Analysis of mRNA Decay Intermediates in Bacillus subtilis 3′ Exoribonuclease and RNA Helicase Mutant Strains

Analysis of mRNA Decay Intermediates in Bacillus subtilis 3′ Exoribonuclease and RNA Helicase Mutant Strains

ABSTRACT The Bacillus subtilis genome encodes four 3′ exoribonucleases: polynucleotide phosphorylase (PNPase), RNase R, RNase PH, and YhaM. Previous work showed that PNPase, encoded by the pnpA gene, is the major 3′ exonuclease involved in mRNA turnover; in a pnpA deletion strain, numerous mRNA deca...

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Bibliographic Details
Main Authors: Shivani Chhabra, Zachary F. Mandell, Bo Liu, Paul Babitzke, David H. Bechhofer
Format: Article
Language:English
Published: American Society for Microbiology 2022-04-01
Series:mBio
Subjects:
Bacillus subtilis
mRNA decay
3′ exoribonucleases
RNA helicase
PNPase
Term-seq
Online Access:https://journals.asm.org/doi/10.1128/mbio.00400-22

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