In vitro adhesion and invasion inhibition of <it>Shigella dysenteriae</it>, <it>Shigella flexneri </it>and <it>Shigella sonnei </it>clinical strains by human milk proteins
<p>Abstract</p> <p>Background</p> <p><it>Shigella </it>is the etiological agent of shigellosis, a disease responsible for more than 500,000 deaths of children per year, in developing countries. These pathogens colonize the intestinal colon, invade, spreading...
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| Format: | Article |
| Language: | English |
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BMC
2004-04-01
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| Series: | BMC Microbiology |
| Online Access: | http://www.biomedcentral.com/1471-2180/4/18 |
| _version_ | 1828486765638320128 |
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| author | Giugliano Loreny Lima Renato de Willer Emerson |
| author_facet | Giugliano Loreny Lima Renato de Willer Emerson |
| author_sort | Giugliano Loreny |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p><it>Shigella </it>is the etiological agent of shigellosis, a disease responsible for more than 500,000 deaths of children per year, in developing countries. These pathogens colonize the intestinal colon, invade, spreading to the other enterocytes. Breastfeeding plays a very important role in protecting infants from intestinal infections. Amongst milk compounds, glycosylated proteins prevent the adhesion of many enteropathogens <it>in vitro</it>. The aim of this work was to determine the effect of human milk proteins on the colonization potential of <it>Shigella dysenteriae, S. flexneri and S. sonnei</it>. To fulfill this purpose, pooled milk samples from five donors, were fractionated by gel filtration and affinity chromatography. Using tissue culture, the milk fractions obtained were tested in <it>Shigella </it>adhesion and invasion assays.</p> <p>Results</p> <p>Our revealed showed that both adhesion and invasion of <it>Shigella </it>species were inhibited by low concentration of secretory immunoglobulin A, lactoferrin and free secretory component. This work also showed that, these proteins bind to superficial and whole-cell <it>Shigella </it>proteins.</p> <p>Conclusions</p> <p>Our findings suggest that human milk may act inhibiting adhesion and, consequently, invasion of <it>Shigella</it>, thereafter preventing shigellosis in infants.</p> |
| first_indexed | 2024-12-11T09:38:55Z |
| format | Article |
| id | doaj.art-4bf11f14889d4fdb883805947d164839 |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-12-11T09:38:55Z |
| publishDate | 2004-04-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-4bf11f14889d4fdb883805947d1648392022-12-22T01:12:44ZengBMCBMC Microbiology1471-21802004-04-01411810.1186/1471-2180-4-18In vitro adhesion and invasion inhibition of <it>Shigella dysenteriae</it>, <it>Shigella flexneri </it>and <it>Shigella sonnei </it>clinical strains by human milk proteinsGiugliano LorenyLima Renato deWiller Emerson<p>Abstract</p> <p>Background</p> <p><it>Shigella </it>is the etiological agent of shigellosis, a disease responsible for more than 500,000 deaths of children per year, in developing countries. These pathogens colonize the intestinal colon, invade, spreading to the other enterocytes. Breastfeeding plays a very important role in protecting infants from intestinal infections. Amongst milk compounds, glycosylated proteins prevent the adhesion of many enteropathogens <it>in vitro</it>. The aim of this work was to determine the effect of human milk proteins on the colonization potential of <it>Shigella dysenteriae, S. flexneri and S. sonnei</it>. To fulfill this purpose, pooled milk samples from five donors, were fractionated by gel filtration and affinity chromatography. Using tissue culture, the milk fractions obtained were tested in <it>Shigella </it>adhesion and invasion assays.</p> <p>Results</p> <p>Our revealed showed that both adhesion and invasion of <it>Shigella </it>species were inhibited by low concentration of secretory immunoglobulin A, lactoferrin and free secretory component. This work also showed that, these proteins bind to superficial and whole-cell <it>Shigella </it>proteins.</p> <p>Conclusions</p> <p>Our findings suggest that human milk may act inhibiting adhesion and, consequently, invasion of <it>Shigella</it>, thereafter preventing shigellosis in infants.</p>http://www.biomedcentral.com/1471-2180/4/18 |
| spellingShingle | Giugliano Loreny Lima Renato de Willer Emerson In vitro adhesion and invasion inhibition of <it>Shigella dysenteriae</it>, <it>Shigella flexneri </it>and <it>Shigella sonnei </it>clinical strains by human milk proteins BMC Microbiology |
| title | In vitro adhesion and invasion inhibition of <it>Shigella dysenteriae</it>, <it>Shigella flexneri </it>and <it>Shigella sonnei </it>clinical strains by human milk proteins |
| title_full | In vitro adhesion and invasion inhibition of <it>Shigella dysenteriae</it>, <it>Shigella flexneri </it>and <it>Shigella sonnei </it>clinical strains by human milk proteins |
| title_fullStr | In vitro adhesion and invasion inhibition of <it>Shigella dysenteriae</it>, <it>Shigella flexneri </it>and <it>Shigella sonnei </it>clinical strains by human milk proteins |
| title_full_unstemmed | In vitro adhesion and invasion inhibition of <it>Shigella dysenteriae</it>, <it>Shigella flexneri </it>and <it>Shigella sonnei </it>clinical strains by human milk proteins |
| title_short | In vitro adhesion and invasion inhibition of <it>Shigella dysenteriae</it>, <it>Shigella flexneri </it>and <it>Shigella sonnei </it>clinical strains by human milk proteins |
| title_sort | in vitro adhesion and invasion inhibition of it shigella dysenteriae it it shigella flexneri it and it shigella sonnei it clinical strains by human milk proteins |
| url | http://www.biomedcentral.com/1471-2180/4/18 |
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