The Development of Piezo-Driven Tools for Cellular Piercing
Conventionally, intracytoplasmic sperm injection (ICSI) in the mouse is conducted with piezo-drills that use a droplet of mercury for damping. The use of mercury causes concerns of toxicity and contamination. Although Fluorinert can be used as a substitute for mercury to reduce piezo-drill’s lateral...
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| Format: | Article |
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MDPI AG
2016-10-01
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| Series: | Applied Sciences |
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| Online Access: | http://www.mdpi.com/2076-3417/6/11/314 |
| _version_ | 1818519113650143232 |
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| author | Changhai Ru Peng Pan Ruihua Chen |
| author_facet | Changhai Ru Peng Pan Ruihua Chen |
| author_sort | Changhai Ru |
| collection | DOAJ |
| description | Conventionally, intracytoplasmic sperm injection (ICSI) in the mouse is conducted with piezo-drills that use a droplet of mercury for damping. The use of mercury causes concerns of toxicity and contamination. Although Fluorinert can be used as a substitute for mercury to reduce piezo-drill’s lateral vibration, the damping effect is not as satisfactory as mercury. In this work, a modified piezo-drill without using mercury was developed for the cellular piercing of mouse oocytes. Experimentally, appropriate parameters of driving voltage and frequency were obtained for the penetration of the zona pellucida of mouse oocytes. Furthermore, the lateral vibration of the injection pipette is lower than 1 μm in deionized water, which is not observable at 400 magnificence. With the piezo-drill without using mercury, the system performs the cellular piercing of mouse oocytes with a maximum cleavage rate of 94.7% (n = 117). |
| first_indexed | 2024-12-11T01:19:24Z |
| format | Article |
| id | doaj.art-4c0a9359fcd547da9d6b3a7542863e26 |
| institution | Directory Open Access Journal |
| issn | 2076-3417 |
| language | English |
| last_indexed | 2024-12-11T01:19:24Z |
| publishDate | 2016-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Applied Sciences |
| spelling | doaj.art-4c0a9359fcd547da9d6b3a7542863e262022-12-22T01:25:44ZengMDPI AGApplied Sciences2076-34172016-10-0161131410.3390/app6110314app6110314The Development of Piezo-Driven Tools for Cellular PiercingChanghai Ru0Peng Pan1Ruihua Chen2Jiangsu Provincial Key Laboratory of Advanced Robotics, Soochow University, Suzhou 215021, ChinaJiangsu Provincial Key Laboratory of Advanced Robotics, Soochow University, Suzhou 215021, ChinaInstitute of Clinical Immunology, The First Affiliated Hospital of Soochow University, Suzhou 215006, ChinaConventionally, intracytoplasmic sperm injection (ICSI) in the mouse is conducted with piezo-drills that use a droplet of mercury for damping. The use of mercury causes concerns of toxicity and contamination. Although Fluorinert can be used as a substitute for mercury to reduce piezo-drill’s lateral vibration, the damping effect is not as satisfactory as mercury. In this work, a modified piezo-drill without using mercury was developed for the cellular piercing of mouse oocytes. Experimentally, appropriate parameters of driving voltage and frequency were obtained for the penetration of the zona pellucida of mouse oocytes. Furthermore, the lateral vibration of the injection pipette is lower than 1 μm in deionized water, which is not observable at 400 magnificence. With the piezo-drill without using mercury, the system performs the cellular piercing of mouse oocytes with a maximum cleavage rate of 94.7% (n = 117).http://www.mdpi.com/2076-3417/6/11/314modified piezo-drillpiezoelectric actuationlateral vibrationmouse oocyte piercing |
| spellingShingle | Changhai Ru Peng Pan Ruihua Chen The Development of Piezo-Driven Tools for Cellular Piercing Applied Sciences modified piezo-drill piezoelectric actuation lateral vibration mouse oocyte piercing |
| title | The Development of Piezo-Driven Tools for Cellular Piercing |
| title_full | The Development of Piezo-Driven Tools for Cellular Piercing |
| title_fullStr | The Development of Piezo-Driven Tools for Cellular Piercing |
| title_full_unstemmed | The Development of Piezo-Driven Tools for Cellular Piercing |
| title_short | The Development of Piezo-Driven Tools for Cellular Piercing |
| title_sort | development of piezo driven tools for cellular piercing |
| topic | modified piezo-drill piezoelectric actuation lateral vibration mouse oocyte piercing |
| url | http://www.mdpi.com/2076-3417/6/11/314 |
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