Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe
Morphology-based identification of North Atlantic Sebastes has long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management and seafood labelling. North Atlantic Sebastes comprises of four species, commonly known a...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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PeerJ Inc.
2017-10-01
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| Series: | PeerJ |
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| Online Access: | https://peerj.com/articles/3746.pdf |
| _version_ | 1797417857116536832 |
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| author | Peter Shum Lauren Moore Christophe Pampoulie Cristina Di Muri Sara Vandamme Stefano Mariani |
| author_facet | Peter Shum Lauren Moore Christophe Pampoulie Cristina Di Muri Sara Vandamme Stefano Mariani |
| author_sort | Peter Shum |
| collection | DOAJ |
| description | Morphology-based identification of North Atlantic Sebastes has long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management and seafood labelling. North Atlantic Sebastes comprises of four species, commonly known as ‘redfish’, but little is known about the number, identity and labelling accuracy of redfish species sold across Europe. We used a molecular approach to identify redfish species from ‘blind’ specimens to evaluate the performance of the Barcode of Life (BOLD) and Genbank databases, as well as carrying out a market product accuracy survey from retailers across Europe. The conventional BOLD approach proved ambiguous, and phylogenetic analysis based on mtDNA control region sequences provided a higher resolution for species identification. By sampling market products from four countries, we found the presence of two species of redfish (S. norvegicus and S. mentella) and one unidentified Pacific rockfish marketed in Europe. Furthermore, public databases revealed the existence of inaccurate reference sequences, likely stemming from species misidentification from previous studies, which currently hinders the efficacy of DNA methods for the identification of Sebastes market samples. |
| first_indexed | 2024-03-09T06:25:46Z |
| format | Article |
| id | doaj.art-4bf60445a5b04e1e8c5d7db0990ac4e5 |
| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:25:46Z |
| publishDate | 2017-10-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-4bf60445a5b04e1e8c5d7db0990ac4e52023-12-03T11:20:57ZengPeerJ Inc.PeerJ2167-83592017-10-015e374610.7717/peerj.3746Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in EuropePeter Shum0Lauren Moore1Christophe Pampoulie2Cristina Di Muri3Sara Vandamme4Stefano Mariani5Hopkins Marine Station, Stanford University, Pacific Grove, CA, USASchool of Environment & Life Sciences, University of Salford, Salford, Greater Manchester, United KingdomMarine and Freshwater Research Institute, Reykjavík, IcelandSchool of Environment & Life Sciences, University of Salford, Salford, Greater Manchester, United KingdomSchool of Environment & Life Sciences, University of Salford, Salford, Greater Manchester, United KingdomSchool of Environment & Life Sciences, University of Salford, Salford, Greater Manchester, United KingdomMorphology-based identification of North Atlantic Sebastes has long been controversial and misidentification may produce misleading data, with cascading consequences that negatively affect fisheries management and seafood labelling. North Atlantic Sebastes comprises of four species, commonly known as ‘redfish’, but little is known about the number, identity and labelling accuracy of redfish species sold across Europe. We used a molecular approach to identify redfish species from ‘blind’ specimens to evaluate the performance of the Barcode of Life (BOLD) and Genbank databases, as well as carrying out a market product accuracy survey from retailers across Europe. The conventional BOLD approach proved ambiguous, and phylogenetic analysis based on mtDNA control region sequences provided a higher resolution for species identification. By sampling market products from four countries, we found the presence of two species of redfish (S. norvegicus and S. mentella) and one unidentified Pacific rockfish marketed in Europe. Furthermore, public databases revealed the existence of inaccurate reference sequences, likely stemming from species misidentification from previous studies, which currently hinders the efficacy of DNA methods for the identification of Sebastes market samples.https://peerj.com/articles/3746.pdfSebastesRedfishBarcodingMislabellingSeafoodCOI |
| spellingShingle | Peter Shum Lauren Moore Christophe Pampoulie Cristina Di Muri Sara Vandamme Stefano Mariani Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe PeerJ Sebastes Redfish Barcoding Mislabelling Seafood COI |
| title | Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
| title_full | Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
| title_fullStr | Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
| title_full_unstemmed | Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
| title_short | Harnessing mtDNA variation to resolve ambiguity in ‘Redfish’ sold in Europe |
| title_sort | harnessing mtdna variation to resolve ambiguity in redfish sold in europe |
| topic | Sebastes Redfish Barcoding Mislabelling Seafood COI |
| url | https://peerj.com/articles/3746.pdf |
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