The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth
IntroductionTo survive in various hostile environments, two-component system is an adaptive mechanism for diverse bacteria. Activity of the CpxA/CpxR two-component system contributes to coping with different stimuli, such as pH, osmotic and heat stress.MethodsHowever, the role of the CpxA/CpxR syste...
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| Format: | Article |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1079390/full |
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| author | Qing Yao Tingting Xie Yu Fu Jiajia Wan Wendie Zhang Xuejun Gao Jing Huang Diangang Sun Fuxian Zhang Weicheng Bei Liancheng Lei Liancheng Lei Feng Liu |
| author_facet | Qing Yao Tingting Xie Yu Fu Jiajia Wan Wendie Zhang Xuejun Gao Jing Huang Diangang Sun Fuxian Zhang Weicheng Bei Liancheng Lei Liancheng Lei Feng Liu |
| author_sort | Qing Yao |
| collection | DOAJ |
| description | IntroductionTo survive in various hostile environments, two-component system is an adaptive mechanism for diverse bacteria. Activity of the CpxA/CpxR two-component system contributes to coping with different stimuli, such as pH, osmotic and heat stress.MethodsHowever, the role of the CpxA/CpxR system in cold resistance is little-known. In this study, we showed that CpxA/CpxRwas critical for A. pleuropneumoniae growth under cold stress.Resultsβ-Galactosidaseanalysis showed that CpxA/CpxR positively regulated the predicted cold stress gene cspC. The mutant for cold stress gene cspC was impaired in the optimal growth of A. pleuropneumoniae under cold stress. Furthermore, electrophoretic mobility shift assays demonstrated that CpxR-P could directly regulate the transcription of the cold stress gene cspC.DiscussionThese results presented in this study illustrated that the CpxA/CpxR system plays an important role in cold resistance by upregulating expression of CspC. The data give new insights into how A. pleuropneumoniae survives in cold stress. |
| first_indexed | 2024-04-11T05:27:00Z |
| format | Article |
| id | doaj.art-4bfb16502d16488fa4ce58d8f7c52a5e |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T05:27:00Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-4bfb16502d16488fa4ce58d8f7c52a5e2022-12-23T05:29:50ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-12-011310.3389/fmicb.2022.10793901079390The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growthQing Yao0Tingting Xie1Yu Fu2Jiajia Wan3Wendie Zhang4Xuejun Gao5Jing Huang6Diangang Sun7Fuxian Zhang8Weicheng Bei9Liancheng Lei10Liancheng Lei11Feng Liu12College of Animal Sciences, Yangtze University, Jingzhou, Hubei, ChinaCollege of Animal Sciences, Yangtze University, Jingzhou, Hubei, ChinaCollege of Animal Sciences, Yangtze University, Jingzhou, Hubei, ChinaCollege of Animal Sciences, Yangtze University, Jingzhou, Hubei, ChinaCollege of Animal Sciences, Yangtze University, Jingzhou, Hubei, ChinaCollege of Animal Sciences, Yangtze University, Jingzhou, Hubei, ChinaSchool of Foreign Languages, Zhejiang Gongshang University, Hangzhou, Zhejiang, ChinaCollege of Animal Sciences, Yangtze University, Jingzhou, Hubei, ChinaCollege of Animal Sciences, Yangtze University, Jingzhou, Hubei, ChinaState Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, ChinaCollege of Animal Sciences, Yangtze University, Jingzhou, Hubei, ChinaCollege of Veterinary Medicine, Jilin University, Changchun, ChinaCollege of Animal Sciences, Yangtze University, Jingzhou, Hubei, ChinaIntroductionTo survive in various hostile environments, two-component system is an adaptive mechanism for diverse bacteria. Activity of the CpxA/CpxR two-component system contributes to coping with different stimuli, such as pH, osmotic and heat stress.MethodsHowever, the role of the CpxA/CpxR system in cold resistance is little-known. In this study, we showed that CpxA/CpxRwas critical for A. pleuropneumoniae growth under cold stress.Resultsβ-Galactosidaseanalysis showed that CpxA/CpxR positively regulated the predicted cold stress gene cspC. The mutant for cold stress gene cspC was impaired in the optimal growth of A. pleuropneumoniae under cold stress. Furthermore, electrophoretic mobility shift assays demonstrated that CpxR-P could directly regulate the transcription of the cold stress gene cspC.DiscussionThese results presented in this study illustrated that the CpxA/CpxR system plays an important role in cold resistance by upregulating expression of CspC. The data give new insights into how A. pleuropneumoniae survives in cold stress.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1079390/fullActinobacillus pleuropneumoniaetwo-component systemCpxA/CpxRcold stresscspC |
| spellingShingle | Qing Yao Tingting Xie Yu Fu Jiajia Wan Wendie Zhang Xuejun Gao Jing Huang Diangang Sun Fuxian Zhang Weicheng Bei Liancheng Lei Liancheng Lei Feng Liu The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth Frontiers in Microbiology Actinobacillus pleuropneumoniae two-component system CpxA/CpxR cold stress cspC |
| title | The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth |
| title_full | The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth |
| title_fullStr | The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth |
| title_full_unstemmed | The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth |
| title_short | The CpxA/CpxR two-component system mediates regulation of Actinobacillus pleuropneumoniae cold growth |
| title_sort | cpxa cpxr two component system mediates regulation of actinobacillus pleuropneumoniae cold growth |
| topic | Actinobacillus pleuropneumoniae two-component system CpxA/CpxR cold stress cspC |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1079390/full |
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