Isolation of pathogenesis-related proteins from TMV-infected tobacco and their influence on infectivity of TMV
The composition of pathogenesis-related proteins (PR-proteins) in the intercellular fluid (ICF) and leaf tissue of the hypersensitive tobacco cultivar Xanthi-nc inoculated with Tobacco mosaic virus (TMV), and their inhibitory influence on TMV multiplication were studied. The ICF PR-proteins of infec...
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| Format: | Article |
| Language: | English |
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Czech Academy of Agricultural Sciences
2005-06-01
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| Series: | Plant Protection Science |
| Subjects: | |
| Online Access: | https://pps.agriculturejournals.cz/artkey/pps-200502-0002_isolation-of-pathogenesis-related-proteins-from-tmv-infected-tobacco-and-their-influence-on-infectivity-of-tmv.php |
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| author | Milada Šindelářová Luděk Šindelář |
| author_facet | Milada Šindelářová Luděk Šindelář |
| author_sort | Milada Šindelářová |
| collection | DOAJ |
| description | The composition of pathogenesis-related proteins (PR-proteins) in the intercellular fluid (ICF) and leaf tissue of the hypersensitive tobacco cultivar Xanthi-nc inoculated with Tobacco mosaic virus (TMV), and their inhibitory influence on TMV multiplication were studied. The ICF PR-proteins of infected plants were separated after solubilisation by decreasing gradient of ammonium sulphate, the cell PR-proteins were separated after acidic homogenisation of leaf tissues. The ICF and cell PR-proteins were further purified by ion exchange chromatography on DEAE cellulose. Using discontinuous non-denaturating polyacrylamide gel electrophoresis of DEAE cellulose fractions the PR-proteins were detected. Their molecular weights were estimated by SDS-PAGE. The ICF and cell proteins of infected leaves included PR-proteins of the molecular weights 15-16 kDa (Group 1), 27-28 kDa (Group 3: chitinases) and 36-40 kDa (Group 2a: ڨ-1,3-glucanases). Fractions with different PR-proteins were tested for their effect on infectivity of TMV. Particularly the PR3 and PR2a proteins seem to decrease the infectivity of TMV. |
| first_indexed | 2024-04-10T07:56:16Z |
| format | Article |
| id | doaj.art-4bf4c2990f7941eaac6f9bb375e57b8d |
| institution | Directory Open Access Journal |
| issn | 1212-2580 1805-9341 |
| language | English |
| last_indexed | 2024-04-10T07:56:16Z |
| publishDate | 2005-06-01 |
| publisher | Czech Academy of Agricultural Sciences |
| record_format | Article |
| series | Plant Protection Science |
| spelling | doaj.art-4bf4c2990f7941eaac6f9bb375e57b8d2023-02-23T04:03:30ZengCzech Academy of Agricultural SciencesPlant Protection Science1212-25801805-93412005-06-01412525710.17221/2747-PPSpps-200502-0002Isolation of pathogenesis-related proteins from TMV-infected tobacco and their influence on infectivity of TMVMilada Šindelářová0Luděk Šindelář1Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech RepublicInstitute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech RepublicThe composition of pathogenesis-related proteins (PR-proteins) in the intercellular fluid (ICF) and leaf tissue of the hypersensitive tobacco cultivar Xanthi-nc inoculated with Tobacco mosaic virus (TMV), and their inhibitory influence on TMV multiplication were studied. The ICF PR-proteins of infected plants were separated after solubilisation by decreasing gradient of ammonium sulphate, the cell PR-proteins were separated after acidic homogenisation of leaf tissues. The ICF and cell PR-proteins were further purified by ion exchange chromatography on DEAE cellulose. Using discontinuous non-denaturating polyacrylamide gel electrophoresis of DEAE cellulose fractions the PR-proteins were detected. Their molecular weights were estimated by SDS-PAGE. The ICF and cell proteins of infected leaves included PR-proteins of the molecular weights 15-16 kDa (Group 1), 27-28 kDa (Group 3: chitinases) and 36-40 kDa (Group 2a: ڨ-1,3-glucanases). Fractions with different PR-proteins were tested for their effect on infectivity of TMV. Particularly the PR3 and PR2a proteins seem to decrease the infectivity of TMV.https://pps.agriculturejournals.cz/artkey/pps-200502-0002_isolation-of-pathogenesis-related-proteins-from-tmv-infected-tobacco-and-their-influence-on-infectivity-of-tmv.phppr-proteinspagetobacco mosaic viru |
| spellingShingle | Milada Šindelářová Luděk Šindelář Isolation of pathogenesis-related proteins from TMV-infected tobacco and their influence on infectivity of TMV Plant Protection Science pr-proteins page tobacco mosaic viru |
| title | Isolation of pathogenesis-related proteins from TMV-infected tobacco and their influence on infectivity of TMV |
| title_full | Isolation of pathogenesis-related proteins from TMV-infected tobacco and their influence on infectivity of TMV |
| title_fullStr | Isolation of pathogenesis-related proteins from TMV-infected tobacco and their influence on infectivity of TMV |
| title_full_unstemmed | Isolation of pathogenesis-related proteins from TMV-infected tobacco and their influence on infectivity of TMV |
| title_short | Isolation of pathogenesis-related proteins from TMV-infected tobacco and their influence on infectivity of TMV |
| title_sort | isolation of pathogenesis related proteins from tmv infected tobacco and their influence on infectivity of tmv |
| topic | pr-proteins page tobacco mosaic viru |
| url | https://pps.agriculturejournals.cz/artkey/pps-200502-0002_isolation-of-pathogenesis-related-proteins-from-tmv-infected-tobacco-and-their-influence-on-infectivity-of-tmv.php |
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