Mpp23Aa/Xpp37Aa Insecticidal Proteins from <i>Bacillus thuringiensis</i> (Bacillales: Bacillaceae) Are Highly Toxic to <i>Anthonomus grandis</i> (Coleoptera: Curculionidae) Larvae
The beetle <i>Anthonomus grandis</i> Boheman, 1843, is the main cotton pest, causing enormous losses in cotton. The breeding of genetically modified plants with <i>A. grandis</i> resistance is seen as an important control strategy. However, the identification of molecules wit...
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MDPI AG
2023-01-01
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| Series: | Toxins |
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| author | Jéssica A. de Oliveira Bárbara F. Negri Patricia Hernández-Martínez Marcos F. Basso Baltasar Escriche |
| author_facet | Jéssica A. de Oliveira Bárbara F. Negri Patricia Hernández-Martínez Marcos F. Basso Baltasar Escriche |
| author_sort | Jéssica A. de Oliveira |
| collection | DOAJ |
| description | The beetle <i>Anthonomus grandis</i> Boheman, 1843, is the main cotton pest, causing enormous losses in cotton. The breeding of genetically modified plants with <i>A. grandis</i> resistance is seen as an important control strategy. However, the identification of molecules with high toxicity to this insect remains a challenge. The susceptibility of <i>A. grandis</i> larvae to proteins (Cry1Ba, Cry7Ab, and Mpp23Aa/Xpp37Aa) from <i>Bacillus thuringiensis</i> Berliner, 1915, with toxicity reported against Coleopteran, has been evaluated. The ingestion of different protein concentrations (which were incorporated into an artificial diet) by the larvae was tested in the laboratory, and mortality was evaluated after one week. All Cry proteins tested exhibited higher toxicity than that the untreated artificial diet. These Cry proteins showed similar results to the control Cry1Ac, with low toxicity to <i>A. grandis,</i> since it killed less than 50% of larvae, even at the highest concentration applied (100 μg·g<sup>−1</sup>). Mpp/Xpp proteins provided the highest toxicity with a 0.18 μg·g<sup>−1</sup> value for the 50% lethal concentration. Importantly, this parameter is the lowest ever reported for this insect species tested with <i>B. thuringiensis</i> proteins. This result highlights the potential of Mpp23Aa/Xpp37Aa for the development of a biotechnological tool aiming at the field control of <i>A. grandis</i>. |
| first_indexed | 2024-03-09T11:06:01Z |
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| last_indexed | 2024-03-09T11:06:01Z |
| publishDate | 2023-01-01 |
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| series | Toxins |
| spelling | doaj.art-4bf0d25d1808436fa8f9d67151a1292f2023-12-01T00:57:12ZengMDPI AGToxins2072-66512023-01-011515510.3390/toxins15010055Mpp23Aa/Xpp37Aa Insecticidal Proteins from <i>Bacillus thuringiensis</i> (Bacillales: Bacillaceae) Are Highly Toxic to <i>Anthonomus grandis</i> (Coleoptera: Curculionidae) LarvaeJéssica A. de Oliveira0Bárbara F. Negri1Patricia Hernández-Martínez2Marcos F. Basso3Baltasar Escriche4Laboratório de Prospecção de Cepas e Genes, Instituto Mato-Grossense do Algodão (IMAmt), Rondonópolis 78740-970, Mato Grosso, BrazilLaboratório de Biologia Molecular e Transformação de Plantas, Instituto Mato-Grossense do Algodão (IMAmt), Rondonópolis 78740-970, Mato Grosso, BrazilDepartamento de Genética, Instituto de Biotecnología y Biomedicina (BIOTECMED), Universitat de València, 46100 Burjassot, Valencia, SpainDipartimento di Biologia e Incubatore Universitario Fiorentino, Dipartimento di Biologia, Università degli Studi di Firenze, 50019 Sesto Fiorentino, Firenze, ItalyDepartamento de Genética, Instituto de Biotecnología y Biomedicina (BIOTECMED), Universitat de València, 46100 Burjassot, Valencia, SpainThe beetle <i>Anthonomus grandis</i> Boheman, 1843, is the main cotton pest, causing enormous losses in cotton. The breeding of genetically modified plants with <i>A. grandis</i> resistance is seen as an important control strategy. However, the identification of molecules with high toxicity to this insect remains a challenge. The susceptibility of <i>A. grandis</i> larvae to proteins (Cry1Ba, Cry7Ab, and Mpp23Aa/Xpp37Aa) from <i>Bacillus thuringiensis</i> Berliner, 1915, with toxicity reported against Coleopteran, has been evaluated. The ingestion of different protein concentrations (which were incorporated into an artificial diet) by the larvae was tested in the laboratory, and mortality was evaluated after one week. All Cry proteins tested exhibited higher toxicity than that the untreated artificial diet. These Cry proteins showed similar results to the control Cry1Ac, with low toxicity to <i>A. grandis,</i> since it killed less than 50% of larvae, even at the highest concentration applied (100 μg·g<sup>−1</sup>). Mpp/Xpp proteins provided the highest toxicity with a 0.18 μg·g<sup>−1</sup> value for the 50% lethal concentration. Importantly, this parameter is the lowest ever reported for this insect species tested with <i>B. thuringiensis</i> proteins. This result highlights the potential of Mpp23Aa/Xpp37Aa for the development of a biotechnological tool aiming at the field control of <i>A. grandis</i>.https://www.mdpi.com/2072-6651/15/1/55cotton boll weevilCry proteinsMpp/Xpp proteinsCry23AaCry37AaColeoptera |
| spellingShingle | Jéssica A. de Oliveira Bárbara F. Negri Patricia Hernández-Martínez Marcos F. Basso Baltasar Escriche Mpp23Aa/Xpp37Aa Insecticidal Proteins from <i>Bacillus thuringiensis</i> (Bacillales: Bacillaceae) Are Highly Toxic to <i>Anthonomus grandis</i> (Coleoptera: Curculionidae) Larvae Toxins cotton boll weevil Cry proteins Mpp/Xpp proteins Cry23Aa Cry37Aa Coleoptera |
| title | Mpp23Aa/Xpp37Aa Insecticidal Proteins from <i>Bacillus thuringiensis</i> (Bacillales: Bacillaceae) Are Highly Toxic to <i>Anthonomus grandis</i> (Coleoptera: Curculionidae) Larvae |
| title_full | Mpp23Aa/Xpp37Aa Insecticidal Proteins from <i>Bacillus thuringiensis</i> (Bacillales: Bacillaceae) Are Highly Toxic to <i>Anthonomus grandis</i> (Coleoptera: Curculionidae) Larvae |
| title_fullStr | Mpp23Aa/Xpp37Aa Insecticidal Proteins from <i>Bacillus thuringiensis</i> (Bacillales: Bacillaceae) Are Highly Toxic to <i>Anthonomus grandis</i> (Coleoptera: Curculionidae) Larvae |
| title_full_unstemmed | Mpp23Aa/Xpp37Aa Insecticidal Proteins from <i>Bacillus thuringiensis</i> (Bacillales: Bacillaceae) Are Highly Toxic to <i>Anthonomus grandis</i> (Coleoptera: Curculionidae) Larvae |
| title_short | Mpp23Aa/Xpp37Aa Insecticidal Proteins from <i>Bacillus thuringiensis</i> (Bacillales: Bacillaceae) Are Highly Toxic to <i>Anthonomus grandis</i> (Coleoptera: Curculionidae) Larvae |
| title_sort | mpp23aa xpp37aa insecticidal proteins from i bacillus thuringiensis i bacillales bacillaceae are highly toxic to i anthonomus grandis i coleoptera curculionidae larvae |
| topic | cotton boll weevil Cry proteins Mpp/Xpp proteins Cry23Aa Cry37Aa Coleoptera |
| url | https://www.mdpi.com/2072-6651/15/1/55 |
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