Anti-diabetic activity of aerial parts of Sarcopoterium spinosum
Abstract Background Sarcopoterium spinosum (S. spinosum) is used by Bedouin medicinal practitioners for the treatment of diabetes. While the anti-diabetic activity of S. spinosum root extract was validated in previous studies, the activity of aerial parts of the same plants has not been elucidated y...
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| Format: | Article |
| Language: | English |
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BMC
2017-07-01
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| Series: | BMC Complementary and Alternative Medicine |
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| Online Access: | http://link.springer.com/article/10.1186/s12906-017-1860-7 |
| _version_ | 1818891537823563776 |
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| author | Uriel Elyasiyan Adi Nudel Nir Skalka Konstantin Rozenberg Elyashiv Drori Rachela Oppenheimer Zohar Kerem Tovit Rosenzweig |
| author_facet | Uriel Elyasiyan Adi Nudel Nir Skalka Konstantin Rozenberg Elyashiv Drori Rachela Oppenheimer Zohar Kerem Tovit Rosenzweig |
| author_sort | Uriel Elyasiyan |
| collection | DOAJ |
| description | Abstract Background Sarcopoterium spinosum (S. spinosum) is used by Bedouin medicinal practitioners for the treatment of diabetes. While the anti-diabetic activity of S. spinosum root extract was validated in previous studies, the activity of aerial parts of the same plants has not been elucidated yet. The aim of this study was to clarify the glucose lowering properties of the aerial parts of the shrub. Methods Anti-diabetic properties were evaluated by measuring the activity of carbohydrate digesting enzymes, glucose uptake into 3 T3-L1 adipocytes, and insulin secretion. Insulin signaling cascade was followed in L6 myotubes using Western blot and PathScan analysis. Results Activity of α-amylase and α-glucosidase was inhibited by extracts of all S. spinosum organs. Basal and glucose-induced insulin secretion was measured in Min6 cells and found to be enhanced as well. Glucose uptake was induced by all S. spinosum extracts, with roots found to be the most effective and fruits the least. The effect of S. spinosum on Akt phosphorylation was minor compared to insulin effect. However, GSK3β and PRAS40, which are downstream elements of the insulin cascade, were found to be highly phosphorylated by S. spinosum extracts. Inhibition of PI3K and Akt, but not AMPK and ERK, abrogated the induction of glucose uptake by the aerial parts of the shrub. Conclusion The aerial organs of S. spinosum have anti-diabetic properties and may be used as a basis for the development of dietary supplements or to identify new agents for the treatment of type 2 diabetes. |
| first_indexed | 2024-12-19T17:42:23Z |
| format | Article |
| id | doaj.art-4c03fb0562ff44e4a1058dbb626974ba |
| institution | Directory Open Access Journal |
| issn | 1472-6882 |
| language | English |
| last_indexed | 2024-12-19T17:42:23Z |
| publishDate | 2017-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary and Alternative Medicine |
| spelling | doaj.art-4c03fb0562ff44e4a1058dbb626974ba2022-12-21T20:12:10ZengBMCBMC Complementary and Alternative Medicine1472-68822017-07-0117111210.1186/s12906-017-1860-7Anti-diabetic activity of aerial parts of Sarcopoterium spinosumUriel Elyasiyan0Adi Nudel1Nir Skalka2Konstantin Rozenberg3Elyashiv Drori4Rachela Oppenheimer5Zohar Kerem6Tovit Rosenzweig7Department of Molecular Biology, Department of Nutritional Studies, Ariel UniversityInstitute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of JerusalemDepartment of Molecular Biology, Department of Nutritional Studies, Ariel UniversityDepartment of Molecular Biology, Department of Nutritional Studies, Ariel UniversitySamaria and Jordan Rift R&D CenterDiabest Botanical Drugs Ltd.Institute of Biochemistry, Food Science and Nutrition, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of JerusalemDepartment of Molecular Biology, Department of Nutritional Studies, Ariel UniversityAbstract Background Sarcopoterium spinosum (S. spinosum) is used by Bedouin medicinal practitioners for the treatment of diabetes. While the anti-diabetic activity of S. spinosum root extract was validated in previous studies, the activity of aerial parts of the same plants has not been elucidated yet. The aim of this study was to clarify the glucose lowering properties of the aerial parts of the shrub. Methods Anti-diabetic properties were evaluated by measuring the activity of carbohydrate digesting enzymes, glucose uptake into 3 T3-L1 adipocytes, and insulin secretion. Insulin signaling cascade was followed in L6 myotubes using Western blot and PathScan analysis. Results Activity of α-amylase and α-glucosidase was inhibited by extracts of all S. spinosum organs. Basal and glucose-induced insulin secretion was measured in Min6 cells and found to be enhanced as well. Glucose uptake was induced by all S. spinosum extracts, with roots found to be the most effective and fruits the least. The effect of S. spinosum on Akt phosphorylation was minor compared to insulin effect. However, GSK3β and PRAS40, which are downstream elements of the insulin cascade, were found to be highly phosphorylated by S. spinosum extracts. Inhibition of PI3K and Akt, but not AMPK and ERK, abrogated the induction of glucose uptake by the aerial parts of the shrub. Conclusion The aerial organs of S. spinosum have anti-diabetic properties and may be used as a basis for the development of dietary supplements or to identify new agents for the treatment of type 2 diabetes.http://link.springer.com/article/10.1186/s12906-017-1860-7Glucose uptakeInsulin signalingMedicinal plantSarcopoterium spinosumType 2 diabetes |
| spellingShingle | Uriel Elyasiyan Adi Nudel Nir Skalka Konstantin Rozenberg Elyashiv Drori Rachela Oppenheimer Zohar Kerem Tovit Rosenzweig Anti-diabetic activity of aerial parts of Sarcopoterium spinosum BMC Complementary and Alternative Medicine Glucose uptake Insulin signaling Medicinal plant Sarcopoterium spinosum Type 2 diabetes |
| title | Anti-diabetic activity of aerial parts of Sarcopoterium spinosum |
| title_full | Anti-diabetic activity of aerial parts of Sarcopoterium spinosum |
| title_fullStr | Anti-diabetic activity of aerial parts of Sarcopoterium spinosum |
| title_full_unstemmed | Anti-diabetic activity of aerial parts of Sarcopoterium spinosum |
| title_short | Anti-diabetic activity of aerial parts of Sarcopoterium spinosum |
| title_sort | anti diabetic activity of aerial parts of sarcopoterium spinosum |
| topic | Glucose uptake Insulin signaling Medicinal plant Sarcopoterium spinosum Type 2 diabetes |
| url | http://link.springer.com/article/10.1186/s12906-017-1860-7 |
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