Temporal expression of three conserved putative microRNAs in response of Citrus × Limon to Xanthomonas citri subsp. citri and Xanthomonas fuscans subsp. Aurantifolii

Citrus canker is a widespread bacterial disease that severely affects Citrus production. Xanthomonas spp. are the causative agents of the disease, which manifests as necrotic pustule-like lesions on the plant organs including leaves, fruits, and stems. MicroRNAs (miRNAs) are well-established post...

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Bibliographic Details
Main Authors: Milad Alizadeh, Hossein Askari, Massod Soltani Najafabadi
Format: Article
Language:English
Published: Termedia Publishing House 2018-01-01
Series:BioTechnologia
Subjects:
Online Access:https://www.termedia.pl/Temporal-expression-of-three-conserved-putative-microRNAs-r-nin-response-of-Citrus-Limon-to-Xanthomonas-citri-subsp-citri-r-nand-Xanthomonas-fuscans-subsp-Aurantifolii-,85,30835,1,1.html
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Summary:Citrus canker is a widespread bacterial disease that severely affects Citrus production. Xanthomonas spp. are the causative agents of the disease, which manifests as necrotic pustule-like lesions on the plant organs including leaves, fruits, and stems. MicroRNAs (miRNAs) are well-established post-transcriptional gene expression regulators that modulate plant responses during many stress conditions. Accordingly, some bacteria-responsive miRNAs have been shown to have regulatory functions in the plant-pathogen interactions. In this study, we successfully assessed the expression patterns of three conserved miRNAs: miR159, miR167, and miR398, in Citrus × Limon (lemon) infiltrated with Xanthomonas strains A and C using stem-loop RT-qPCR. Our results showed that the expressions of miR159 and mir167 have nearly similar patterns upon inoculations, while the expression of miR398 remains constantly up-regulated after an early induction.
ISSN:0860-7796
2353-9461