Proteomes of Residual Tumors in Curcumin-Treated Rats Reveal Changes in Microenvironment/Malignant Cell Crosstalk in a Highly Invasive Model of Mesothelioma
Curcumin exhibits both immunomodulatory properties and anticarcinogenic effects which have been investigated in different experimental tumor models and cancer types. Its interactions with multiple signaling pathways have been documented through proteomic studies on malignant cells in culture; howeve...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/22/13732 |
| _version_ | 1797465158099927040 |
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| author | Daniel L. Pouliquen Marine Malloci Alice Boissard Cécile Henry Catherine Guette |
| author_facet | Daniel L. Pouliquen Marine Malloci Alice Boissard Cécile Henry Catherine Guette |
| author_sort | Daniel L. Pouliquen |
| collection | DOAJ |
| description | Curcumin exhibits both immunomodulatory properties and anticarcinogenic effects which have been investigated in different experimental tumor models and cancer types. Its interactions with multiple signaling pathways have been documented through proteomic studies on malignant cells in culture; however, in vivo approaches are scarce. In this study, we used a rat model of highly invasive peritoneal mesothelioma to analyze the residual tumor proteomes of curcumin-treated rats in comparison with untreated tumor-bearing rats (G1) and provide insights into the modifications in the tumor microenvironment/malignant cell crosstalk. The cross-comparing analyses of the histological sections of residual tumors from two groups of rats given curcumin twice on days 21 and 26 after the tumor challenge (G2) or four times on days 7, 9, 11 and 14 (G3), in comparison with G1, identified a common increase in caveolin-1 which linked with significant abundance changes affecting 115 other proteins. The comparison of G3 vs. G2 revealed additional features for 65 main proteins, including an increase in histidine-rich glycoprotein and highly significant abundance changes for 22 other proteins regulating the tumor microenvironment, linked with the presence of numerous activated T cells. These results highlight new features in the multiple actions of curcumin on tumor microenvironment components and cancer cell invasiveness. |
| first_indexed | 2024-03-09T18:18:29Z |
| format | Article |
| id | doaj.art-4c0dd03e86b348578f6c06b7086e0d10 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T18:18:29Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4c0dd03e86b348578f6c06b7086e0d102023-11-24T08:32:29ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-11-0123221373210.3390/ijms232213732Proteomes of Residual Tumors in Curcumin-Treated Rats Reveal Changes in Microenvironment/Malignant Cell Crosstalk in a Highly Invasive Model of MesotheliomaDaniel L. Pouliquen0Marine Malloci1Alice Boissard2Cécile Henry3Catherine Guette4Université d’Angers, Inserm, CNRS, Nantes Université, CRCI2NA, F-49000 Angers, FranceNantes Université, CHU Nantes, CNRS, Inserm, BioCore, US16, SFR Bonamy, F-44000 Nantes, FranceUniversité d’Angers, ICO, Inserm, CNRS, Nantes Université, CRCI2NA, F-49000 Angers, FranceUniversité d’Angers, ICO, Inserm, CNRS, Nantes Université, CRCI2NA, F-49000 Angers, FranceUniversité d’Angers, ICO, Inserm, CNRS, Nantes Université, CRCI2NA, F-49000 Angers, FranceCurcumin exhibits both immunomodulatory properties and anticarcinogenic effects which have been investigated in different experimental tumor models and cancer types. Its interactions with multiple signaling pathways have been documented through proteomic studies on malignant cells in culture; however, in vivo approaches are scarce. In this study, we used a rat model of highly invasive peritoneal mesothelioma to analyze the residual tumor proteomes of curcumin-treated rats in comparison with untreated tumor-bearing rats (G1) and provide insights into the modifications in the tumor microenvironment/malignant cell crosstalk. The cross-comparing analyses of the histological sections of residual tumors from two groups of rats given curcumin twice on days 21 and 26 after the tumor challenge (G2) or four times on days 7, 9, 11 and 14 (G3), in comparison with G1, identified a common increase in caveolin-1 which linked with significant abundance changes affecting 115 other proteins. The comparison of G3 vs. G2 revealed additional features for 65 main proteins, including an increase in histidine-rich glycoprotein and highly significant abundance changes for 22 other proteins regulating the tumor microenvironment, linked with the presence of numerous activated T cells. These results highlight new features in the multiple actions of curcumin on tumor microenvironment components and cancer cell invasiveness.https://www.mdpi.com/1422-0067/23/22/13732curcumintumor microenvironmentimmune responseproteomicsbiomarkersT cells |
| spellingShingle | Daniel L. Pouliquen Marine Malloci Alice Boissard Cécile Henry Catherine Guette Proteomes of Residual Tumors in Curcumin-Treated Rats Reveal Changes in Microenvironment/Malignant Cell Crosstalk in a Highly Invasive Model of Mesothelioma International Journal of Molecular Sciences curcumin tumor microenvironment immune response proteomics biomarkers T cells |
| title | Proteomes of Residual Tumors in Curcumin-Treated Rats Reveal Changes in Microenvironment/Malignant Cell Crosstalk in a Highly Invasive Model of Mesothelioma |
| title_full | Proteomes of Residual Tumors in Curcumin-Treated Rats Reveal Changes in Microenvironment/Malignant Cell Crosstalk in a Highly Invasive Model of Mesothelioma |
| title_fullStr | Proteomes of Residual Tumors in Curcumin-Treated Rats Reveal Changes in Microenvironment/Malignant Cell Crosstalk in a Highly Invasive Model of Mesothelioma |
| title_full_unstemmed | Proteomes of Residual Tumors in Curcumin-Treated Rats Reveal Changes in Microenvironment/Malignant Cell Crosstalk in a Highly Invasive Model of Mesothelioma |
| title_short | Proteomes of Residual Tumors in Curcumin-Treated Rats Reveal Changes in Microenvironment/Malignant Cell Crosstalk in a Highly Invasive Model of Mesothelioma |
| title_sort | proteomes of residual tumors in curcumin treated rats reveal changes in microenvironment malignant cell crosstalk in a highly invasive model of mesothelioma |
| topic | curcumin tumor microenvironment immune response proteomics biomarkers T cells |
| url | https://www.mdpi.com/1422-0067/23/22/13732 |
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