Efficient production of protein complexes in mammalian cells using a poxvirus vector
The production of full length, biologically active proteins in mammalian cells is critical for a wide variety of purposes ranging from structural studies to preparation of subunit vaccines. Prior research has shown that Modified vaccinia virus Ankara encoding the bacteriophage T7 RNA polymerase (MVA...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754296/?tool=EBI |
| _version_ | 1828113806412218368 |
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| author | Robert Drillien Karine Pradeau-Aubreton Julien Batisse Joëlle Mezher Emma Schenckbecher Justine Marguin Eric Ennifar Marc Ruff |
| author_facet | Robert Drillien Karine Pradeau-Aubreton Julien Batisse Joëlle Mezher Emma Schenckbecher Justine Marguin Eric Ennifar Marc Ruff |
| author_sort | Robert Drillien |
| collection | DOAJ |
| description | The production of full length, biologically active proteins in mammalian cells is critical for a wide variety of purposes ranging from structural studies to preparation of subunit vaccines. Prior research has shown that Modified vaccinia virus Ankara encoding the bacteriophage T7 RNA polymerase (MVA-T7) is particularly suitable for high level expression of proteins upon infection of mammalian cells. The expression system is safe for users and 10–50 mg of full length, biologically active proteins may be obtained in their native state, from a few litres of infected cell cultures. Here we report further improvements which allow an increase in the ease and speed of recombinant virus isolation, the scale-up of protein production and the simultaneous synthesis of several polypeptides belonging to a protein complex using a single virus vector. Isolation of MVA-T7 viruses encoding foreign proteins was simplified by combining positive selection for virus recombinants and negative selection against parental virus, a process which eliminated the need for tedious plaque purification. Scale-up of protein production was achieved by infecting a BHK 21 suspension cell line and inducing protein expression with previously infected cells instead of virus, thus saving time and effort in handling virus stocks. Protein complexes were produced from infected cells by concatenating the Tobacco Etch Virus (TEV) N1A protease sequence with each of the genes of the complex into a single ORF, each gene being separated from the other by twin TEV protease cleavage sites. We report the application of these methods to the production of a complex formed on the one hand between the HIV-1 integrase and its cell partner LEDGF and on the other between the HIV-1 VIF protein and its cell partners APOBEC3G, CBFβ, Elo B and Elo C. The strategies developed in this study should be valuable for the overexpression and subsequent purification of numerous protein complexes. |
| first_indexed | 2024-04-11T12:15:59Z |
| format | Article |
| id | doaj.art-4bffa090bb394c638e1fc7682e2c2baa |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-11T12:15:59Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4bffa090bb394c638e1fc7682e2c2baa2022-12-22T04:24:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011712Efficient production of protein complexes in mammalian cells using a poxvirus vectorRobert DrillienKarine Pradeau-AubretonJulien BatisseJoëlle MezherEmma SchenckbecherJustine MarguinEric EnnifarMarc RuffThe production of full length, biologically active proteins in mammalian cells is critical for a wide variety of purposes ranging from structural studies to preparation of subunit vaccines. Prior research has shown that Modified vaccinia virus Ankara encoding the bacteriophage T7 RNA polymerase (MVA-T7) is particularly suitable for high level expression of proteins upon infection of mammalian cells. The expression system is safe for users and 10–50 mg of full length, biologically active proteins may be obtained in their native state, from a few litres of infected cell cultures. Here we report further improvements which allow an increase in the ease and speed of recombinant virus isolation, the scale-up of protein production and the simultaneous synthesis of several polypeptides belonging to a protein complex using a single virus vector. Isolation of MVA-T7 viruses encoding foreign proteins was simplified by combining positive selection for virus recombinants and negative selection against parental virus, a process which eliminated the need for tedious plaque purification. Scale-up of protein production was achieved by infecting a BHK 21 suspension cell line and inducing protein expression with previously infected cells instead of virus, thus saving time and effort in handling virus stocks. Protein complexes were produced from infected cells by concatenating the Tobacco Etch Virus (TEV) N1A protease sequence with each of the genes of the complex into a single ORF, each gene being separated from the other by twin TEV protease cleavage sites. We report the application of these methods to the production of a complex formed on the one hand between the HIV-1 integrase and its cell partner LEDGF and on the other between the HIV-1 VIF protein and its cell partners APOBEC3G, CBFβ, Elo B and Elo C. The strategies developed in this study should be valuable for the overexpression and subsequent purification of numerous protein complexes.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754296/?tool=EBI |
| spellingShingle | Robert Drillien Karine Pradeau-Aubreton Julien Batisse Joëlle Mezher Emma Schenckbecher Justine Marguin Eric Ennifar Marc Ruff Efficient production of protein complexes in mammalian cells using a poxvirus vector PLoS ONE |
| title | Efficient production of protein complexes in mammalian cells using a poxvirus vector |
| title_full | Efficient production of protein complexes in mammalian cells using a poxvirus vector |
| title_fullStr | Efficient production of protein complexes in mammalian cells using a poxvirus vector |
| title_full_unstemmed | Efficient production of protein complexes in mammalian cells using a poxvirus vector |
| title_short | Efficient production of protein complexes in mammalian cells using a poxvirus vector |
| title_sort | efficient production of protein complexes in mammalian cells using a poxvirus vector |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9754296/?tool=EBI |
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