolated exosome samples, including 94 of the TOP 100 exosome markers archived by ExoCarta. Moreover, 348 altered proteins were observed, among which several metastasis-specific markers, including cathepsin W (CATW), magnesium transporter MRS2 (MRS2), syntenin-2 (SDCB2), reticulon-4 (RTN), and UV excision repair protein RAD23 homolog (RAD23B), were also identified. Notably, the abundance of these metastasis-specific markers corresponds well with the overall survival of BC patients in clinical settings. Together, these data provide a valuable dataset for BC exosome proteomics investigation and prominently facilitate the elucidation of the molecular mechanisms underlying primary tumor development and progression.https://www.mdpi.com/1422-0067/24/4/4033exosomesbreast cancermetastasisquantitative proteomicslabel-free quantificationIonStar |
| spellingShingle | Shichen Shen Chengjian Tu He Shen Jun Li Costa Frangou Jianmin Zhang Jun Qu Comparative Proteomics Analysis of Exosomes Identifies Key Pathways and Protein Markers Related to Breast Cancer Metastasis International Journal of Molecular Sciences exosomes breast cancer metastasis quantitative proteomics label-free quantification IonStar |
| title | Comparative Proteomics Analysis of Exosomes Identifies Key Pathways and Protein Markers Related to Breast Cancer Metastasis |
| title_full | Comparative Proteomics Analysis of Exosomes Identifies Key Pathways and Protein Markers Related to Breast Cancer Metastasis |
| title_fullStr | Comparative Proteomics Analysis of Exosomes Identifies Key Pathways and Protein Markers Related to Breast Cancer Metastasis |
| title_full_unstemmed | Comparative Proteomics Analysis of Exosomes Identifies Key Pathways and Protein Markers Related to Breast Cancer Metastasis |
| title_short | Comparative Proteomics Analysis of Exosomes Identifies Key Pathways and Protein Markers Related to Breast Cancer Metastasis |
| title_sort | comparative proteomics analysis of exosomes identifies key pathways and protein markers related to breast cancer metastasis |
| topic | exosomes breast cancer metastasis quantitative proteomics label-free quantification IonStar |
| url | https://www.mdpi.com/1422-0067/24/4/4033 |
| work_keys_str_mv | AT shichenshen comparativeproteomicsanalysisofexosomesidentifieskeypathwaysandproteinmarkersrelatedtobreastcancermetastasis AT chengjiantu comparativeproteomicsanalysisofexosomesidentifieskeypathwaysandproteinmarkersrelatedtobreastcancermetastasis AT heshen comparativeproteomicsanalysisofexosomesidentifieskeypathwaysandproteinmarkersrelatedtobreastcancermetastasis AT junli comparativeproteomicsanalysisofexosomesidentifieskeypathwaysandproteinmarkersrelatedtobreastcancermetastasis AT costafrangou comparativeproteomicsanalysisofexosomesidentifieskeypathwaysandproteinmarkersrelatedtobreastcancermetastasis AT jianminzhang comparativeproteomicsanalysisofexosomesidentifieskeypathwaysandproteinmarkersrelatedtobreastcancermetastasis AT junqu comparativeproteomicsanalysisofexosomesidentifieskeypathwaysandproteinmarkersrelatedtobreastcancermetastasis |