Encapsulated Rose Bengal Enhances the Photodynamic Treatment of Triple-Negative Breast Cancer Cells
Among breast cancer subtypes, triple-negative breast cancer stands out as the most aggressive, with patients facing a 40% mortality rate within the initial five years. The limited treatment options and unfavourable prognosis for triple-negative patients necessitate the development of novel therapeut...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2024-01-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/2/546 |
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| author | Mir Muhammad Nasir Uddin Alina Bekmukhametova Anu Antony Shital K. Barman Jessica Houang Ming J. Wu James M. Hook Laurel George Richard Wuhrer Damia Mawad Daniel Ta Herleen Ruprai Antonio Lauto |
| author_facet | Mir Muhammad Nasir Uddin Alina Bekmukhametova Anu Antony Shital K. Barman Jessica Houang Ming J. Wu James M. Hook Laurel George Richard Wuhrer Damia Mawad Daniel Ta Herleen Ruprai Antonio Lauto |
| author_sort | Mir Muhammad Nasir Uddin |
| collection | DOAJ |
| description | Among breast cancer subtypes, triple-negative breast cancer stands out as the most aggressive, with patients facing a 40% mortality rate within the initial five years. The limited treatment options and unfavourable prognosis for triple-negative patients necessitate the development of novel therapeutic strategies. Photodynamic therapy (PDT) is an alternative treatment that can effectively target triple-negative neoplastic cells such as MDA-MB-231. In this in vitro study, we conducted a comparative analysis of the PDT killing rate of unbound Rose Bengal (RB) in solution versus RB-encapsulated chitosan nanoparticles to determine the most effective approach for inducing cytotoxicity at low laser powers (90 mW, 50 mW, 25 mW and 10 mW) and RB concentrations (50 µg/mL, 25 µg/mL, 10 µg/mL and 5 µg/mL). Intracellular singlet oxygen production and cell uptake were also determined for both treatment modalities. Dark toxicity was also assessed for normal breast cells. Despite the low laser power and concentration of nanoparticles (10 mW and 5 µg/mL), MDA-MB-231 cells experienced a substantial reduction in viability (8 ± 1%) compared to those treated with RB solution (38 ± 10%). RB nanoparticles demonstrated higher singlet oxygen production and greater uptake by cancer cells than RB solutions. Moreover, RB nanoparticles display strong cytocompatibility with normal breast cells (MCF-10A). The low activation threshold may be a crucial advantage for specifically targeting malignant cells in deep tissues. |
| first_indexed | 2024-03-08T09:48:38Z |
| format | Article |
| id | doaj.art-4c0693d494584cc09b29db2047a34e69 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-08T09:48:38Z |
| publishDate | 2024-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-4c0693d494584cc09b29db2047a34e692024-01-29T14:09:28ZengMDPI AGMolecules1420-30492024-01-0129254610.3390/molecules29020546Encapsulated Rose Bengal Enhances the Photodynamic Treatment of Triple-Negative Breast Cancer CellsMir Muhammad Nasir Uddin0Alina Bekmukhametova1Anu Antony2Shital K. Barman3Jessica Houang4Ming J. Wu5James M. Hook6Laurel George7Richard Wuhrer8Damia Mawad9Daniel Ta10Herleen Ruprai11Antonio Lauto12School of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Chemistry, University of New South Wales, Sydney, NSW 2052, AustraliaAdvanced Materials Characterisation Facility, Western Sydney University, Penrith, NSW 2750, AustraliaAdvanced Materials Characterisation Facility, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Materials Science and Engineering and Australian Centre for NanoMedicine, University of New South Wales, Kensington, NSW 2052, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaSchool of Science, Western Sydney University, Penrith, NSW 2750, AustraliaAmong breast cancer subtypes, triple-negative breast cancer stands out as the most aggressive, with patients facing a 40% mortality rate within the initial five years. The limited treatment options and unfavourable prognosis for triple-negative patients necessitate the development of novel therapeutic strategies. Photodynamic therapy (PDT) is an alternative treatment that can effectively target triple-negative neoplastic cells such as MDA-MB-231. In this in vitro study, we conducted a comparative analysis of the PDT killing rate of unbound Rose Bengal (RB) in solution versus RB-encapsulated chitosan nanoparticles to determine the most effective approach for inducing cytotoxicity at low laser powers (90 mW, 50 mW, 25 mW and 10 mW) and RB concentrations (50 µg/mL, 25 µg/mL, 10 µg/mL and 5 µg/mL). Intracellular singlet oxygen production and cell uptake were also determined for both treatment modalities. Dark toxicity was also assessed for normal breast cells. Despite the low laser power and concentration of nanoparticles (10 mW and 5 µg/mL), MDA-MB-231 cells experienced a substantial reduction in viability (8 ± 1%) compared to those treated with RB solution (38 ± 10%). RB nanoparticles demonstrated higher singlet oxygen production and greater uptake by cancer cells than RB solutions. Moreover, RB nanoparticles display strong cytocompatibility with normal breast cells (MCF-10A). The low activation threshold may be a crucial advantage for specifically targeting malignant cells in deep tissues.https://www.mdpi.com/1420-3049/29/2/546triple-negative breast cancerlaserstumoursRose Bengalreactive oxygen species |
| spellingShingle | Mir Muhammad Nasir Uddin Alina Bekmukhametova Anu Antony Shital K. Barman Jessica Houang Ming J. Wu James M. Hook Laurel George Richard Wuhrer Damia Mawad Daniel Ta Herleen Ruprai Antonio Lauto Encapsulated Rose Bengal Enhances the Photodynamic Treatment of Triple-Negative Breast Cancer Cells Molecules triple-negative breast cancer lasers tumours Rose Bengal reactive oxygen species |
| title | Encapsulated Rose Bengal Enhances the Photodynamic Treatment of Triple-Negative Breast Cancer Cells |
| title_full | Encapsulated Rose Bengal Enhances the Photodynamic Treatment of Triple-Negative Breast Cancer Cells |
| title_fullStr | Encapsulated Rose Bengal Enhances the Photodynamic Treatment of Triple-Negative Breast Cancer Cells |
| title_full_unstemmed | Encapsulated Rose Bengal Enhances the Photodynamic Treatment of Triple-Negative Breast Cancer Cells |
| title_short | Encapsulated Rose Bengal Enhances the Photodynamic Treatment of Triple-Negative Breast Cancer Cells |
| title_sort | encapsulated rose bengal enhances the photodynamic treatment of triple negative breast cancer cells |
| topic | triple-negative breast cancer lasers tumours Rose Bengal reactive oxygen species |
| url | https://www.mdpi.com/1420-3049/29/2/546 |
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