Adiponectin and its receptor genes' expression in response to Marek's disease virus infection of White Leghorns
Marek’s disease virus (MDV) causes T-cell lymphoma in susceptible chicken and is also related to an imbalance of the lipid metabolism. Adiponectin is a circulatory cytokine secreted from adipose tissue and exerts critical metabolic functions. Although the associations between adiponectin and disease...
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| Format: | Article |
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Elsevier
2020-09-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579120303266 |
| _version_ | 1818341458641420288 |
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| author | Ying Bai Ping Yuan Huanmin Zhang Ramesh Ramachandran Ning Yang Jiuzhou Song |
| author_facet | Ying Bai Ping Yuan Huanmin Zhang Ramesh Ramachandran Ning Yang Jiuzhou Song |
| author_sort | Ying Bai |
| collection | DOAJ |
| description | Marek’s disease virus (MDV) causes T-cell lymphoma in susceptible chicken and is also related to an imbalance of the lipid metabolism. Adiponectin is a circulatory cytokine secreted from adipose tissue and exerts critical metabolic functions. Although the associations between adiponectin and diseases, including lipid disorder and noncardiac vascular diseases, have been reported, little is known about the relationship between MDV infection and adiponectin. Here, we challenged white Leghorns from Marek’s disease (MD)-susceptible and MD-resistant lines with MDV at 7 D of age and then explored the body weight and plasma lipoprotein levels at 21 D after MDV infection. Meanwhile, adiponectin and the expression of its receptors were detected using quantitative real-time PCR and Western blot. The results showed that MDV infection induced body weight loss in all the experimental birds. Meanwhile, the concentrations of total cholesterol and high-density lipoprotein were lower after the infection, although there was no significant difference (P > 0.05). However, the infection did not affect adiponectin circulating levels in plasma. MD-susceptible birds had much lower plasma adiponectin than MD-resistant birds (P < 0.01). In abdominal fat, there was no significant difference in adiponectin mRNA level. Still, we observed a significant decrease in adiponectin protein concentration, as well as adipoR1 and adipoR2, at both mRNA and protein levels in the infected compared with the noninfected MD-susceptible chickens. In the spleen, MDV infection significantly reduced the adiponectin mRNA expression but increased the protein in MD-susceptible chickens, which decreased both adipoR1 mRNA expression and protein levels. Also interestingly, the adipoR1 mRNA expression level was significantly increased in MD-susceptible chickens in the liver after MDV infection. All findings in the present study provided interesting insights into adiponectin metabolism in chickens after MDV infection, which helps to advance the understanding of lipid metabolism in response to herpesvirus infection. |
| first_indexed | 2024-12-13T15:59:07Z |
| format | Article |
| id | doaj.art-4c0b9e5b2040422fa23fdf2fe83c6fe1 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-12-13T15:59:07Z |
| publishDate | 2020-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-4c0b9e5b2040422fa23fdf2fe83c6fe12022-12-21T23:39:13ZengElsevierPoultry Science0032-57912020-09-0199942494258Adiponectin and its receptor genes' expression in response to Marek's disease virus infection of White LeghornsYing Bai0Ping Yuan1Huanmin Zhang2Ramesh Ramachandran3Ning Yang4Jiuzhou Song5College of Life Sciences and Food Engineering, Hebei University of Engineering, Handan, 056038 P.R. ChinaDepartment of Animal & Avian Sciences, University of Maryland, College Park, MD, 20742 USAUSDA, ARS, Avian Disease and Oncology Laboratory, East Lansing, MI, 48823 USADepartment of Animal Science, Pennsylvania State University, University Park, PA, 16802 USADepartment of Animal Breeding and Genetics, College of Animal Sciences, China Agricultural, Beijing, 100193 P.R. ChinaDepartment of Animal & Avian Sciences, University of Maryland, College Park, MD, 20742 USA; Corresponding author:Marek’s disease virus (MDV) causes T-cell lymphoma in susceptible chicken and is also related to an imbalance of the lipid metabolism. Adiponectin is a circulatory cytokine secreted from adipose tissue and exerts critical metabolic functions. Although the associations between adiponectin and diseases, including lipid disorder and noncardiac vascular diseases, have been reported, little is known about the relationship between MDV infection and adiponectin. Here, we challenged white Leghorns from Marek’s disease (MD)-susceptible and MD-resistant lines with MDV at 7 D of age and then explored the body weight and plasma lipoprotein levels at 21 D after MDV infection. Meanwhile, adiponectin and the expression of its receptors were detected using quantitative real-time PCR and Western blot. The results showed that MDV infection induced body weight loss in all the experimental birds. Meanwhile, the concentrations of total cholesterol and high-density lipoprotein were lower after the infection, although there was no significant difference (P > 0.05). However, the infection did not affect adiponectin circulating levels in plasma. MD-susceptible birds had much lower plasma adiponectin than MD-resistant birds (P < 0.01). In abdominal fat, there was no significant difference in adiponectin mRNA level. Still, we observed a significant decrease in adiponectin protein concentration, as well as adipoR1 and adipoR2, at both mRNA and protein levels in the infected compared with the noninfected MD-susceptible chickens. In the spleen, MDV infection significantly reduced the adiponectin mRNA expression but increased the protein in MD-susceptible chickens, which decreased both adipoR1 mRNA expression and protein levels. Also interestingly, the adipoR1 mRNA expression level was significantly increased in MD-susceptible chickens in the liver after MDV infection. All findings in the present study provided interesting insights into adiponectin metabolism in chickens after MDV infection, which helps to advance the understanding of lipid metabolism in response to herpesvirus infection.http://www.sciencedirect.com/science/article/pii/S0032579120303266Marek’s disease virusherpesvirus infectionlipid metabolismpoultryhealth |
| spellingShingle | Ying Bai Ping Yuan Huanmin Zhang Ramesh Ramachandran Ning Yang Jiuzhou Song Adiponectin and its receptor genes' expression in response to Marek's disease virus infection of White Leghorns Poultry Science Marek’s disease virus herpesvirus infection lipid metabolism poultry health |
| title | Adiponectin and its receptor genes' expression in response to Marek's disease virus infection of White Leghorns |
| title_full | Adiponectin and its receptor genes' expression in response to Marek's disease virus infection of White Leghorns |
| title_fullStr | Adiponectin and its receptor genes' expression in response to Marek's disease virus infection of White Leghorns |
| title_full_unstemmed | Adiponectin and its receptor genes' expression in response to Marek's disease virus infection of White Leghorns |
| title_short | Adiponectin and its receptor genes' expression in response to Marek's disease virus infection of White Leghorns |
| title_sort | adiponectin and its receptor genes expression in response to marek s disease virus infection of white leghorns |
| topic | Marek’s disease virus herpesvirus infection lipid metabolism poultry health |
| url | http://www.sciencedirect.com/science/article/pii/S0032579120303266 |
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