DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY

This study was performed in order to bring out a detailed information on growth dynamics and biochemical determination of the diatom species Nitzschia palea (Kützing) W. Smith under batch culture conditions in order to pave the way for further studies. The study material was isolated from a fresh wa...

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Main Author: Dilek Yalçın Duygu
Format: Article
Language:English
Published: Trakya University 2019-04-01
Series:Trakya University Journal of Natural Sciences
Subjects:
Online Access:https://dergipark.org.tr/en/download/article-file/680715
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author Dilek Yalçın Duygu
author_facet Dilek Yalçın Duygu
author_sort Dilek Yalçın Duygu
collection DOAJ
description This study was performed in order to bring out a detailed information on growth dynamics and biochemical determination of the diatom species Nitzschia palea (Kützing) W. Smith under batch culture conditions in order to pave the way for further studies. The study material was isolated from a fresh water sample collected from Ankara, Turkey. The diatoms were cultured in Allen medium for 168 hours and the growth dynamics were determined by cell density and dry weight analyses. Specific growth rate, duplication time of the culture and biochemical compositions were also investigated. Molecular characterization of the N. palea strain was performed by applying Fourier Transform Infrared Spectroscopy. The cell density and the dry biomass of the culture at the end of the 168 hours incubation period was determined as 2.0x106±1.0x105cells/mL and 0.212±0.041 g L-1, respectively. The algal specific growth rate was found as 0.010 h-1 at 96-h and the doubling time was calculated as 68 h-1. The protein content was measured as 41.21%, carbohydrate content as 21.74%, lipid content as 16.84% and ash content as 19.88%. These results indicated that N. palea may be used in different fields of industries, especially in biodiesel production.
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spelling doaj.art-4c04e57ddf514371a984e42d0b6dc3202023-11-08T07:27:57ZengTrakya UniversityTrakya University Journal of Natural Sciences2528-96912019-04-01201637010.23902/trkjnat.49842669DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEYDilek Yalçın Duygu0GAZI UNIVERSITY, GAZİ FACULTY OF EDUCATIONThis study was performed in order to bring out a detailed information on growth dynamics and biochemical determination of the diatom species Nitzschia palea (Kützing) W. Smith under batch culture conditions in order to pave the way for further studies. The study material was isolated from a fresh water sample collected from Ankara, Turkey. The diatoms were cultured in Allen medium for 168 hours and the growth dynamics were determined by cell density and dry weight analyses. Specific growth rate, duplication time of the culture and biochemical compositions were also investigated. Molecular characterization of the N. palea strain was performed by applying Fourier Transform Infrared Spectroscopy. The cell density and the dry biomass of the culture at the end of the 168 hours incubation period was determined as 2.0x106±1.0x105cells/mL and 0.212±0.041 g L-1, respectively. The algal specific growth rate was found as 0.010 h-1 at 96-h and the doubling time was calculated as 68 h-1. The protein content was measured as 41.21%, carbohydrate content as 21.74%, lipid content as 16.84% and ash content as 19.88%. These results indicated that N. palea may be used in different fields of industries, especially in biodiesel production.https://dergipark.org.tr/en/download/article-file/680715batch culturelipidcarbohydrateproteinashnitzschia paleaashftir
spellingShingle Dilek Yalçın Duygu
DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY
Trakya University Journal of Natural Sciences
batch culture
lipid
carbohydrate
protein
ash
nitzschia palea
ash
ftir
title DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY
title_full DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY
title_fullStr DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY
title_full_unstemmed DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY
title_short DETERMINATION OF GROWTH KINETICS AND BIOCHEMICAL COMPOSITION OF Nitzschia palea (Kützing) W. Smith ISOLATED FROM FRESHWATER SOURCES IN TURKEY
title_sort determination of growth kinetics and biochemical composition of nitzschia palea kutzing w smith isolated from freshwater sources in turkey
topic batch culture
lipid
carbohydrate
protein
ash
nitzschia palea
ash
ftir
url https://dergipark.org.tr/en/download/article-file/680715
work_keys_str_mv AT dilekyalcınduygu determinationofgrowthkineticsandbiochemicalcompositionofnitzschiapaleakutzingwsmithisolatedfromfreshwatersourcesinturkey