Bacteria Patterns on Tonsillar Surface and Tonsillar Core Tissue among Patients Scheduled for Tonsillectomy at Bugando Medical Centre, Mwanza, Tanzania
Background: Tonsillitis is an inflammation of the tonsils due to either viruses or bacteria. Here, we report the bacteria patterns on the tonsillar surface and tonsillar core tissue among patients scheduled for tonsillectomy at Bugando Medical Centre (BMC), Mwanza Tanzania. Methods: The study includ...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-11-01
|
| Series: | Pathogens |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-0817/10/12/1560 |
| _version_ | 1797501700700897280 |
|---|---|
| author | Gustave Buname Gapto Aristides Kiwale Martha F. Mushi Vitus Silago Peter Rambau Stephen E. Mshana |
| author_facet | Gustave Buname Gapto Aristides Kiwale Martha F. Mushi Vitus Silago Peter Rambau Stephen E. Mshana |
| author_sort | Gustave Buname |
| collection | DOAJ |
| description | Background: Tonsillitis is an inflammation of the tonsils due to either viruses or bacteria. Here, we report the bacteria patterns on the tonsillar surface and tonsillar core tissue among patients scheduled for tonsillectomy at Bugando Medical Centre (BMC), Mwanza Tanzania. Methods: The study included 120 patients planned for tonsillectomy between April and July 2019. Swab samples from tonsillar surface pre-tonsillectomy and core post-tonsillectomy were collected. Culture was performed following the microbiology laboratory standard operating procedures. Data analysis was completed using STATA version 13, as per the study objectives. Results: The slight majority of participants were males (73; 60.83%) with median age of 6 years (interquartile range 4–11). The proportion of positive culture growth was higher on the surface than in core swab samples: 65 (54.2%) vs. 42 (35.0%), <i>p</i> = 0.003. The commonest bacterial pathogen detected from the surface and core were <i>S. aureus</i> in 29 (40.3%) and 22 (51.2%) participants, followed by <i>S. pyogenes</i> in 17 (23.6%) and 11 (25.6%), respectively. Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) was observed in 20/51 (39%) of isolates. <i>Streptococcus pyogenes</i> resistance to macrolides ranged from 8.3% for core isolates to 35.3% for surface isolates. Features suggestive of tonsillitis on histology were reported in 83 (73.5%) samples. Conclusion: More than two-thirds of patients undergoing tonsillectomy had a positive culture for possible bacterial pathogens. <i>Staphylococcus aureus</i> and <i>Streptococcus pyogenes</i> were the predominant bacteria detected with more than one third of <i>Staphylococcus aureus</i> being MRSA. More studies to investigate the treatment outcome of these patients are highly recommended. |
| first_indexed | 2024-03-10T03:22:20Z |
| format | Article |
| id | doaj.art-4bfe7dc3fc304aa9bbe7bf5013dd945e |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T03:22:20Z |
| publishDate | 2021-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-4bfe7dc3fc304aa9bbe7bf5013dd945e2023-11-23T10:00:39ZengMDPI AGPathogens2076-08172021-11-011012156010.3390/pathogens10121560Bacteria Patterns on Tonsillar Surface and Tonsillar Core Tissue among Patients Scheduled for Tonsillectomy at Bugando Medical Centre, Mwanza, TanzaniaGustave Buname0Gapto Aristides Kiwale1Martha F. Mushi2Vitus Silago3Peter Rambau4Stephen E. Mshana5Department of ENT, Bugando Medical Centre, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza P.O. Box 1464, TanzaniaDepartment of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza P.O. Box 1464, TanzaniaDepartment of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza P.O. Box 1464, TanzaniaDepartment of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza P.O. Box 1464, TanzaniaDepartment of Pathology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza P.O. Box 1464, TanzaniaDepartment of Microbiology and Immunology, Weill Bugando School of Medicine, Catholic University of Health and Allied Sciences, Mwanza P.O. Box 1464, TanzaniaBackground: Tonsillitis is an inflammation of the tonsils due to either viruses or bacteria. Here, we report the bacteria patterns on the tonsillar surface and tonsillar core tissue among patients scheduled for tonsillectomy at Bugando Medical Centre (BMC), Mwanza Tanzania. Methods: The study included 120 patients planned for tonsillectomy between April and July 2019. Swab samples from tonsillar surface pre-tonsillectomy and core post-tonsillectomy were collected. Culture was performed following the microbiology laboratory standard operating procedures. Data analysis was completed using STATA version 13, as per the study objectives. Results: The slight majority of participants were males (73; 60.83%) with median age of 6 years (interquartile range 4–11). The proportion of positive culture growth was higher on the surface than in core swab samples: 65 (54.2%) vs. 42 (35.0%), <i>p</i> = 0.003. The commonest bacterial pathogen detected from the surface and core were <i>S. aureus</i> in 29 (40.3%) and 22 (51.2%) participants, followed by <i>S. pyogenes</i> in 17 (23.6%) and 11 (25.6%), respectively. Methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) was observed in 20/51 (39%) of isolates. <i>Streptococcus pyogenes</i> resistance to macrolides ranged from 8.3% for core isolates to 35.3% for surface isolates. Features suggestive of tonsillitis on histology were reported in 83 (73.5%) samples. Conclusion: More than two-thirds of patients undergoing tonsillectomy had a positive culture for possible bacterial pathogens. <i>Staphylococcus aureus</i> and <i>Streptococcus pyogenes</i> were the predominant bacteria detected with more than one third of <i>Staphylococcus aureus</i> being MRSA. More studies to investigate the treatment outcome of these patients are highly recommended.https://www.mdpi.com/2076-0817/10/12/1560tonsillarTanzaniacoresurfacetonsillectomybacteria |
| spellingShingle | Gustave Buname Gapto Aristides Kiwale Martha F. Mushi Vitus Silago Peter Rambau Stephen E. Mshana Bacteria Patterns on Tonsillar Surface and Tonsillar Core Tissue among Patients Scheduled for Tonsillectomy at Bugando Medical Centre, Mwanza, Tanzania Pathogens tonsillar Tanzania core surface tonsillectomy bacteria |
| title | Bacteria Patterns on Tonsillar Surface and Tonsillar Core Tissue among Patients Scheduled for Tonsillectomy at Bugando Medical Centre, Mwanza, Tanzania |
| title_full | Bacteria Patterns on Tonsillar Surface and Tonsillar Core Tissue among Patients Scheduled for Tonsillectomy at Bugando Medical Centre, Mwanza, Tanzania |
| title_fullStr | Bacteria Patterns on Tonsillar Surface and Tonsillar Core Tissue among Patients Scheduled for Tonsillectomy at Bugando Medical Centre, Mwanza, Tanzania |
| title_full_unstemmed | Bacteria Patterns on Tonsillar Surface and Tonsillar Core Tissue among Patients Scheduled for Tonsillectomy at Bugando Medical Centre, Mwanza, Tanzania |
| title_short | Bacteria Patterns on Tonsillar Surface and Tonsillar Core Tissue among Patients Scheduled for Tonsillectomy at Bugando Medical Centre, Mwanza, Tanzania |
| title_sort | bacteria patterns on tonsillar surface and tonsillar core tissue among patients scheduled for tonsillectomy at bugando medical centre mwanza tanzania |
| topic | tonsillar Tanzania core surface tonsillectomy bacteria |
| url | https://www.mdpi.com/2076-0817/10/12/1560 |
| work_keys_str_mv | AT gustavebuname bacteriapatternsontonsillarsurfaceandtonsillarcoretissueamongpatientsscheduledfortonsillectomyatbugandomedicalcentremwanzatanzania AT gaptoaristideskiwale bacteriapatternsontonsillarsurfaceandtonsillarcoretissueamongpatientsscheduledfortonsillectomyatbugandomedicalcentremwanzatanzania AT marthafmushi bacteriapatternsontonsillarsurfaceandtonsillarcoretissueamongpatientsscheduledfortonsillectomyatbugandomedicalcentremwanzatanzania AT vitussilago bacteriapatternsontonsillarsurfaceandtonsillarcoretissueamongpatientsscheduledfortonsillectomyatbugandomedicalcentremwanzatanzania AT peterrambau bacteriapatternsontonsillarsurfaceandtonsillarcoretissueamongpatientsscheduledfortonsillectomyatbugandomedicalcentremwanzatanzania AT stephenemshana bacteriapatternsontonsillarsurfaceandtonsillarcoretissueamongpatientsscheduledfortonsillectomyatbugandomedicalcentremwanzatanzania |