Anticancer activity of Curcuma aeroginosa essential oil and its nano-formulations: cytotoxicity, apoptosis and cell migration effects
Abstract Background and aims Curcuma aeruginosa, commonly known as “kha-min-dam” in Thai, holds significance in Asian traditional medicine due to its potential in treating various diseases, having properties such as anti-HIV, hepatoprotective, antimicrobial and anti-androgenic activities. This study...
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| Format: | Article |
| Language: | English |
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BMC
2024-01-01
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| Series: | BMC Complementary Medicine and Therapies |
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| Online Access: | https://doi.org/10.1186/s12906-023-04261-9 |
| _version_ | 1797363656014430208 |
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| author | Pawaret Panyajai Natsima Viriyaadhammaa Singkome Tima Sawitree Chiampanichayakul Pornngarm Dejkriengkraikul Siriporn Okonogi Songyot Anuchapreeda |
| author_facet | Pawaret Panyajai Natsima Viriyaadhammaa Singkome Tima Sawitree Chiampanichayakul Pornngarm Dejkriengkraikul Siriporn Okonogi Songyot Anuchapreeda |
| author_sort | Pawaret Panyajai |
| collection | DOAJ |
| description | Abstract Background and aims Curcuma aeruginosa, commonly known as “kha-min-dam” in Thai, holds significance in Asian traditional medicine due to its potential in treating various diseases, having properties such as anti-HIV, hepatoprotective, antimicrobial and anti-androgenic activities. This study explores the anticancer activity of C. aeruginosa essential oil (CAEO) and its nano-formulations. Methods CAEO obtained from hydrodistillation of C. aeruginosa fresh rhizomes was examined by gas chromatography mass spectroscopy. Cytotoxicity of CAEO was determined in leukaemic K562 and breast cancer MCF-7 cell lines using an MTT assay. Cell cycle analysis and cell apoptosis were determined by flow cytometry. Cell migration was studied through a wound-healing assay. Results Benzofuran (33.20%) emerged as the major compound of CAEO, followed by Germacrene B (19.12%) and Germacrone (13.60%). Two types of CAEO loaded nano-formulations, nanoemulsion (NE) and microemulsion (ME) were developed. The average droplet sizes of NE and ME were 13.8 ± 0.2 and 21.2 ± 0.2 nm, respectively. In a comparison with other essential oils from the fresh rhizomes of potential plants from the same family (Curcuma longa, Curcuma mangga and Zingiber officinale) on anticancer activity against K562 and MCF-7 cell lines, CAEO exhibited the highest cytotoxicity with IC50 of 13.43 ± 1.09 and 20.18 ± 1.20 µg/mL, respectively. Flow cytometry analysis revealed that CAEO significantly increased cell death, evidenced from the sub-G1 populations in the cell cycle assay and triggered apoptosis. Additionally, CAEO effectively inhibited cell migration in MCF-7 cells after incubation for 12 and 24 h. The developed NE and ME formulations significantly enhanced the cytotoxicity of CAEO against K562 cells with an IC50 of 45.30 ± 1.49 and 41.98 ± 0.96 µg/mL, respectively. Conclusion This study’s finding suggest that both nano-formulations, NE and ME, effectively facilitated the delivery of CAEO into cancer cells. |
| first_indexed | 2024-03-08T16:24:12Z |
| format | Article |
| id | doaj.art-4c1a8a3554564a02aae8bf3930a9cdac |
| institution | Directory Open Access Journal |
| issn | 2662-7671 |
| language | English |
| last_indexed | 2024-03-08T16:24:12Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Complementary Medicine and Therapies |
| spelling | doaj.art-4c1a8a3554564a02aae8bf3930a9cdac2024-01-07T12:10:53ZengBMCBMC Complementary Medicine and Therapies2662-76712024-01-0124111310.1186/s12906-023-04261-9Anticancer activity of Curcuma aeroginosa essential oil and its nano-formulations: cytotoxicity, apoptosis and cell migration effectsPawaret Panyajai0Natsima Viriyaadhammaa1Singkome Tima2Sawitree Chiampanichayakul3Pornngarm Dejkriengkraikul4Siriporn Okonogi5Songyot Anuchapreeda6Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai UniversityDepartment of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai UniversityDepartment of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai UniversityDepartment of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai UniversityDepartment of Biochemistry, Faculty of Medicine, Chiang Mai UniversityCenter of Excellence in Pharmaceutical Nanotechnology, Chiang Mai UniversityDepartment of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai UniversityAbstract Background and aims Curcuma aeruginosa, commonly known as “kha-min-dam” in Thai, holds significance in Asian traditional medicine due to its potential in treating various diseases, having properties such as anti-HIV, hepatoprotective, antimicrobial and anti-androgenic activities. This study explores the anticancer activity of C. aeruginosa essential oil (CAEO) and its nano-formulations. Methods CAEO obtained from hydrodistillation of C. aeruginosa fresh rhizomes was examined by gas chromatography mass spectroscopy. Cytotoxicity of CAEO was determined in leukaemic K562 and breast cancer MCF-7 cell lines using an MTT assay. Cell cycle analysis and cell apoptosis were determined by flow cytometry. Cell migration was studied through a wound-healing assay. Results Benzofuran (33.20%) emerged as the major compound of CAEO, followed by Germacrene B (19.12%) and Germacrone (13.60%). Two types of CAEO loaded nano-formulations, nanoemulsion (NE) and microemulsion (ME) were developed. The average droplet sizes of NE and ME were 13.8 ± 0.2 and 21.2 ± 0.2 nm, respectively. In a comparison with other essential oils from the fresh rhizomes of potential plants from the same family (Curcuma longa, Curcuma mangga and Zingiber officinale) on anticancer activity against K562 and MCF-7 cell lines, CAEO exhibited the highest cytotoxicity with IC50 of 13.43 ± 1.09 and 20.18 ± 1.20 µg/mL, respectively. Flow cytometry analysis revealed that CAEO significantly increased cell death, evidenced from the sub-G1 populations in the cell cycle assay and triggered apoptosis. Additionally, CAEO effectively inhibited cell migration in MCF-7 cells after incubation for 12 and 24 h. The developed NE and ME formulations significantly enhanced the cytotoxicity of CAEO against K562 cells with an IC50 of 45.30 ± 1.49 and 41.98 ± 0.96 µg/mL, respectively. Conclusion This study’s finding suggest that both nano-formulations, NE and ME, effectively facilitated the delivery of CAEO into cancer cells.https://doi.org/10.1186/s12906-023-04261-9Curcuma aeroginosaEssential oilNano-formulationsAnticancerCytotoxicityApoptosis |
| spellingShingle | Pawaret Panyajai Natsima Viriyaadhammaa Singkome Tima Sawitree Chiampanichayakul Pornngarm Dejkriengkraikul Siriporn Okonogi Songyot Anuchapreeda Anticancer activity of Curcuma aeroginosa essential oil and its nano-formulations: cytotoxicity, apoptosis and cell migration effects BMC Complementary Medicine and Therapies Curcuma aeroginosa Essential oil Nano-formulations Anticancer Cytotoxicity Apoptosis |
| title | Anticancer activity of Curcuma aeroginosa essential oil and its nano-formulations: cytotoxicity, apoptosis and cell migration effects |
| title_full | Anticancer activity of Curcuma aeroginosa essential oil and its nano-formulations: cytotoxicity, apoptosis and cell migration effects |
| title_fullStr | Anticancer activity of Curcuma aeroginosa essential oil and its nano-formulations: cytotoxicity, apoptosis and cell migration effects |
| title_full_unstemmed | Anticancer activity of Curcuma aeroginosa essential oil and its nano-formulations: cytotoxicity, apoptosis and cell migration effects |
| title_short | Anticancer activity of Curcuma aeroginosa essential oil and its nano-formulations: cytotoxicity, apoptosis and cell migration effects |
| title_sort | anticancer activity of curcuma aeroginosa essential oil and its nano formulations cytotoxicity apoptosis and cell migration effects |
| topic | Curcuma aeroginosa Essential oil Nano-formulations Anticancer Cytotoxicity Apoptosis |
| url | https://doi.org/10.1186/s12906-023-04261-9 |
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