Phytochemical Profile, Antioxidant Capacity, α-Amylase and α-Glucosidase Inhibitory Potential of Wild Moroccan <i>Inula viscosa</i> (L.) <i>Aiton</i> Leaves
Medicinal plants offer imperative sources of innovative chemical substances with important potential therapeutic effects. Among them, the members of the genus <i>Inula</i> have been widely used in traditional medicine for the treatment of several diseases. The present study investigated...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-05-01
|
| Series: | Molecules |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1420-3049/26/11/3134 |
| _version_ | 1797532854454845440 |
|---|---|
| author | Fadoua Asraoui Ayoub Kounnoun Francesco Cacciola Fouad El Mansouri Imad Kabach Yassine Oulad El Majdoub Filippo Alibrando Katia Arena Emanuela Trovato Luigi Mondello Adnane Louajri |
| author_facet | Fadoua Asraoui Ayoub Kounnoun Francesco Cacciola Fouad El Mansouri Imad Kabach Yassine Oulad El Majdoub Filippo Alibrando Katia Arena Emanuela Trovato Luigi Mondello Adnane Louajri |
| author_sort | Fadoua Asraoui |
| collection | DOAJ |
| description | Medicinal plants offer imperative sources of innovative chemical substances with important potential therapeutic effects. Among them, the members of the genus <i>Inula</i> have been widely used in traditional medicine for the treatment of several diseases. The present study investigated the antioxidant (DPPH, ABTS and FRAP assays) and the in vitro anti-hyperglycemic potential of aerial parts of <i>Inula viscosa</i> (L.) <i>Aiton</i> (<i>I. viscosa</i>) extracts through the inhibition of digestive enzymes (α-amylase and α-glucosidase), responsible of the digestion of poly and oligosaccharides. The polyphenolic profile of the <i>Inula viscosa</i> (L.) <i>Aiton</i> EtOAc extract was also investigated using HPLC-DAD/ESI-MS analysis, whereas the volatile composition was elucidated by GC-MS. The chemical analysis resulted in the detection of twenty-one polyphenolic compounds, whereas the volatile profile highlighted the occurrence of forty-eight different compounds. <i>Inula viscosa</i> (L.) <i>Aiton</i> presented values as high as 87.2 ± 0.50 mg GAE/g and 78.6 ± 0.55mg CE/g, for gallic acid and catechin, respectively. The EtOAc extract exhibited the higher antioxidant activity compared to methanol and chloroform extracts in different tests with (IC<sub>50</sub> = 0.6 ± 0.03 µg/mL; IC<sub>50</sub> = 8.6 ± 0.08 µg/mL; 634.8 mg ± 1.45 AAE/g extract) in DPPH, ABTS and FRAP tests. Moreover, <i>Inula viscosa</i> (L.) <i>Aiton</i> leaves did show an important inhibitory effect against α-amylase and α-glucosidase. On the basis of the results achieved, such a species represents a promising traditional medicine, thanks to its remarkable content of functional bioactive compounds, thus opening new prospects for research and innovative phytopharmaceuticals developments. |
| first_indexed | 2024-03-10T11:06:16Z |
| format | Article |
| id | doaj.art-4bf00ce831954f8bb8231a932cc3260e |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T11:06:16Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4bf00ce831954f8bb8231a932cc3260e2023-11-21T21:08:44ZengMDPI AGMolecules1420-30492021-05-012611313410.3390/molecules26113134Phytochemical Profile, Antioxidant Capacity, α-Amylase and α-Glucosidase Inhibitory Potential of Wild Moroccan <i>Inula viscosa</i> (L.) <i>Aiton</i> LeavesFadoua Asraoui0Ayoub Kounnoun1Francesco Cacciola2Fouad El Mansouri3Imad Kabach4Yassine Oulad El Majdoub5Filippo Alibrando6Katia Arena7Emanuela Trovato8Luigi Mondello9Adnane Louajri10Laboratory of Applied Biology and Pathology, Department of Biology, Faculty of Sciences of Tetouan, Abdelmalek Essaâdi University, Tetouan 93000, MoroccoLaboratory of Applied Biology and Pathology, Department of Biology, Faculty of Sciences of Tetouan, Abdelmalek Essaâdi University, Tetouan 93000, MoroccoDepartment of Biomedical, Dental, Morphological and Functional Imaging Sciences, University of Messina, 98125 Messina, ItalyLaboratory of Chemical Engineering and Valorization of Resources, Department of Chemistry, Faculty of Sciences and Technology, Abdelmalek Essaâdi University, Tangier 416, MoroccoLaboratory of Biochemistry and Molecular Genetics, Faculty of Sciences and Technologies of Tangier, Tangier 416, MoroccoDepartment of Chemical Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, ItalyChromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, ItalyDepartment of Chemical Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, ItalyChromaleont s.r.l., c/o Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, ItalyDepartment of Chemical Biological, Pharmaceutical and Environmental Sciences, University of Messina, 98168 Messina, ItalyLaboratory of Applied Biology and Pathology, Department of Biology, Faculty of Sciences of Tetouan, Abdelmalek Essaâdi University, Tetouan 93000, MoroccoMedicinal plants offer imperative sources of innovative chemical substances with important potential therapeutic effects. Among them, the members of the genus <i>Inula</i> have been widely used in traditional medicine for the treatment of several diseases. The present study investigated the antioxidant (DPPH, ABTS and FRAP assays) and the in vitro anti-hyperglycemic potential of aerial parts of <i>Inula viscosa</i> (L.) <i>Aiton</i> (<i>I. viscosa</i>) extracts through the inhibition of digestive enzymes (α-amylase and α-glucosidase), responsible of the digestion of poly and oligosaccharides. The polyphenolic profile of the <i>Inula viscosa</i> (L.) <i>Aiton</i> EtOAc extract was also investigated using HPLC-DAD/ESI-MS analysis, whereas the volatile composition was elucidated by GC-MS. The chemical analysis resulted in the detection of twenty-one polyphenolic compounds, whereas the volatile profile highlighted the occurrence of forty-eight different compounds. <i>Inula viscosa</i> (L.) <i>Aiton</i> presented values as high as 87.2 ± 0.50 mg GAE/g and 78.6 ± 0.55mg CE/g, for gallic acid and catechin, respectively. The EtOAc extract exhibited the higher antioxidant activity compared to methanol and chloroform extracts in different tests with (IC<sub>50</sub> = 0.6 ± 0.03 µg/mL; IC<sub>50</sub> = 8.6 ± 0.08 µg/mL; 634.8 mg ± 1.45 AAE/g extract) in DPPH, ABTS and FRAP tests. Moreover, <i>Inula viscosa</i> (L.) <i>Aiton</i> leaves did show an important inhibitory effect against α-amylase and α-glucosidase. On the basis of the results achieved, such a species represents a promising traditional medicine, thanks to its remarkable content of functional bioactive compounds, thus opening new prospects for research and innovative phytopharmaceuticals developments.https://www.mdpi.com/1420-3049/26/11/3134<i>Inula viscosa</i> (L.) <i>Aiton</i>polyphenolic compoundsflavonoidsHPLC-DAD/ESI-MSGC-MSα-amylase |
| spellingShingle | Fadoua Asraoui Ayoub Kounnoun Francesco Cacciola Fouad El Mansouri Imad Kabach Yassine Oulad El Majdoub Filippo Alibrando Katia Arena Emanuela Trovato Luigi Mondello Adnane Louajri Phytochemical Profile, Antioxidant Capacity, α-Amylase and α-Glucosidase Inhibitory Potential of Wild Moroccan <i>Inula viscosa</i> (L.) <i>Aiton</i> Leaves Molecules <i>Inula viscosa</i> (L.) <i>Aiton</i> polyphenolic compounds flavonoids HPLC-DAD/ESI-MS GC-MS α-amylase |
| title | Phytochemical Profile, Antioxidant Capacity, α-Amylase and α-Glucosidase Inhibitory Potential of Wild Moroccan <i>Inula viscosa</i> (L.) <i>Aiton</i> Leaves |
| title_full | Phytochemical Profile, Antioxidant Capacity, α-Amylase and α-Glucosidase Inhibitory Potential of Wild Moroccan <i>Inula viscosa</i> (L.) <i>Aiton</i> Leaves |
| title_fullStr | Phytochemical Profile, Antioxidant Capacity, α-Amylase and α-Glucosidase Inhibitory Potential of Wild Moroccan <i>Inula viscosa</i> (L.) <i>Aiton</i> Leaves |
| title_full_unstemmed | Phytochemical Profile, Antioxidant Capacity, α-Amylase and α-Glucosidase Inhibitory Potential of Wild Moroccan <i>Inula viscosa</i> (L.) <i>Aiton</i> Leaves |
| title_short | Phytochemical Profile, Antioxidant Capacity, α-Amylase and α-Glucosidase Inhibitory Potential of Wild Moroccan <i>Inula viscosa</i> (L.) <i>Aiton</i> Leaves |
| title_sort | phytochemical profile antioxidant capacity α amylase and α glucosidase inhibitory potential of wild moroccan i inula viscosa i l i aiton i leaves |
| topic | <i>Inula viscosa</i> (L.) <i>Aiton</i> polyphenolic compounds flavonoids HPLC-DAD/ESI-MS GC-MS α-amylase |
| url | https://www.mdpi.com/1420-3049/26/11/3134 |
| work_keys_str_mv | AT fadouaasraoui phytochemicalprofileantioxidantcapacityaamylaseandaglucosidaseinhibitorypotentialofwildmoroccaniinulaviscosailiaitonileaves AT ayoubkounnoun phytochemicalprofileantioxidantcapacityaamylaseandaglucosidaseinhibitorypotentialofwildmoroccaniinulaviscosailiaitonileaves AT francescocacciola phytochemicalprofileantioxidantcapacityaamylaseandaglucosidaseinhibitorypotentialofwildmoroccaniinulaviscosailiaitonileaves AT fouadelmansouri phytochemicalprofileantioxidantcapacityaamylaseandaglucosidaseinhibitorypotentialofwildmoroccaniinulaviscosailiaitonileaves AT imadkabach phytochemicalprofileantioxidantcapacityaamylaseandaglucosidaseinhibitorypotentialofwildmoroccaniinulaviscosailiaitonileaves AT yassineouladelmajdoub phytochemicalprofileantioxidantcapacityaamylaseandaglucosidaseinhibitorypotentialofwildmoroccaniinulaviscosailiaitonileaves AT filippoalibrando phytochemicalprofileantioxidantcapacityaamylaseandaglucosidaseinhibitorypotentialofwildmoroccaniinulaviscosailiaitonileaves AT katiaarena phytochemicalprofileantioxidantcapacityaamylaseandaglucosidaseinhibitorypotentialofwildmoroccaniinulaviscosailiaitonileaves AT emanuelatrovato phytochemicalprofileantioxidantcapacityaamylaseandaglucosidaseinhibitorypotentialofwildmoroccaniinulaviscosailiaitonileaves AT luigimondello phytochemicalprofileantioxidantcapacityaamylaseandaglucosidaseinhibitorypotentialofwildmoroccaniinulaviscosailiaitonileaves AT adnanelouajri phytochemicalprofileantioxidantcapacityaamylaseandaglucosidaseinhibitorypotentialofwildmoroccaniinulaviscosailiaitonileaves |