Theoretical—Experimental Approach of Chitosan/Quaternized Chitosan Nanofibers’ Behavior in Wound Exudate Media
This study aimed to investigate the behavior of chitosan/quaternized chitosan fibers in media mimicking wound exudates to understand their capacities as wound dressing. Fiber analysis of the fibers using dynamic vapor sorption proved their ability to adsorb moisture up to 60% and then to desorb it a...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-12-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/15/12/2722 |
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| author | Bianca-Iustina Andreica Alexandru Anisiei Manuela-Maria Iftime Razvan-Vasile Ababei Lacramioara Ochiuz Decebal Vasincu Ingrid-Andrada Vasilache Constantin Volovat Diana Boboc Vladimir Poroch Lucian Eva Maricel Agop Dragos-Viorel Scripcariu Simona Ruxandra Volovat |
| author_facet | Bianca-Iustina Andreica Alexandru Anisiei Manuela-Maria Iftime Razvan-Vasile Ababei Lacramioara Ochiuz Decebal Vasincu Ingrid-Andrada Vasilache Constantin Volovat Diana Boboc Vladimir Poroch Lucian Eva Maricel Agop Dragos-Viorel Scripcariu Simona Ruxandra Volovat |
| author_sort | Bianca-Iustina Andreica |
| collection | DOAJ |
| description | This study aimed to investigate the behavior of chitosan/quaternized chitosan fibers in media mimicking wound exudates to understand their capacities as wound dressing. Fiber analysis of the fibers using dynamic vapor sorption proved their ability to adsorb moisture up to 60% and then to desorb it as a function of humidity, indicating their outstanding breathability. Dissolution analyses showed that quaternized chitosan leached from the fibers in water and PBS, whereas only small portions of chitosan were solubilized in water. In media containing lysozyme, the fibers degraded with a rate determined by their composition and pH, reaching a mass loss of up to 47% in media of physiologic pH. Notably, in media mimicking the wound exudate during healing, they adsorbed moisture even when their mass loss due to biodegradation was high, whereas they were completely degraded in the media of normal tissues, indicating bioabsorbable dressing capacities. A mathematical model was constructed, which characterized the degradation rate and morphology changes of chitosan/quaternized chitosan fibers through analyses of dynamics in scale space, using the Theory of Scale Relativity. The model was validated using experimental data, making it possible to generalize it to the degradation of other biopolymeric systems that address wound healing. |
| first_indexed | 2024-03-08T20:26:51Z |
| format | Article |
| id | doaj.art-4bf4243058be4d92afdf6f5cd9492d38 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-08T20:26:51Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-4bf4243058be4d92afdf6f5cd9492d382023-12-22T14:32:07ZengMDPI AGPharmaceutics1999-49232023-12-011512272210.3390/pharmaceutics15122722Theoretical—Experimental Approach of Chitosan/Quaternized Chitosan Nanofibers’ Behavior in Wound Exudate MediaBianca-Iustina Andreica0Alexandru Anisiei1Manuela-Maria Iftime2Razvan-Vasile Ababei3Lacramioara Ochiuz4Decebal Vasincu5Ingrid-Andrada Vasilache6Constantin Volovat7Diana Boboc8Vladimir Poroch9Lucian Eva10Maricel Agop11Dragos-Viorel Scripcariu12Simona Ruxandra Volovat13“Petru Poni” Institute of Macromolecular Chemistry, Gr. Ghica Voda Alley, 41A, 700487 Iasi, Romania“Petru Poni” Institute of Macromolecular Chemistry, Gr. Ghica Voda Alley, 41A, 700487 Iasi, Romania“Petru Poni” Institute of Macromolecular Chemistry, Gr. Ghica Voda Alley, 41A, 700487 Iasi, RomaniaLaboratory of Applied Meteorology and Climatology, A Building, Physics, Research Center with Integrated Techniques for Atmospheric Aerosol Investigation in Romania, RECENT AIR, Alexandru Ioan Cuza University of Iasi, 11 Carol I, 700506 Iasi, RomaniaFaculty of Pharmacy, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, RomaniaDepartment of Biophysics, Faculty of Dental Medicine, “Grigore T. Popa” University of Medicine and Pharmacy, Iasi 700115, RomaniaDepartment of Obstetrics and Gynecology, “Grigore T. Popa” University of Medicine and Pharmacy, 700115 Iasi, RomaniaDepartment of Medical Oncology-Radiotherapy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Str., 700115 Iasi, RomaniaDepartment of Medical Oncology-Radiotherapy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Str., 700115 Iasi, RomaniaFaculty of Medicine, “Grigore T. Popa” University of Medicine and Pharmacy Iasi, 700115 Iasi, Romania“Prof. Dr. Nicolae Oblu” Emergency Clinical Hospital, 2 Ateneului Street, 700309 Iasi, RomaniaDepartment of Physics, “Gheorghe Asachi” Technical University of Iasi, 700050 Iasi, RomaniaDepartment of Surgery, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Str., 700115 Iasi, RomaniaDepartment of Medical Oncology-Radiotherapy, “Grigore T. Popa” University of Medicine and Pharmacy, 16 University Str., 700115 Iasi, RomaniaThis study aimed to investigate the behavior of chitosan/quaternized chitosan fibers in media mimicking wound exudates to understand their capacities as wound dressing. Fiber analysis of the fibers using dynamic vapor sorption proved their ability to adsorb moisture up to 60% and then to desorb it as a function of humidity, indicating their outstanding breathability. Dissolution analyses showed that quaternized chitosan leached from the fibers in water and PBS, whereas only small portions of chitosan were solubilized in water. In media containing lysozyme, the fibers degraded with a rate determined by their composition and pH, reaching a mass loss of up to 47% in media of physiologic pH. Notably, in media mimicking the wound exudate during healing, they adsorbed moisture even when their mass loss due to biodegradation was high, whereas they were completely degraded in the media of normal tissues, indicating bioabsorbable dressing capacities. A mathematical model was constructed, which characterized the degradation rate and morphology changes of chitosan/quaternized chitosan fibers through analyses of dynamics in scale space, using the Theory of Scale Relativity. The model was validated using experimental data, making it possible to generalize it to the degradation of other biopolymeric systems that address wound healing.https://www.mdpi.com/1999-4923/15/12/2722chitosan fibersbiomaterialsengineering nanomaterialscomposite nanomaterialsenzymatic degradation |
| spellingShingle | Bianca-Iustina Andreica Alexandru Anisiei Manuela-Maria Iftime Razvan-Vasile Ababei Lacramioara Ochiuz Decebal Vasincu Ingrid-Andrada Vasilache Constantin Volovat Diana Boboc Vladimir Poroch Lucian Eva Maricel Agop Dragos-Viorel Scripcariu Simona Ruxandra Volovat Theoretical—Experimental Approach of Chitosan/Quaternized Chitosan Nanofibers’ Behavior in Wound Exudate Media Pharmaceutics chitosan fibers biomaterials engineering nanomaterials composite nanomaterials enzymatic degradation |
| title | Theoretical—Experimental Approach of Chitosan/Quaternized Chitosan Nanofibers’ Behavior in Wound Exudate Media |
| title_full | Theoretical—Experimental Approach of Chitosan/Quaternized Chitosan Nanofibers’ Behavior in Wound Exudate Media |
| title_fullStr | Theoretical—Experimental Approach of Chitosan/Quaternized Chitosan Nanofibers’ Behavior in Wound Exudate Media |
| title_full_unstemmed | Theoretical—Experimental Approach of Chitosan/Quaternized Chitosan Nanofibers’ Behavior in Wound Exudate Media |
| title_short | Theoretical—Experimental Approach of Chitosan/Quaternized Chitosan Nanofibers’ Behavior in Wound Exudate Media |
| title_sort | theoretical experimental approach of chitosan quaternized chitosan nanofibers behavior in wound exudate media |
| topic | chitosan fibers biomaterials engineering nanomaterials composite nanomaterials enzymatic degradation |
| url | https://www.mdpi.com/1999-4923/15/12/2722 |
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