The Image–Histology Correlation of Subcutaneous mPEG-poly(Ala) Hydrogel-Embedded MIN6 Cell Grafts in Nude Mice
Previously, we have successfully used noninvasive magnetic resonance (MR) and bioluminescence imaging to detect and monitor mPEG-poly(Ala) hydrogel-embedded MIN6 cells at the subcutaneous space for up to 64 days. In this study, we further explored the histological evolution of MIN6 cell grafts and c...
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MDPI AG
2023-06-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/15/12/2584 |
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| author | Jyuhn-Huarng Juang Chen-Ling Chen Chen-Wei Kao Chen-Yi Chen Chia-Rui Shen Jiun-Jie Wang Zei-Tsan Tsai I-Ming Chu |
| author_facet | Jyuhn-Huarng Juang Chen-Ling Chen Chen-Wei Kao Chen-Yi Chen Chia-Rui Shen Jiun-Jie Wang Zei-Tsan Tsai I-Ming Chu |
| author_sort | Jyuhn-Huarng Juang |
| collection | DOAJ |
| description | Previously, we have successfully used noninvasive magnetic resonance (MR) and bioluminescence imaging to detect and monitor mPEG-poly(Ala) hydrogel-embedded MIN6 cells at the subcutaneous space for up to 64 days. In this study, we further explored the histological evolution of MIN6 cell grafts and correlated it with image findings. MIN6 cells were incubated overnight with chitosan-coated superparamagnetic iron oxide (CSPIO) and then 5 × 10<sup>6</sup> cells in the 100 μL hydrogel solution were injected subcutaneously into each nude mouse. Grafts were removed and examined the vascularization, cell growth and proliferation with anti-CD31, SMA, insulin and ki67 antibodies, respectively, at 8, 14, 21, 29 and 36 days after transplantation. All grafts were well-vascularized with prominent CD31 and SMA staining at all time points. Interestingly, insulin-positive cells and iron-positive cells were scattered in the graft at 8 and 14 days; while clusters of insulin-positive cells without iron-positive cells appeared in the grafts at 21 days and persisted thereafter, indicating neogrowth of MIN6 cells. Moreover, proliferating MIN6 cells with strong ki67 staining was observed in 21-, 29- and 36-day grafts. Our results indicate that the originally transplanted MIN6 cells proliferated from 21 days that presented distinctive bioluminescence and MR images. |
| first_indexed | 2024-03-11T02:00:53Z |
| format | Article |
| id | doaj.art-4c18c1b3692b4bc7880211da7b007e96 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-11T02:00:53Z |
| publishDate | 2023-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-4c18c1b3692b4bc7880211da7b007e962023-11-18T12:11:46ZengMDPI AGPolymers2073-43602023-06-011512258410.3390/polym15122584The Image–Histology Correlation of Subcutaneous mPEG-poly(Ala) Hydrogel-Embedded MIN6 Cell Grafts in Nude MiceJyuhn-Huarng Juang0Chen-Ling Chen1Chen-Wei Kao2Chen-Yi Chen3Chia-Rui Shen4Jiun-Jie Wang5Zei-Tsan Tsai6I-Ming Chu7Division of Endocrinology and Metabolism, Department of Internal Medicine and Center for Tissue Engineering, Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDivision of Endocrinology and Metabolism, Department of Internal Medicine and Center for Tissue Engineering, Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDivision of Endocrinology and Metabolism, Department of Internal Medicine and Center for Tissue Engineering, Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDivision of Endocrinology and Metabolism, Department of Internal Medicine and Center for Tissue Engineering, Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDepartment of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Taoyuan 33305, TaiwanDepartment of Medical Imaging and Radiological Sciences, College of Medicine, Chang Gung University, Taoyuan 33305, TaiwanMolecular Imaging Center, Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDepartment of Chemical Engineering, National Tsing Hua University, Hsinchu 300044, TaiwanPreviously, we have successfully used noninvasive magnetic resonance (MR) and bioluminescence imaging to detect and monitor mPEG-poly(Ala) hydrogel-embedded MIN6 cells at the subcutaneous space for up to 64 days. In this study, we further explored the histological evolution of MIN6 cell grafts and correlated it with image findings. MIN6 cells were incubated overnight with chitosan-coated superparamagnetic iron oxide (CSPIO) and then 5 × 10<sup>6</sup> cells in the 100 μL hydrogel solution were injected subcutaneously into each nude mouse. Grafts were removed and examined the vascularization, cell growth and proliferation with anti-CD31, SMA, insulin and ki67 antibodies, respectively, at 8, 14, 21, 29 and 36 days after transplantation. All grafts were well-vascularized with prominent CD31 and SMA staining at all time points. Interestingly, insulin-positive cells and iron-positive cells were scattered in the graft at 8 and 14 days; while clusters of insulin-positive cells without iron-positive cells appeared in the grafts at 21 days and persisted thereafter, indicating neogrowth of MIN6 cells. Moreover, proliferating MIN6 cells with strong ki67 staining was observed in 21-, 29- and 36-day grafts. Our results indicate that the originally transplanted MIN6 cells proliferated from 21 days that presented distinctive bioluminescence and MR images.https://www.mdpi.com/2073-4360/15/12/2584mPEG-poly(Ala) hydrogelMIN6 cellssubcutaneous transplantationgraft imaginggraft histology |
| spellingShingle | Jyuhn-Huarng Juang Chen-Ling Chen Chen-Wei Kao Chen-Yi Chen Chia-Rui Shen Jiun-Jie Wang Zei-Tsan Tsai I-Ming Chu The Image–Histology Correlation of Subcutaneous mPEG-poly(Ala) Hydrogel-Embedded MIN6 Cell Grafts in Nude Mice Polymers mPEG-poly(Ala) hydrogel MIN6 cells subcutaneous transplantation graft imaging graft histology |
| title | The Image–Histology Correlation of Subcutaneous mPEG-poly(Ala) Hydrogel-Embedded MIN6 Cell Grafts in Nude Mice |
| title_full | The Image–Histology Correlation of Subcutaneous mPEG-poly(Ala) Hydrogel-Embedded MIN6 Cell Grafts in Nude Mice |
| title_fullStr | The Image–Histology Correlation of Subcutaneous mPEG-poly(Ala) Hydrogel-Embedded MIN6 Cell Grafts in Nude Mice |
| title_full_unstemmed | The Image–Histology Correlation of Subcutaneous mPEG-poly(Ala) Hydrogel-Embedded MIN6 Cell Grafts in Nude Mice |
| title_short | The Image–Histology Correlation of Subcutaneous mPEG-poly(Ala) Hydrogel-Embedded MIN6 Cell Grafts in Nude Mice |
| title_sort | image histology correlation of subcutaneous mpeg poly ala hydrogel embedded min6 cell grafts in nude mice |
| topic | mPEG-poly(Ala) hydrogel MIN6 cells subcutaneous transplantation graft imaging graft histology |
| url | https://www.mdpi.com/2073-4360/15/12/2584 |
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